Enhanced expression of recombinant proteins utilizing a modified baculovirus expression vector

Molecular Biotechnology

Volumn 46, Issue 1, 2010, Pages 80-89

  Tiwari, Prabhakar ^a   Saini, Shalini ^a   Upmanyu, Sanatan ^a   Benjamin, Biju ^a   Tandon, Ruchi ^a   Saini, Kulvinder Singh ^a   Sahdev, Sudhir ^a  

^a RANBAXY RESEARCH LABORATORIES   (India)

Author keywords

AcMNPV; Bacterial artificial chromosome; Enhancer; Homologous region 1; Insect cells; Transposition

Indexed keywords

BACTERIAL ARTIFICIAL CHROMOSOMES; BACTERIAL EXPRESSION SYSTEMS; BACULOVIRUS; BACULOVIRUS EXPRESSION VECTOR SYSTEMS; COST OF PRODUCTIONS; EUKARYOTIC PROTEINS; EXPRESSION CONSTRUCTS; EXPRESSION VECTORS; HUMAN PROTEINS; INSECT CELL LINES; INSECT CELLS; LUCIFERASE GENES; MAMMALIAN CELLS; MULTIPLICITY OF INFECTIONS; N-TERMINALS; POST-TRANSLATIONAL MODIFICATIONS; PROTEIN CONCENTRATIONS; PROTEIN LEVEL; PROTEIN YIELD; RECOMBINANT PROTEIN; RECOMBINANT PROTEIN EXPRESSION; SPODOPTERA FRUGIPERDA; THERAPEUTIC APPLICATION; TIME INTERVAL; TRANSPORTATION PROPERTIES; TRICHOPLUSIA NI;

BACTERIOLOGY; CELL CULTURE; GENES; MAMMALS;

PROTEINS;

BACULOVIRUS VECTOR; CYTOCHROME P450 1A2; LUCIFERASE; PROTEIN KINASE B; RECOMBINANT PROTEIN;

ARTICLE; BACULOVIRUS; BACULOVIRUS EXPRESSION SYSTEM; CONTROLLED STUDY; DNA TRANSPOSITION; ENZYME ACTIVITY; EXPRESSION VECTOR; GENE EXPRESSION; GENE INSERTION; GENE TARGETING; HOMOLOGOUS RECOMBINATION; HUMAN; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN PROCESSING; PROTEIN TRANSPORT; REPORTER GENE;

BACULOVIRIDAE; CYTOCHROME P-450 CYP1A2; ENHANCER ELEMENTS, GENETIC; GENE EXPRESSION; GENES, REPORTER; GENETIC VECTORS; HUMANS; LUCIFERASES; MOLECULAR BIOLOGY; PROTO-ONCOGENE PROTEINS C-AKT; RECOMBINANT PROTEINS; RECOMBINATION, GENETIC;

AUTOGRAPHA CALIFORNICA NUCLEOPOLYHEDROVIRUS; BACTERIA (MICROORGANISMS); EUKARYOTA; HEXAPODA; MAMMALIA; SPODOPTERA FRUGIPERDA; TRICHOPLUSIA NI;

EID: 77955419022     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12033-010-9284-3     Document Type: Article

References (25)

