Time reduction and process optimization of the baculovirus expression system for more efficient recombinant protein production in insect cells

Protein Expression and Purification

Volumn 42, Issue 1, 2005, Pages 211-218

  Philipps, Björn ^a   Rotmann, Daniel ^a   Wicki, Micha ^a   Mayr, Lorenz M ^a   Forstner, Michael ^a  

^a NOVARTIS PHARMA AG   (Switzerland)

Author keywords

Baculovirus; BEVS; Centrifugation; Expression conditions; GFP; Insect cells; Protein expression

Indexed keywords

BETA GALACTOSIDASE; ENHANCED GREEN FLUORESCENT PROTEIN; GREEN FLUORESCENT PROTEIN; INTERLEUKIN 1 RECEPTOR ASSOCIATED KINASE; PHOSPHOTRANSFERASE; RECOMBINANT PROTEIN; SPHINGOSINE KINASE;

ANIMAL; ARMYWORM; ARTICLE; BACULOVIRUS; BIOSYNTHESIS; CELL LINE; CULTURE TECHNIQUE; ESCHERICHIA COLI; GENE EXPRESSION; GENE VECTOR; GENETIC TRANSFECTION; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METHODOLOGY; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PROMOTER REGION;

ANIMALS; BACULOVIRIDAE; BASE SEQUENCE; BETA-GALACTOSIDASE; CELL CULTURE TECHNIQUES; CELL LINE; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; HUMANS; INTERLEUKIN-1 RECEPTOR-ASSOCIATED KINASES; MOLECULAR SEQUENCE DATA; PHOSPHOTRANSFERASES (ALCOHOL GROUP ACCEPTOR); PROMOTER REGIONS (GENETICS); RECOMBINANT PROTEINS; SPODOPTERA; TRANSFECTION;

ESCHERICHIA COLI; INSECTA; UNIDENTIFIED BACULOVIRUS;

EID: 20444373770     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2005.03.020     Document Type: Article

References (19)

1. D.R. O'Reilly, L.K. Miller, and V.A. Luckow Baculovirus expression vectors 1994 Oxford University Press New York
2. B. Philipps, M. Forstner, and L.M. Mayr Baculovirus expression system for magnetic sorting of infected cells and enhanced titer determination Biotechniques 36 2004 80 83
3. H.J. Cha, T. Gotoh, and W.E. Bentley Simplification of titer determination for recombinant baculovirus by green fluorescent protein marker Biotechniques 23 1997 782 784 786
4. P. van Roessel, and A.H. Brand Imaging into the future: visualizing gene expression and protein interactions with fluorescent proteins Nat. Cell Biol. 4 2002 E15 E20
5. J. Lippincott-Schwartz, E. Snapp, and A. Kenworthy Studying protein dynamics in living cells Nat. Rev. Mol. Cell Biol. 2 2001 444 456
6. Y. Katagiri, and K.C. Ingham Enhanced production of green fluorescent fusion proteins in a baculovirus expression system by addition of secretion signal Biotechniques 33 2002 24 26
7. N. Kioukia, A.W. Nienow, A.N. Emery, and M. al-Rubeai Physiological and environmental factors affecting the growth of insect cells and infection with baculovirus J. Biotechnol. 38 1995 243 251
8. A. Giessauf, M. Flaim, M.P. Dierich, and R. Wurzner A technique for isolation of rubella virus-like particles by sucrose gradient ultracentrifugation using Coomassie brilliant blue G crystals Anal. Biochem. 308 2002 232 238
9. T. Kohno, S. Mohan, T. Goto, C. Morita, T. Nakano, W. Hong, J.C. Sangco, S. Morimatsu, and K. Sano A new improved method for the concentration of HIV-1 infective particles J. Virol. Methods 106 2002 167 173
10. L.A. King, and R.D. Possee The baculovirus expression system. A laboratory guide 1992 Chapman & Hall London
11. J.P. Condreay, S.M. Witherspoon, W.C. Clay, and T.A. Kost Transient and stable gene expression in mammalian cells transduced with a recombinant baculovirus vector Proc. Natl. Acad. Sci. USA 96 1999 127 132
12. C. Hofmann, V. Sandig, G. Jennings, M. Rudolph, P. Schlag, and M. Strauss Efficient gene transfer into human hepatocytes by baculovirus vectors Proc. Natl. Acad. Sci. USA 92 1995 10099 10103
13. T.P. Loisel, H. Ansanay, S. St-Onge, B. Gay, P. Boulanger, A.D. Strosberg, S. Marullo, and M. Bouvier Recovery of homogeneous and functional beta 2-adrenergic receptors from extracellular baculovirus particles Nat. Biotechnol. 15 1997 1300 1304
14. G. Zeng Sticky-end PCR: new method for subcloning Biotechniques 25 1998 206 208
15. L.J. Reed, and H. Muench A simple method of estimating fifty per cent endpoints Am. J. Hyg. 27 1938 493 497
16. P.R. Hughes, and H.A. Wood In vivo and in vitro bioassay methods for baculoviruses R.R. Granados B.A. Federici The biology of baculoviruses 1986 CRC Press Boca Raton, FL 1 30
17. R.M. Dougherty Animal virus titration techniques R. Harris Techniques in experimental virology 1964 Academic Press New York 169 223
18. T. Saito, T. Dojima, M. Toriyama, and E.Y. Park The effect of cell cycle on GFPuv gene expression in the baculovirus expression system J. Biotechnol. 93 2002 121 129
19. H.H. Lee, and L.K. Miller Isolation of genotypic variants of Autographa californica nuclear polyhedrosis virus J. Virol. 27 1978 754 767