A molecularly engineered split reporter for imaging protein-protein interactions with positron emission tomography

Nature Medicine

Volumn 16, Issue 8, 2010, Pages 921-926

  Massoud, Tarik F ^a,b,c   Paulmurugan, Ramasamy ^b,c   Gambhir, Sanjiv S ^b,c  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^b STANFORD UNIVERSITY   (United States)

^c STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALANINE; BINDING PROTEIN; CYSTEINE; ESTROGEN RECEPTOR; FKBP12 PROTEIN; HYPOXIA INDUCIBLE FACTOR 1ALPHA; THREONINE; THYMIDINE KINASE; UNCLASSIFIED DRUG; VALINE; VON HIPPEL LINDAU PROTEIN;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; GENETIC COMPLEMENTATION; HERPES SIMPLEX VIRUS 1; MODULATION; MOUSE; NONHUMAN; POINT MUTATION; POSITRON EMISSION TOMOGRAPHY; PRIORITY JOURNAL; PROTEIN ENGINEERING; PROTEIN FOLDING; PROTEIN PROTEIN INTERACTION; QUANTITATIVE ANALYSIS;

ANIMALS; CELLS, CULTURED; CLONING, MOLECULAR; GENES, REPORTER; HUMANS; HYPOXIA-INDUCIBLE FACTOR 1, ALPHA SUBUNIT; MICE; MODELS, BIOLOGICAL; PEPTIDE FRAGMENTS; POINT MUTATION; POSITRON-EMISSION TOMOGRAPHY; PROTEIN BINDING; PROTEIN ENGINEERING; PROTEIN INTERACTION MAPPING; TACROLIMUS BINDING PROTEIN 1A; TACROLIMUS BINDING PROTEINS; THYMIDINE KINASE; TRANSPLANTATION, HETEROLOGOUS; VON HIPPEL-LINDAU TUMOR SUPPRESSOR PROTEIN;

ANIMALIA; HUMAN HERPESVIRUS 1; MAMMALIA; MUS;

EID: 77955417371     PISSN: 10788956     EISSN: 1546170X     Source Type: Journal    
DOI: 10.1038/nm.2185     Document Type: Article

References (27)

