Overexpression and affinity chromatography purification of the Type III restriction endonuclease EcoP15I for use in transcriptome analysis

Journal of Biotechnology

Volumn 114, Issue 1-2, 2004, Pages 99-106

  Möncke Buchner, Elisabeth ^a   Mackeldanz, Petra ^a   Krüger, Detlev H ^a   Reuter, Monika ^a  

^a CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

Author keywords

DNA sequence; EcoP15I operon; His tagged protein; pQE 16 Overexpression vector; Restriction endonuclease EcoP15I; SAGE; Transcriptome analysis

Indexed keywords

ENDONUCLEASES; GENE EXPRESSION; HEPARINS; PLASMIDS;

AFFINITY CHROMATOGRAPHY; BIOTECHNOLOGY; CELLS; DNA; GENES; ION EXCHANGE;

ENZYMES;

ENDONUCLEASE;

AFFINITY CHROMATOGRAPHY; ARTICLE; BINDING AFFINITY; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; DNA CLEAVAGE; DNA SEQUENCE; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE OVEREXPRESSION; ION EXCHANGE; MOLECULAR CLONING; MOLECULAR RECOGNITION; NONHUMAN; NUCLEOTIDE SEQUENCE; OPERON; PLASMID; PRIORITY JOURNAL; PURIFICATION;

AMINO ACID SEQUENCE; CHROMATOGRAPHY, AFFINITY; DNA RESTRICTION ENZYMES; ENZYME ACTIVATION; ESCHERICHIA COLI; GENE EXPRESSION PROFILING; MOLECULAR SEQUENCE DATA; PROTEIN ENGINEERING; PROTEOME; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, DNA; SITE-SPECIFIC DNA-METHYLTRANSFERASE (ADENINE-SPECIFIC); TRANSCRIPTION FACTORS;

EID: 4644272663     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2004.06.014     Document Type: Article

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