1. BJ Kelly LA King RD Possee 2007 Introduction to baculovirus molecular biology Methods in Molecular Biology 388 25 54 10.1007/978-1-59745-457-5-2 1:CAS:528:DC%2BD2sXovFWltLo%3D
2. S Harwood 2007 Small-scale protein production with the baculovirus expression vector system Methods in Molecular Biology 388 211 224 10.1007/978-1-59745-457-5-10 1:CAS:528:DC%2BD2sXovFWltb4%3D
3. B Philipps D Rotmann M Wicki LM Mayr M Forstner 2005 Time reduction and process optimization of the baculovirus expression system for more efficient recombinant protein production in insect cells Protein Expression and Purification 42 211 218 10.1016/j.pep.2005.03.020 1:CAS:528:DC%2BD2MXltFeju7s%3D (Pubitemid 40793413)
4. KJ Airenne E Peltomaa VP Hytönen OH Laitinen S Ylä-Herttuala 2003 Improved generation of recombinant baculovirus genomes in Escherichia coli Nucleic Acids Research S31 e101 10.1093/nar/gng102
5. GP Pijlman J de Vrij FJ van den End JM Vlak DE Martens 2004 Evaluation of baculovirus expression vectors with enhanced stability in continuous cascaded insect-cell bioreactors Biotechnology and Bioengineering 87 743 753 10.1002/bit.20178 1:CAS:528:DC%2BD2cXnsVejt78%3D (Pubitemid 39264643)
6. GP Pijlman EC Roode X Fan LO Roberts GJ Belsham JM Vlak MM van Oers 2006 Stabilized baculovirus vector expressing a heterologous gene and GP64 from a single bicistronic transcript Journal of Biotechnology 123 13 21 10.1016/j.jbiotec.2005.10.022 1:CAS:528:DC%2BD28XjvVWlsLc%3D
7. MD Ayres SC Howard J Kuzio M Lopez-Ferber RD Possee 1994 The complete DNA sequence of Autographa californica nuclear polyhedrosis virus Virology 202 586 605 10.1006/viro.1994.1380 1:CAS:528:DyaK2cXmt1egtrw%3D (Pubitemid 24254872)
8. M Kool CH Ahrens JM Vlak GF Rohrmann 1995 Replication of baculovirus DNA Journal of General Virology 76 2103 2118 10.1099/0022-1317-76-9-2103 1:CAS:528:DyaK2MXnvVyqurs%3D
9. Ahrens, C. H., Leisy, D. J., & Rohrmann, G. F. (1996). DNA replication in eukaryotic cells (pp. 855-872). NY: Cold Spring Harbor Laboratory Press.
10. M Pearson R Bjornson G Pearsona G Rohrmann 1992 The Autographa californica baculovirus genome: evidence for multiple replication origins Science 257 1382 1384 10.1126/science.1529337 1:CAS:528:DyaK3sXkt1Wkurs%3D
11. M Kool JT Voeten RW Goldbach J Tramper JM Vlak 1993 Identification of seven putative origins of Autographa californica multiple nucleocapsid nuclear polyhedrosis virus DNA replication Journal of General Virology 74 2661 2668 10.1099/0022-1317-74-12-2661 1:CAS:528:DyaK2cXntVOltA%3D%3D (Pubitemid 24020659)
12. LA Guarino MD Summers 1986 Interspersed homologous DNA of Autographa californica nuclear polyhedrosis virus enhances delayed-early gene expression Journal of Virology 60 215 223 1:CAS:528:DyaL28XmtVeqt7o%3D
13. DD Carson MD Summers LA Guarino 1991 Transient expression of the Autographa californica nuclear polyhedrosis virus immediate-early gene, IE-N, is regulated by three viral elements Journal of Virology 65 945 951 1:CAS:528:DyaK3MXpsF2msA%3D%3D
14. S Habib S Pandey U Chatterji S Burma R Ahmad A Jain SE Hasnain 1996 Bifunctionality of the AcMNPV homologous region sequence (hr1): Enhancer and ori functions have different sequence requirements DNA and Cell Biology 15 737 747 10.1089/dna.1996.15.737 1:CAS:528:DyaK28XmtVygsbg%3D
15. S Habib SE Hasnain 1996 A 38-kDa host factor interacts with functionally important motifs within the Autographa californica multinucleocapsid nuclear polyhedrosis virus homologous region (hr1) DNA sequence The Journal of Biological Chemistry 271 28250 28258 10.1074/jbc.271.45.28250 1:CAS:528:DyaK28XmvVWkt7o%3D (Pubitemid 26374637)
16. S Sahdev S Saini P Tiwari S Saxena KS Saini 2007 Amplification of GC-rich genes by following a combination strategy of primer design, enhancers and modified PCR cycle conditions Molecular and Cellular Probes 4 303 307 10.1016/j.mcp.2007.03.004 (Pubitemid 46710344)
17. CW Fisher DL Caudle C Martin-Wixtrom LC Quattrochi RH Tukey MR Waterman RW Estabrook 1992 High-level expression of functional human cytochrome P450 1A2 in Escherichia coli FASEB Journal 6 759 764 1:CAS:528:DyaK38XhtVCnsb8%3D
18. L Ikonomou YJ Schneider SN Agathos 2003 Insect cell culture for industrial production of recombinant proteins Applied Microbiology and Biotechnology 62 1 20 10.1007/s00253-003-1223-9 1:CAS:528:DC%2BD3sXltVWhu7k%3D (Pubitemid 36836534)
19. S Naggie WE Bentley 1998 Appearance of protease activities coincides with p10 and polyhedrin-driven protein production in the baculovirus expression system: Effects on yield Biotechnology Progress 14 227 232 10.1021/bp980002b 1:CAS:528:DyaK1cXhs1Grs78%3D (Pubitemid 28202606)
20. CC Lin JT Hsu KL Huang HK Tang CW Shu YK Lai 2007 Sf-Caspase1-repressed stable cells: Resistance to apoptosis and augmentation of recombinant protein production Biotechnology and Applied Biochemistry 48 11 19 10.1042/BA20070044 1:CAS:528:DC%2BD2sXpsFOku7c%3D (Pubitemid 47425170)
21. A Fath-Goodin J Kroemer S Martin K Reeves BA Webb 2006 Polydnavirus genes that enhance the baculovirus expression vector system Advances in Virus Research 68 75 90 10.1016/S0065-3527(06)68002-0 1:CAS:528:DC%2BD1cXhs12lu7k%3D (Pubitemid 44429910)
22. T Kato T Murata T Usui EY Park 2003 Improvement of GFPuv-beta 3GnT2 fusion protein production by suppressing protease in baculovirus expression system Bioscience, Biotechnology, and Biochemistry 67 2388 2395 10.1271/bbb.67.2388 1:CAS:528:DC%2BD3sXps1emu7s%3D (Pubitemid 39251641)
23. LA Guarino W Dong 1994 Functional dissection of the Autographa californica nuclear polyhedrosis virus enhancer element hr5 Virology 200 328 335 10.1006/viro.1994.1197 1:CAS:528:DyaK2cXktVahtb8%3D (Pubitemid 24146510)
24. Y Chen B Yao Z Zhu Y Yi X Lin Z Zhang G Shen 2004 A constitutive super-enhancer: Homologous region 3 of Bombyx mori nucleopolyhedrovirus Biochemical and Biophysical Research Communications 318 1039 1044 10.1016/j.bbrc.2004.04.136 1:CAS:528:DC%2BD2cXktV2mu70%3D (Pubitemid 38625880)
25. LA Guarino MA Gonzalez MD Summers 1986 Complete sequence and enhancer function of the homologous DNA regions of Autographa californica nuclear polyhedrosis virus Journal of Virology 60 224 229 1:CAS:528:DyaL28XmtVeqt7s%3D