Identification of activity related amino acid mutations of a GH9 termite cellulase

Bioresource Technology

Volumn 101, Issue 16, 2010, Pages 6438-6443

  Ni, Jinfeng ^a   Takehara, Motomi ^b   Watanabe, Hirofumi ^b  

^a SHANDONG UNIVERSITY   (China)

^b NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCES   (Japan)

Author keywords

Activity; Cellulase; Site directed mutagenesis; Termite

Indexed keywords

AMINO ACIDS; ENZYMES; MUTAGENESIS; THERMODYNAMIC PROPERTIES;

AMINO ACID RESIDUES; CARBOXY-METHYL CELLULOSE; CELLULASE; ENZYMATIC ACTIVITIES; ENZYMATIC CATALYSIS; RETICULITERMES SPERATUS; SITE DIRECTED MUTAGENESIS; TERMITE;

ENZYME ACTIVITY;

AMINO ACID; CELLULASE;

AMINO ACID; CATALYSIS; CELLULOSE; ENZYME ACTIVITY; GENE EXPRESSION; IDENTIFICATION METHOD; MUTATION; TERMITE;

ARTICLE; ENZYME ACTIVITY; ESCHERICHIA COLI; ISOPTERA; NONHUMAN; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; AMINO ACID SEQUENCE; ANIMAL; BIOCATALYSIS; CHEMISTRY; ENZYME ACTIVE SITE; GENETICS; METABOLISM; MOLECULAR GENETICS; SEQUENCE HOMOLOGY;

ISOPTERA; RETICULITERMES SPERATUS;

AMINO ACID SEQUENCE; AMINO ACIDS; ANIMALS; BIOCATALYSIS; CATALYTIC DOMAIN; CELLULASE; ESCHERICHIA COLI; ISOPTERA; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; SEQUENCE HOMOLOGY, AMINO ACID;

EID: 77955503215     PISSN: 09608524     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.biortech.2010.03.045     Document Type: Article

References (35)

