Detection window of Darbepoetin-α following one single subcutaneous injection

Clinica Chimica Acta

Volumn 379, Issue 1-2, 2007, Pages 145-149

  Lamon, Séverine ^a   Robinson, Neil ^a   Mangin, Patrice ^a   Saugy, Martial ^a  

^a UNIVERSITY CENTER OF LEGAL MEDICINE   (Switzerland)

Author keywords

Detection window; Doping; Erythropoietin; NESP; Urine

Indexed keywords

ERYTHROPOIETIN; NOVEL ERYTHROPOIESIS STIMULATING PROTEIN; RECOMBINANT ERYTHROPOIETIN;

ADULT; ALTITUDE; ARTICLE; EVALUATION; HUMAN; HYPOXIA; ISOELECTRIC POINT; NORMAL HUMAN; PRIORITY JOURNAL; TRAINING;

ADULT; DOPING IN SPORTS; ERYTHROPOIETIN; HEMATINICS; HUMANS; INJECTIONS, SUBCUTANEOUS; MALE; SUBSTANCE ABUSE DETECTION;

EID: 33847685805     PISSN: 00098981     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.cca.2007.01.014     Document Type: Article

References (21)

1. Fisher J.W. Erythropoietin: physiology and pharmacology update. Exp Biol Med (Maywood) 228 (2003) 1-14
2. Egrie J., Grant J., Gillies D., Aoki K., and Strickland T. The role of carbohydrate on the biological activity of erythropoietin. Glycoconj J 10 (1993) 263
3. Egrie J.C., and Browne J.K. Development and characterization of novel erythropoiesis stimulating protein (NESP). Br J Cancer 84 Suppl 1 (2001) 3-10
4. Egrie J.C., Dwyer E., Browne J.K., Hitz A., and Lykos M.A. Darbepoetin alfa has a longer circulating half-life and greater in vivo potency than recombinant human erythropoietin. Exp Hematol 31 (2003) 290-299
5. Lasne F., Martin L., Crepin N., and de Ceaurriz J. Detection of isoelectric profiles of erythropoietin in urine: differentiation of natural and administered recombinant hormones. Anal Biochem 311 (2002) 119-126
6. Lasne F. Double-blotting: a solution to the problem of nonspecific binding of secondary antibodies in immunoblotting procedures. J Immunol Methods 276 (2003) 223-226
7. Khan A., Grinyer J., Truong S.T., Breen E.J., and Packer N.H. New urinary EPO drug testing method using two-dimensional gel electrophoresis. Clin Chim Acta 358 (2005) 119-130
8. Rabin O.P., Lasne F., Pascual J.A., Saugy M., Delbeke F.J., and Van E.P. New urinary EPO drug testing method using two-dimensional gel electrophoresis. Clin Chim Acta 373 (2006) 186-187
9. Wide L., Wikstrom B., and Eriksson K. A new principle suggested for detection of darbepoetin-alpha (NESP) doping. Ups J Med Sci 108 (2003) 229-238
10. Breidbach A., Catlin D.H., Green G.A., Tregub I., Truong H., and Gorzek J. Detection of recombinant human erythropoietin in urine by isoelectric focusing. Clin Chem 49 (2003) 901-907
11. Catlin D.H., Breidbach A., Elliott S., and Glaspy J. Comparison of the isoelectric focusing patterns of darbepoetin alfa, recombinant human erythropoietin, and endogenous erythropoietin from human urine. Clin Chem 48 (2002) 2057-2059
12. Bajla I., Hollander I., Minichmayr M., Gmeiner G., and Reichel C. GASepo-a software solution for quantitative analysis of digital images in Epo doping control. Comput Methods Programs Biomed 80 (2005) 246-270
13. Lasne F., Popot M.A., Varlet-Marie E., et al. Detection of recombinant epoetin and darbepoetin alpha after subcutaneous administration in the horse. J Anal Toxicol 29 (2005) 835-837
14. Macdougall I.C. CERA (Continuous Erythropoietin Receptor Activator): a new erythropoiesis-stimulating agent for the treatment of anemia. Curr Hematol Rep 4 (2005) 436-440
15. Saugy M., Robinson N., Pfister Y., and Mangin P. One year's experiment with the blood screening test to fight against rhEPO doping. Proceedings of the Manfred Donike Workshop 20th Cologne Workshop on Dope Analysis vol. 10 (2002) 159-168
16. Jelkmann W. Erythropoietin: structure, control of production, and function. Physiol Rev 72 (1992) 449-489
17. Ashenden M., Varlet-Marie E., Lasne F., and Audran M. The effects of microdose recombinant human erythropoietin regimens in athletes. Haematologica 91 (2006) 1143-1144
18. Klausen T., Dela F., Hippe E., and Galbo H. Diurnal variations of serum erythropoietin in trained and untrained subjects. Eur J Appl Physiol Occup Physiol 67 (1993) 545-548
19. Robinson N. Blood manipulation in endurance sports. 2003. Ph.D. thesis, University of Lausanne.
20. Emslie K.R., Howe C., and Trout G. Measurement of urinary erythropoeitin levels in athletes. Proceedings of the Manfred Donike Workshop 17th Cologne Workshop on Dope Analysis vol. 7 (2002) 291-299
21. Beullens M., Delanghe J.R., and Bollen M. False-positive detection of recombinant human erythropoietin in urine following strenuous physical exercise. Blood 107 (2006) 4711-4713