Multiple-yapsin-deficient mutant strains for high-level production of intact recombinant proteins in Saccharomyces cerevisiae

Journal of Biotechnology

Volumn 149, Issue 1-2, 2010, Pages 1-7

  Cho, Eun Young ^a,b   Cheon, Seon Ah ^c   Kim, Hyunah ^c   Choo, Jinho ^c   Lee, Dong Jik ^c   Ryu, Ho Myung ^d   Rhee, Sang Ki ^a   Chung, Bong Hyun ^a   Kim, Jeong Yoon ^b   Kang, Hyun Ah ^c  

^a Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

^b Chungnam National University   (South Korea)

^c Chung Ang University   (South Korea)

^d Dong Kook Pharmaceutical Co Ltd   (South Korea)

Author keywords

Proteolysis; Recombinant proteins; Secretory production; Yapsins

Indexed keywords

ASPARTIC PROTEASE; CELL SURFACES; CLEAVAGE SITES; DELETION MUTANTS; FED-BATCH CULTIVATION; GENES CODING; GROWTH CONDITIONS; GROWTH DEFECTS; HIGH-DENSITY; HIGH-LEVEL PRODUCTION; HYGROMYCIN; LATE STAGE; MUTANT STRAIN; NORMAL GROWTH; PARATHYROID HORMONES; PROTEOLYTIC CLEAVAGE; PROTEOLYTIC DEGRADATION; RECOMBINANT PROTEIN; RECOMBINANT PROTEINS; S.CEREVISIAE; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE MUTANT; SECRETORY PRODUCTION;

ALKALINITY; CELL MEMBRANES; YEAST;

PROTEINS;

FUNGAL ENZYME; FUNGAL PROTEIN; HYGROMYCIN B; RECOMBINANT PARATHYROID HORMONE; UNCLASSIFIED DRUG; YAPSIN 1; YAPSIN 2; YAPSIN 3; YAPSIN 6; YAPSIN 7;

ARTICLE; CELL SURFACE; CONTROLLED STUDY; DELETION MUTANT; FUNGAL CELL; FUNGAL STRAIN; FUNGUS GROWTH; GENE DISRUPTION; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN DEGRADATION; SACCHAROMYCES CEREVISIAE;

ASPARTIC ACID ENDOPEPTIDASES; HUMANS; PARATHYROID HORMONE; RECOMBINANT PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

SACCHAROMYCES CEREVISIAE;

EID: 77955419217     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2010.06.014     Document Type: Article

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