1. Aharoni, A., Gaidukov, L., Yagur, S., Toker, L., Silman, I., Tawfik, D.S., 2004. Directed evolution of mammalian paraoxonases PON1 and PON3 for bacterial expression and catalytic specialization. Proc. Natl. Acad. Sci. USA 101, 482-487.
2. Boer, H., Simolin, H., Cottaz, S., Soderlund, H., Koivula, A., 2007. Heterologous expression and site-directed mutagenesis studies of two Trichoderma harzianum chitinases, Chit33 and Chit42, in Escherichia coli. Protein Expr. Purif. 51, 216-226.
3. Brune, A., 1998. Termite guts: the world's smallest bioreactors. Trends Biotechnol. 16, 16-21.
4. Chauvaux, S., Beguin, P., Aubert, J.P., 1992. Site-directed mutagenesis of essential carboxylic residues in Clostridium thermocellum endoglucanase CelD. J. Biol. Chem. 267, 4472-4478.
5. Crawford, A.C., Kricker, J.A., Anderson, A.J., Richardson, N.R., Mather, P.B., 2005. Structure and function of a cellulase gene in redclaw crayfish, Cherax quadricarinatus. Gene 340, 267-274.
6. Dale, G.E., Broger, C., Langen, H., D'Arcy, A., Stuber, D., 1994. Improving protein solubility through rationally designed amino acid replacements: solubilization of the trimethoprim-resistant type S1 dihydrofolate reductase. Protein Eng. 7, 933-939.
7. Droge, S., Frohlich, J., Radek, R., Konig, H., 2006. Spirochaeta coccoides sp. nov., a novel coccoid spirochete from the hindgut of the termite Neotermes castaneus. Appl. Environ. Microbiol. 72, 392-397.
8. Giorda, R., Ohmachi, T., Shaw, D.R., Ennis, H.L., 1990. A shared internal threonine-glutamic acid-threonine-proline repeat defines a family of Dictyostelium discoideum spore germination specific proteins. Biochemistry 29, 7264-7269.
9. Harel, M., Aharoni, A., Gaidukov, L., Brumshtein, B., Khersonsky, O., Meged, R., Dvir, H., Ravelli, R.B., McCarthy, A., Toker, L., Silman, I., Sussman, J.L., Tawfik, D.S., 2004. Structure and evolution of the serum paraoxonase family of detoxifying and anti-atherosclerotic enzymes. Nat. Struct. Mol. Biol. 11, 412-419.
10. Jue, C.K., Lipke, P.N., 1985. Determination of reducing sugars in the nanomole range with tetrazolium blue. J. Biochem. Biophys. Methods 11, 109-115.
11. Khademi, S., Guarino, L.A., Watanabe, H., Tokuda, G., Meyer, E.F., 2002. Structure of an endoglucanase from termite, Nasutitermes takasagoensis. Acta Crystallogr. D Biol. Crystallogr. 58, 653-659.
12. Malburg Jr., L.M., Iyo, A.H., Forsberg, C.W., 1996. A novel family 9 endoglucanase gene (celD), whose product cleaves substrates mainly to glucose, and its adjacent upstream homolog (celE) from Fibrobacter succinogenes S85. Appl. Environ. Microbiol. 62, 898-906.
13. McIlvaine, T.C., 1921. A buffer solution for colorimetric comparison. J. Biol. Chem. 49, 183-186.
14. Nakashima, K., Watanabe, H., Saitoh, H., Tokuda, G., Azuma, J.I., 2002a. Dual cellulose-digesting system of the wood-feeding termite, Coptotermes formosanus Shiraki. Insect Biochem. Mol. Biol. 32, 777-784.
15. Nakashima, K.I., Watanabe, H., Azuma, J.I., 2002b. Cellulase genes from the parabasalian symbiont Pseudotrichonympha grassii in the hindgut of the wood-feeding termite Coptotermes formosanus. Cell Mol. Life Sci. 59, 1554-1560.
16. Ni, J., Takehara, M., Miyazawa, M., Watanabe, H., 2007. Random exchanges of non-conserved amino acid residues among four parental termite cellulases by family shuffling improved thermostability. Protein Eng. Des. Sel. 20, 535-542.
17. Ni, J., Takehara, M., Watanabe, H., 2005. Heterologous overexpression of a mutant termite cellulase gene in Escherichia coli by DNA shuffling of four orthologous parental cDNAs. Biosci. Biotechnol. Biochem. 69, 1711-1720.
18. Ohkuma, M., 2003. Termite symbiotic systems: efficient bio-recycling of lignocellulose. Appl. Microbiol. Biotechnol. 61, 1-9.
19. Ohtoko, K., Ohkuma, M., Moriya, S., Inoue, T., Usami, R., Kudo, T., 2000. Diverse genes of cellulase homologues of glycosyl hydrolase family 45 from the symbiotic protists in the hindgut of the termite Reticulitermes speratus. Extremophiles 4, 343-349.
