Cloud-point extraction and delipidation of porcine brain proteins in combination with bottom-up mass spectrometry approaches for proteome analysis

Journal of Proteome Research

Volumn 9, Issue 8, 2010, Pages 3903-3911

  Shevchenko, Ganna ^a   Sjödin, Marcus O D ^a   Malmström, David ^a   Wetterhall, Magnus ^a   Bergquist, Jonas ^a  

^a UPPSALA UNIVERSITY   (Sweden)

Author keywords

bottomup proteomics; brain; central nervous system (CNS); cloud point extraction (CPE); delipidation; mass spectrometry (MS); membrane proteins (MPs)

Indexed keywords

BRAIN PROTEIN; DETERGENT; HYDROPHILIC PROTEIN; HYDROPHOBIC PROTEIN; LIPID; MEMBRANE PROTEIN; PEPTIDE; PROTEIN; PROTEOME; TRITON X 114; UNCLASSIFIED DRUG;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BRAIN TISSUE; CENTRAL NERVOUS SYSTEM; CLOUD POINT EXTRACTION; CONTROLLED STUDY; ELECTROSPRAY MASS SPECTROMETRY; ENZYME DEGRADATION; FIELD FLOW FRACTIONATION; GEL ELECTROPHORESIS; GENE; HYDROPHILICITY; HYDROPHOBICITY; IMMUNOPRECIPITATION; ISOELECTRIC FOCUSING; LIPID EXTRACTION; MASS SPECTROMETRY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NONHUMAN; ONE DIMENSIONAL GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN FUNCTION; PROTEOMICS; REVERSED PHASE LIQUID CHROMATOGRAPHY; SWINE; TEMPERATURE; TIME OF FLIGHT MASS SPECTROMETRY;

ANIMALS; BRAIN; CHEMICAL FRACTIONATION; CHROMATOGRAPHY, LIQUID; LIPIDS; NERVE TISSUE PROTEINS; PROTEOMICS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; SUS SCROFA; TANDEM MASS SPECTROMETRY; TEMPERATURE;

ANIMALIA; SUS;

EID: 77955441179     PISSN: 15353893     EISSN: 15353907     Source Type: Journal    
DOI: 10.1021/pr100116k     Document Type: Article

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