In vitro phage display in a rat beta cell line: A simple approach for the generation of a single-chain antibody targeting a novel beta cell-specific epitope

Diabetologia

Volumn 53, Issue 7, 2010, Pages 1384-1394

  Ueberberg, S ^a   Ziegler, D ^a   Schechinger, W ^a   Dietrich, J W ^a   Akinturk, S ^b   Klein, H H ^a   Schneider, S ^a  

^a RUHR UNIVERSITY BOCHUM   (Germany)

^b RUHR UNIVERSITY BOCHUM   (Germany)

Author keywords

AR42J cells; Beta cell; Human islets; INS 1 cells; Phage display; Single chain antibody

Indexed keywords

AMYLIN; BIOLOGICAL MARKER; C PEPTIDE; EPITOPE; GLUTAMATE DECARBOXYLASE; INSULIN; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY;

ANIMAL CELL; ANIMAL TISSUE; ANTIBODY SPECIFICITY; ARTICLE; BINDING AFFINITY; BINDING COMPETITION; BINDING SITE; CELL SURFACE; CONTROLLED STUDY; DIABETES MELLITUS; EXOCRINE CELL; FEMALE; HUMAN; HUMAN TISSUE; MALE; NONHUMAN; PANCREAS ISLET BETA CELL; PHAGE DISPLAY; PREDICTION; PRIORITY JOURNAL; RAT;

ANIMALS; CELL LINE; EPITOPES; HUMANS; IMMUNOHISTOCHEMISTRY; INSULIN-SECRETING CELLS; MICROSCOPY, FLUORESCENCE; PEPTIDE LIBRARY; RATS; SINGLE-CHAIN ANTIBODIES;

EID: 77955662125     PISSN: 0012186X     EISSN: 14320428     Source Type: Journal    
DOI: 10.1007/s00125-010-1725-9     Document Type: Article

References (23)

1. Weir GC, Bonner-Weier S, Leahy JL (1990) Islet mass and function in diabetes and transplantation. Diabetes 39:401-405.
2. Butler AE, Janson J, Bonner-Weier S et al (2003) β-cell deficit and increased β-cell apoptosis in humans with type 2 diabetes. Diabetes 52:102-110.
3. Schneider S (2008) Efforts to develop methods for in vivo evaluation of the native β-cell mass. Diabetes Obes Metab 10 (Suppl 4):109-118.
4. Schneider S, Feilen PJ, Schreckenberger M et al (2005) In vitro and in vivo evaluation of novel glibenclamide derivatives as imaging agents for the non-invasive assessment of the pancreatic islet cell mass in animals and humans. Exp Clin Endocrinol Diabetes 113:388-395.
5. Schneider S, Ueberberg S, Korobeynikov A et al (2007) Synthesis and evaluation of a glibenclamide glucose-conjugate: a potential new lead compound for substituted glibenclamide derivatives as islet imaging agents. Regul Pept 139:122-127.
6. Wängler B, Schneider S, Thews O et al (2004) Synthesis and evaluation of [18F]repaglinide: a promising radioligand for quantification of pancreatic β-cell mass with positron emission tomography (PET). Nucl Med Biol 31:639-647.
7. Sweet IR, Cook DL, Lernmark A et al (2004) Systematic screening of potential beta cell imaging agents. Biochem Biophys Res Commun 314:976-983.
8. Hampe CS, Wallen AR, Schlosser M, Ziegler M, Sweet IR (2005) Quantitative evaluation of a monoclonal antibody and its fragment as potential markers for pancreatic β-cell mass. Exp Clin Endocrinol Diabetes 113:381-387.
9. Gotthardt M, Lalyko G, van Eerd-Vismale J et al (2004) A new technique for in vivo imaging of specific GLP-1 binding sites: first results in small rodents. Regul Pept 137:162-167.
10. Souza F, Simpson N, Raffo A et al (2006) Longitudinal noninvasive PET-based beta cell mass estimates in a spontaneous diabetes rat model. J Clin Invest 116:1506-1513.
11. Moore A, Bonner-Weir S, Weissleder R (2001) Noninvasive in vivo measurement of beta cell mass in mouse model of diabetes. Diabetes 50:2231-2236.
12. Malaisse WJ (2001) On the track to the β-cell. Diabetologia 44:393-406.
13. Souza F, Freeby M, Hultman K et al (2006) Current progress in non-invasive imaging of beta cell mass of the endocrine pancreas. Curr Med Chem 13:2761-2773.
14. Virostko J, Jansen ED, Powers AC (2006) Current status of imaging pancreatic islets. Curr Diab Rep 6:328-332.
15. Ueberberg S, Schneider S (2010) Phage library-screening: a powerful approach for generation of targeting-agents specific for normal pancreatic islet-cells and islet-cell carcinoma in vivo. Regul Pept 160:1-8.
16. Kolonin MG, Saha PK, Chan L, Pasqualini R, Arap W (2004) Reversal of obesity by targeted ablation of adipose tissue. Nat Med 10:625-632.
17. Arap W, Kolonin MG, Trepel M et al (2002) Steps toward mapping the human vasculature by phage display. Nat Med 8:121-127.
18. Ueberberg S, Meier JJ, Waengler C et al (2009) Generation of novel single-chain antibodies by phage-display technology to direct imaging agents highly selective to pancreatic beta- or alphacells in vivo. Diabetes 58:2324-34.
19. Pasqualini R, Arap W, Rajotte D, Ruoslathi E (2000) In vivo selection of phage display libraries. In: Barbas C, Burton D, Silverma G, Scott J (eds) Phage display: a laboratory manual. Cold Spring Harbor Laboratory Press, New York, pp 22.1-22.24.
20. Giordano RJ, Marina CV, Lahdenranta J, Pasqualini R, Arap W (2001) Biopanning and rapid analysis of selective interactive ligands. Nat Med 7:1249-1253.
21. Samli KN, McGuire MJ, Newgard CB, Johnston SA, Brown KC (2005) Peptide-mediated targeting of the islets of Langerhans. Diabetes 54:2103-2108.
22. Joyce JA, Laakkonen P, Bernasconi M, Bergers G, Ruoslahti E, Hanahan D (2003) Stage-specific vascular markers revealed by phage display in a mouse model of pancreatic islet tumorigenesis. Cancer Cell 4:393-403.
23. Yao VJ, Yao VJ, Ozawa MG, Trepel M, Arap W, McDonald DM, Pasqualini R (2005) Targeting pancreatic islets with phage display assisted by laser pressure catapult microdissection. Am J Pathol 166:625-636.