1. Valdar, W.S. & Thornton, J.M. Protein-protein interfaces: analysis of amino acid conservation in homodimers. Proteins 42, 108-124 (2001).
2. Shoemaker, B.A. & Panchenko, A.R. Deciphering protein-protein interactions. Part I. Experimental techniques and databases. PLoS Comput. Biol. 3, e42 (2007).
3. Massoud, T.F. & Gambhir, S.S. Molecular imaging in living subjects: seeing fundamental biological processes in a new light. Genes Dev. 17, 545-580 (2003).
4. Michnick, S.W. Exploring protein interactions by interaction-induced folding of proteins from complementary peptide fragments. Curr. Opin. Struct. Biol. 11, 472-477 (2001).
5. Hu, C.D. & Kerppola, T.K. Simultaneous visualization of multiple protein interactions in living cells using multicolor fluorescence complementation analysis. Nat. Biotechnol. 21, 539-545 (2003).
6. Michnick, S.W., Remy, I., Campbell-Valois, F.X., Vallee-Belisle, A. & Pelletier, J.N. Detection of protein-protein interactions by protein fragment complementation strategies. Methods Enzymol. 328, 208-230 (2000).
7. Park, K. et al. A split enhanced green fluorescent protein-based reporter in yeast two-hybrid system. Protein J. 26, 107-116 (2007).
8. Zhang, S., Ma, C. & Chalfie, M. Combinatorial marking of cells and organelles with reconstituted fluorescent proteins. Cell 119, 137-144 (2004).
9. Paulmurugan, R., Umezawa, Y. & Gambhir, S.S. Noninvasive imaging of proteinprotein interactions in living subjects by using reporter protein complementation and reconstitution strategies. Proc. Natl. Acad. Sci. USA 99, 15608-15613 (2002).
10. Paulmurugan, R. & Gambhir, S.S. Monitoring protein-protein interactions using split synthetic Renilla luciferase protein fragment-assisted complementation. Anal. Chem. 75, 1584-1589 (2003).
11. Tjuvajev, J.G. et al. Imaging the expression of transfected genes in vivo. Cancer Res. 55, 6126-6132 (1995).
12. Kang, K.W., Min, J.J., Chen, X. & Gambhir, S.S. Comparison of [14C]FMAU, [3H]FEAU, [14C]FIAU, and [3H]PCV for monitoring reporter gene expression of wild type and mutant herpes simplex virus type 1 thymidine kinase in cell culture. Mol. Imaging Biol. 7, 296-303 (2005).
13. Iyer, M. et al. 8-[18F]Fluoropenciclovir: an improved reporter probe for imaging HSV1-tk reporter gene expression in vivo using PET. J. Nucl. Med. 42, 96-105 (2001).
14. Gambhir, S.S. et al. A mutant herpes simplex virus type 1 thymidine kinase reporter gene shows improved sensitivity for imaging reporter gene expression with positron emission tomography. Proc. Natl. Acad. Sci. USA 97, 2785-2790 (2000).
15. Massoud, T.F. Novel Approaches to Molecular Imaging of Protein-Protein Interactions in Living Subjects. PhD thesis, Univ. Cambridge (2007).
16. Spotts, J.M., Dolmetsch, R.E. & Greenberg, M.E. Time-lapse imaging of a dynamic phosphorylation-dependent protein-protein interaction in mammalian cells. Proc. Natl. Acad. Sci. USA 99, 15142-15147 (2002).
17. Chen, J. & Schreiber, S.L. Identification of an 11-kDa FKBP12-rapamycin-binding domain within the 289-kDa FKBP12-rapamycin-associated protein and characterization of a critical serine residue. Proc. Natl. Acad. Sci. USA 92, 4947-4951 (1995).
18. Remy, I. & Michnick, S.W. Clonal selection and in vivo quantitation of protein interactions with protein-fragment complementation assays. Proc. Natl. Acad. Sci. USA 96, 5394-5399 (1999).
19. Wurth, C., Thomas, R.M., Folkers, G. & Scapozza, L. Folding and self-assembly of herpes simplex virus type 1 thymidine kinase. J. Mol. Biol. 313, 657-670 (2001).
20. Jaakkola, P. et al. Targeting HIF-to the von Hippel-Lindau ubiquitylation complex by O2-regulated prolyl hydroxylation. Science 292, 468-472 (2001).
21. Paulmurugan, R. & Gambhir, S.S. An intramolecular folding sensor for imaging estrogen receptor-ligand interactions. Proc. Natl. Acad. Sci. USA 103, 15883-15888 (2006).
22. Peñuelas, I. et al. Positron emission tomography imaging of adenoviral-mediated transgene expression in liver cancer patients. Gastroenterology 128, 1787-1795 (2005).
23. Brannigan, J.A. & Wilkinson, A.J. Protein engineering 20 years on. Nat. Rev. Mol. Cell Biol. 3, 964-970 (2002).
24. Chen, R. Enzyme engineering: rational redesign versus directed evolution. Trends Biotechnol. 19, 13-14 (2001).
25. Massoud, T.F. & Gambhir, S.S. Integrating noninvasive molecular imaging into molecular medicine: an evolving paradigm. Trends Mol. Med. 13, 183-191 (2007).
26. Michnick, S.W., Ear, P.H., Manderson, E.N., Remy, I. & Stefan, E. Universal strategies in research and drug discovery based on protein-fragment complementation assays. Nat. Rev. Drug Discov. 6, 569-582 (2007).
27. Qi, J., Leahy, R.M., Cherry, S.R., Chatziioannou, A. & Farquhar, T.H. High-resolution 3D Bayesian image reconstruction using the microPET small-animal scanner. Phys. Med. Biol. 43, 1001-1013 (1998).