High-level expression of functional recombinant human butyrylcholinesterase in silkworm larvae by Bac-to-Bac system

Chemico-Biological Interactions

Volumn 187, Issue 1-3, 2010, Pages 101-105

  Li, Shuo ^a   Ip, Denis Tsz Ming ^a   Lin, Huang Quan ^a   Liu, Jian Mei ^b   Miao, Yun Gen ^b   Ke, Li Jian ^a   Wan, David Chi Cheong ^a  

^a and Hong Kong Branch of CAS Center for Excellence in Animal Evolution and Genetics   (Hong Kong)

^b ZHEJIANG UNIVERSITY   (China)

Author keywords

Bac to Bac system; Butyrylcholinesterase; Purification; Silkworm (Bombyx mori L.)

Indexed keywords

CHOLINESTERASE; COMPLEMENTARY DNA; FLUORESCENT DYE; ORANGE FLUORESCENT PROTEIN; RECOMBINANT ENZYME; UNCLASSIFIED DRUG;

ARTICLE; BACULOVIRUS EXPRESSION SYSTEM; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME SYNTHESIS; FLUORESCENCE ANALYSIS; GENETIC TRANSFECTION; HEMOLYMPH; ILLUMINATION; LARVA; MOLECULAR CLONING; NONHUMAN; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROMOTER REGION; PROTEIN EXPRESSION; SILKWORM; WESTERN BLOTTING;

BOMBYX MORI; CAPRA HIRCUS;

EID: 77955513248     PISSN: 00092797     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.cbi.2010.03.055     Document Type: Article

References (15)

1. Mattes C.E., Lynch T.J., Singh A., Bradley R.M., Kellaris P.A., Brady R.O., Dretchen K.L. Therapeutic use of butyrylcholinesterase for cocaine intoxication. Toxicol. Appl. Pharmacol. 1997, 145(2):372-380.
2. Lockridge O. Genetic variants of human serum cholinesterase influence metabolism of the muscle relaxant succinylcholine. Pharmacol. Ther. 1990, 47(1):35-60.
3. Viby-Mogensen J. Succinylcholine neuromuscular blockade in subjects heterozygous for abnormal plasma cholinesterase. Anesthesiology 1981, 55(3):231-235.
4. Raveh L., Grunwald J., Marcus D., Papier Y., Cohen E., Ashani Y. Human butyrylcholinesterase as a general prophylactic antidote for nerve agent toxicity. In vitro and in vivo quantitative characterization. Biochem. Pharmacol. 1993, 45(12):2465-2474.
5. Ashani Y., Shapira S., Levy D., Wolfe A.D., Doctor B.P., Raveh L. Butyrylcholinesterase and acetylcholinesterase prophylaxis against soman poisoning in mice. Biochem. Pharmacol. 1991, 41(1):37-41.
6. Allon N., Raveh L., Gilat E., Cohen E., Grunwald J., Ashani Y. Prophylaxis against soman inhalation toxicity in guinea pigs by pretreatment alone with human serum butyrylcholinesterase. Toxicol. Sci. 1998, 43(2):121-128.
7. Lockridge O., Schopfer L.M., Winger G., Woods J.H. Large scale purification of butyrylcholinesterase from human plasma suitable for injection into monkeys; a potential new therapeutic for protection against cocaine and nerve agent toxicity. J. Med. Chem. Biol. Radiol. Def. 2005, 3:nihms5095.
8. Podoly E., Bruck T., Diamant S., Melamed-Book N., Weiss A., Huang Y., Livnah O., Langermann S., Wilgus H., Soreq H. Human recombinant butyrylcholinesterase purified from the milk of transgenic goats interacts with beta-amyloid fibrils and suppresses their formation in vitro. Neurodegener. Dis. 2008, 5(3/4):232-236.
9. Ngamelue M.N., Homma K., Lockridge O., Asojo O.A. Crystallization and X-ray structure of full-length recombinant human butyrylcholinesterase. Acta Crystallogr. F: Struct. Biol. Cryst. Commun. 2007, 63(Pt 9):723-727.
10. Wei W.L., Qin J.C., Sun M.J. High-level expression of human butyrylcholinesterase gene in Bombyx mori and biochemical-pharmacological characteristic study of its product. Biochem. Pharmacol. 2000, 60(1):121-126.
11. Miao Y., Zhang Y., Nakagaki K., Zhao T., Zhao A., Meng Y., Nakagaki M., Park E.Y., Maenaka K. Expression of spider flagelliform silk protein in Bombyx mori cell line by a novel Bac-to-Bac/BmNPV baculovirus expression system. Appl. Microbiol. Biotechnol. 2006, 71(2):192-199.
12. Ip D.T., Wong K.B., Wan D.C. Characterization of novel orange fluorescent protein cloned from cnidarian tube anemone Cerianthus sp. Mar. Biotechnol. (NY) 2007, 9(4):469-478.
13. Lin H.Q., Ho M.T., Lau L.S., Wong K.K., Shaw P.C., Wan D.C. Anti-acetylcholinesterase activities of traditional Chinese medicine for treating Alzheimer's disease. Chem. Biol. Interact. 2008, 175(1-3):352-354.
14. Grunwald J., Marcus D., Papier Y., Raveh L., Pittel Z., Ashani Y. Large-scale purification and long-term stability of human butyrylcholinesterase: a potential bioscavenger drug. J. Biochem. Biophys. Methods 1997, 34(2):123-135.
15. Radic Z., Pickering N.A., Vellom D.C., Camp S., Taylor P. Three distinct domains in the cholinesterase molecule confer selectivity for acetyl- and butyrylcholinesterase inhibitors. Biochemistry 1993, 32(45):12074-12084.