High-level expression of human butyrylcholinesterase gene in Bombyx mori and biochemical-pharmacological characteristic study of its product

Biochemical Pharmacology

Volumn 60, Issue 1, 2000, Pages 121-126

  Wei, Wan Li ^a   Qin, Jun Chuan ^b   Sun, Man Ji ^a  

^a BEIJING INSTITUTE OF MICROBIOLOGY AND EPIDEMIOLOGY   (China)

^b NANJING UNIVERSITY   (China)

Author keywords

Baculovirus; Bombyx mori; Butyrylcholinesterase; Gene expression; Sarin

Indexed keywords

CHOLINESTERASE; RECOMBINANT ENZYME; VIRUS VECTOR;

ANIMAL CELL; ARTICLE; CHO CELL; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; GENE TRANSFER; HEMOLYMPH; HUMAN; MOLECULAR WEIGHT; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; SILKWORM; WESTERN BLOTTING;

ANIMALS; ANTIBODIES; BACULOVIRIDAE; BOMBYX; BUTYRYLCHOLINESTERASE; CELLS, CULTURED; CHOLINESTERASE INHIBITORS; CHOLINESTERASE REACTIVATORS; CLONING, MOLECULAR; CRICETINAE; DRUG INTERACTIONS; GENE EXPRESSION; HUMANS; LARVA; PYRIDINIUM COMPOUNDS; RABBITS; SARIN;

EID: 0034009587     PISSN: 00062952     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-2952(00)00238-0     Document Type: Article

References (10)

1. Mattes C.E., Lynch T.J., Singh A., Bradley R.M., Kellaris P.A., Brady R.O., Dretchen K.L. Therapeutic use of butyrylcholinesterase for cocaine intoxication. Toxicol Appl Pharmacol. 145:1997;372-380.
2. Lockridge O. Genetic variants of human serum cholinesterase influence metabolism of the muscle relaxant succinylcholine. Pharmacol Ther. 47:1990;35-60.
3. Allon N., Raveh L., Gilat E., Cohen E., Grunwald J., Ashani Y. Prophylaxis against soman inhalation toxicity in guinea pigs by pretreatment alone with human serum butyrylcholinesterase. Toxicol Sci. 43:1998;121-128.
4. Raveh L., Grauer E., Grunwald J., Cohen E., Ashani Y. The stoichiometry of protection against soman and VX toxicity in monkeys pretreated with human butyrylcholinesterase. Toxicol Appl Pharmacol. 145:1997;43-53.
5. Masson P, Expression and refolding of functional human BChE from E. Coli. In: Multiple Approaches to Cholinesterase Functions (Eds. Shafferman A and Velan B), pp. 49-52. Plenum, New York, 1990.
6. Soreq H., Seidman S., Dreyfus P.A., Zevin-Sonkin D., Zakut H. Expression and tissue-specific assembly of human butyrylcholine esterase in microinjected Xenopus laevis oocytes. J Biol Chem. 264:1989;10608-10613.
7. Lockridge O, Human cholinesterase gene expression in mammalian cells. AD Report A 227610.MF, USA, 1990.
8. Wang L., Qin J., Shen B. Expression of human stem cell factor in the baculovirus expression system. Biochem Mol Biol Int. 37:1995;729-736.
9. Karnoviky M.J., Root L.A. A "direct-coloring" thiocholine method for cholinesterases. J Histochem Cytochem. 12:1964;219-221.
10. Maeda S, Kawai and Obinata T, Production of human α-interferon in silkworm using a baculovirus vector. Nature315: 592-594, 1985.