Expression and purification of untagged and histidine-tagged folate-dependent tRNA:m5U54 methyltransferase from Bacillus subtilis

Protein Expression and Purification

Volumn 73, Issue 1, 2010, Pages 83-89

  Hamdane, Djemel ^a   Skouloubris, Stèphane ^b,c   Myllykallio, Hannu ^d   Golinelli Pimpaneau, Bèatrice ^a  

^a LABORATOIRE D ENZYMOLOGIE ET BIOCHIMIE STRUCTURALES   (France)

^b UNIVERSITÉ PARIS SACLAY   (France)

^c CNRS   (France)

^d LABORATOIRE D OPTIQUE ET BIOSCIENCES   (France)

Author keywords

5 Methyluridine; Expression; Flavoenzyme; Histidine tag; Purification; Recombinant TrmFO protein; RNA methyltransferase

Indexed keywords

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 77955415071     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2010.04.013     Document Type: Article

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