Expression of cloned cDNA for the human mitochondrial RNA polymerase in Escherichia coli and purification

Protein Expression and Purification

Volumn 21, Issue 3, 2001, Pages 485-491

  Nam, Sang Chul ^a   Kang, Changwon ^a  

^a Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

Author keywords

Histidine tagged protein; Human mitochondrial RNA polymerase; In vitro transcription; Mitochondrial transcription factor A; Soluble expression of cloned cDNA

Indexed keywords

ESCHERICHIA COLI; MAMMALIA;

EID: 0035718956     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2000.1383     Document Type: Article

References (30)

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5. Mutations in the genes for mitochondrial RNA polymerase and a second mitochondrial transcription factor of Saccharomyces cerevisiae
6. The yeast mitochondrial RNA polymerase specificity factor, MTF1, is similar to bacterial sigma factors
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8. Release of the yeast mitochondrial RNA polymerase specificity factor from transcription complexes
9. A Saccharomyces cerevisiae mitochondrial transcription factor, sc-mtTFB, shares features with sigma factors but is functionally distinct
10. DNA binding properties of an HMG1-related protein from yeast mitochondria
11. A human mitochondrial transcriptional activator can functionally replace a yeast mitochondrial HMG box protein both in vivo and in vitro
12. Promoter selection in human mitochondria involves binding of a transcription factor to orientation-independent upstream regulatory elements
13. Flexible recognition of rapidly evolving promoter sequences by mitochondrial transcription factor 1
14. Similarity of human mitochondrial transcription factor 1 to high mobility group proteins
15. A single mouse gene encodes the mitochondrial transcription factor A and a testis-specific nuclear HMG-box protein
16. DNA wrapping and bending by a mitochondrial high mobility group-like transcriptional activator protein
17. Distinct roles for two purified factors in transcription of Xenopus mitochondrial DNA
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19. Identification of the gene encoding the human mitochondrial RNA polymerase (h-mtRPOL) by cyberscreening of the expressed sequence tags database
20. Human mitochondrial transcription factor A and promoter spacing integrity are required for transcription initiation
21. Impairment of mitochondrial transcription termination by a point mutation associated with the MELAS subgroup of mitochondrial encephalomyopathies
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24. In vitro transcription of human mitochondrial DNA: Identification of specific light strand transcripts from the displacement loop region
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26. Codon usage tabulated from international DNA sequence databases
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