MUC5AC expression in human middle ear epithelium of patients with otitis media

Archives of Otolaryngology - Head and Neck Surgery

Volumn 136, Issue 8, 2010, Pages 819-824

  Kerschner, Joseph E ^a   Tripathi, Shivani ^b   Khampang, Pawjai ^a   Papsin, Blake C ^c  

^a Children's Hospital of Wisconsin   (United States)

^b MEDICAL COLLEGE OF WISCONSIN   (United States)

^c HOSPITAL FOR SICK CHILDREN   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

MUCIN 5AC;

ARTICLE; BIOPSY; CHILD; CHRONIC SECRETORY OTITIS MEDIA; CLINICAL ARTICLE; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; HUMAN; IMPLANTATION; INFANT; MALE; MIDDLE EAR; OTITIS MEDIA; PRESCHOOL CHILD; PROTEIN EXPRESSION; REAL TIME POLYMERASE CHAIN REACTION; RECURRENT DISEASE; RECURRENT OTITIS MEDIA; RNA EXTRACTION; SCHOOL CHILD; TYMPANOSTOMY TUBE;

CHILD; CHILD, PRESCHOOL; CHRONIC DISEASE; COCHLEAR IMPLANTATION; EAR, MIDDLE; ENZYME-LINKED IMMUNOSORBENT ASSAY; EPITHELIUM; FEMALE; GENE EXPRESSION; HUMANS; IMMUNOENZYME TECHNIQUES; INFANT; MALE; MIDDLE EAR VENTILATION; MUCIN 5AC; OTITIS MEDIA; OTITIS MEDIA WITH EFFUSION; RECURRENCE; REFERENCE VALUES; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION;

EID: 77955720284     PISSN: 08864470     EISSN: 1538361X     Source Type: Journal    
DOI: 10.1001/archoto.2010.123     Document Type: Article

References (17)

1. Kerschner JE. Mucin gene expression in human middle ear epithelium: American Laryngological, Rhinological and Otological Society thesis. Laryngoscope. 2007;117(9):1666-1676.
2. Shen H, Yoshida H, Yan F, et al. Synergistic induction of MUC5AC mucin by non-typeable Haemophilus influenzae and Steptococcus pneumoniae. Biochem Biophys Res Commun. 2008;365(4):795-800.
3. Kerschner JE, Meyer TK, Burrows A. Chinchilla middle ear epithelial mucin gene expression in response to inflammatory cytokines. Arch Otolaryngol Head Neck Surg. 2004;130(10):1163-1167.
4. Ubell M, Kerschner JE, Wackym A, Burrows A.MUC2 expression in human middle ear epithelium of patients with otitis media. Arch Otolaryngol Head Neck Surg. 2008;134(1):39-44.
5. Livak KJ, Schmittgen TD. Analysis of relative gene expression data using realtime quantitative PCR and the 2(-Delta Delta C (T)) method. Methods. 2001; 25(4):402-408.
6. Kerschner JE, Meyer T, Yang C, Burrows A. Middle ear epithelial mucin production in response to interleukin-6 exposure in vitro. Cytokine. 2004;26(1):30-36. (Pubitemid 38317412)
7. Lin J, Tsuprun V, Kawano H, et al. Characterization of mucins in human middle ear and Eustachian tube. Am J Physiol Lung Cell Mol Physiol. 2001;280(6): L1157-L1167.
8. Chung MH, Choi JY, Lee WS, Kim HN, Yoon JH. Compositional difference in middle ear effusion: mucous versus serous. Laryngoscope. 2002;112(1):152-155.
9. Lin J, Tsuboi Y, Rimell F, et al. Expression of mucins in mucoid otitis media. J Assoc Res Otolaryngol. 2003;4(3):384-393.
10. Elsheikh MN, Mahfouz ME. Up-regulation of MUC5AC and MUC5B mucin genes in nasopharyngeal respiratory mucosa and selective up-regulation of MUC5B in middle ear in pediatric otitis media with effusion. Laryngoscope. 2006;116 (3):365-369.
11. Voynow JA, Gendler SJ, Rose MC. Regulation of mucin genes in chronic inflammatory airway diseases. Am J Respir Cell Mol Biol. 2006;34(6):661-665.
12. Willett DN, Rezaee RP, Billy JM, Tighe MB, DeMaria TF. Relationship of endotoxin to tumor necrosis factor-α and interleukin-1β in children with otitis media with effusion. Ann Otol Rhinol Laryngol. 1998;107(1):28-33.
13. Yellon RF, Doyle WJ, Whiteside TL, Diven WF, March AR, Fireman P. Cytokines, immunoglobulins, and bacterial pathogens in middle ear effusions. Arch Otolaryngol Head Neck Surg. 1995;121(8):865-869.
14. Maxwell KS, Fitzgerald JE, Burleson JA, Leonard G, Carpenter R, Kreutzer DL. Interleukin-8 expression in otitis media. Laryngoscope. 1994;104(8, pt 1):989-995.
15. Smirnova MG, Birchall JP, Pearson JP. TNF-alpha in the regulation of MUC5AC secretion: some aspects of cytokine-induced mucin hypersecretion on the in vitro model. Cytokine. 2000;12(11):1732-1736.
16. Smirnova MG, Kiselev SL, Birchall JP, Pearson JP. Up-regulation of mucin secretion in HT29-MTX cells by the pro-inflammatory cytokines tumor necrosis factoralpha and interleukin-6. Eur Cytokine Netw. 2001;12(1):119-125.
17. Komatsu K, Jono H, Lim JH, et al. Glucocorticoids inhibit nontypeable Haemophilus influenzae-induced MUC5AC mucin expression via MAPK phosphatase-1 dependent inhibition of p38 MAPK. Biochem Biophys Res Commun. 2008;377 (3):763-768.