20. Ragauskas, A.J., Williams, C.K., Davison, B.H., Britovsek, G., Cairney, J., Eckert, C.A., Frederick Jr., W.J., Hallett, J.P., Leak, D.J., Liotta, C.L., Mielenz, J.R., Murphy, R., Templer, R., Tschaplinski, T., 2006. The path forward for biofuels and biomaterials. Science 311, 484-489.
21. Roodveldt, C., Aharoni, A., Tawfik, D.S., 2005. Directed evolution of proteins for heterologous expression and stability. Curr. Opin. Struct. Biol. 15, 50-56.
22. Sarkar, C.A., Dodevski, I., Kenig, M., Dudli, S., Mohr, A., Hermans, E., Pluckthun, A., 2008. Directed evolution of a G protein-coupled receptor for expression, stability, and binding selectivity. Proc. Natl. Acad. Sci. USA 105, 14808-14813.
23. Smialowski, P., Martin-Galiano, A.J., Mikolajka, A., Girschick, T., Holak, T.A., Frishman, D., 2007. Protein solubility: sequence based prediction and experimental verification. Bioinformatics 23, 2536-2542.
24. Suzuki, K.-I., Ojima, T., Nishita, K., 2003. Purification and cDNA cloning of a cellulase from abalone Haliotis discus hannai. Eur. J. Biochem. 270, 771-778.
25. Teather, R.M., Wood, P.J., 1982. Use of Congo red-polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen. Appl. Environ. Microbiol. 43, 777-780.
26. Todaka, N., Moriya, S., Saita, K., Hondo, T., Kiuchi, I., Takasu, H., Ohkuma, M., Piero, C., Hayashizaki, Y., Kudo, T., 2007. Environmental cDNA analysis of the genes involved in lignocellulose digestion in the symbiotic protist community of Reticulitermes speratus. FEMS Microbiol. Ecol. 59, 592-599.
27. Tokuda, G., Saito, H., Watanabe, H., 2002. A digestive beta-glucosidase from the salivary glands of the termite, Neotermes koshunensis (Shiraki): distribution, characterization and isolation of its precursor cDNA by 5′- and 3′-RACE amplifications with degenerate primers. Insect Biochem. Mol. Biol. 32, 1681-1689.
28. Tokuda, G., Watanabe, H., Matsumoto, T., Noda, H., 1997. Cellulose digestion in the wood-eating higher termite, Nasutitermes takasagoensis (Shiraki): distribution of cellulases and properties of endo-beta-1,4-glucanase. Zoolog. Sci. 14, 83-93.
29. Trainotti, L., Spolaore, S., Ferrarese, L., Casadoro, G., 1997. Characterization of ppEG1, a member of a multigene family which encodes endo-beta-1,4-glucanase in peach. Plant Mol. Biol. 34, 791-802.
30. Warnecke, F., Luginbuhl, P., Ivanova, N., Ghassemian, M., Richardson, T.H., Stege, J.T., Cayouette, M., McHardy, A.C., Djordjevic, G., Aboushadi, N., Sorek, R., Tringe, S.G., Podar, M., Martin, H.G., Kunin, V., Dalevi, D., Madejska, J., Kirton, E., Platt, D., Szeto, E., Salamov, A., Barry, K., Mikhailova, N., Kyrpides, N.C., Matson, E.G., Ottesen, E.A., Zhang, X., Hernandez, M., Murillo, C., Acosta, L.G., Rigoutsos, I., Tamayo, G., Green, B.D., Chang, C., Rubin, E.M., Mathur, E.J., Robertson, D.E., Hugenholtz, P., Leadbetter, J.R., 2007. Metagenomic and functional analysis of hindgut microbiota of a wood-feeding higher termite. Nature 450, 560-565.
31. Watanabe, H., Nakamura, M., Tokuda, G., Yamaoka, I., Scrivener, A.M., Noda, H., 1997. Site of secretion and properties of endogenous endo-beta-1,4-glucanase components from Reticulitermes speratus (Kolbe), a Japanese subterranean termite. Insect Biochem. Mol. Biol. 27, 305-313.
32. Watanabe, H., Noda, H., Tokuda, G., Lo, N., 1998. A cellulase gene of termite origin. Nature 394, 330-331.
33. Wenzel, M., Schonig, I., Berchtold, M., Kampfer, P., Konig, H., 2002. Aerobic and facultatively anaerobic cellulolytic bacteria from the gut of the termite Zootermopsis angusticollis. J. Appl. Microbiol. 92, 32-40.
34. Yamada, A., Inoue, T., Wiwatwitaya, D., Ohkuma, M., Kudo, T., Abe, T., Sugimoto, A., 2005. Carbon mineralization by termites in tropical forests, with emphasis on fungus combs. Ecol. Res. 20, 453-460.
35. Zhou, W., Irwin, D.C., Escovar-Kousen, J., Wilson, D.B., 2004. Kinetic studies of Thermobifida fusca Cel9A active site mutant enzymes. Biochemistry 43, 9655-9663.