The importance of tryptic-like activity in purified enzyme blends for efficient islet isolation

Transplantation

Volumn 87, Issue 3, 2009, Pages 370-375

  Brandhorst, Heide ^a   Friberg, Andrew ^a   Andersson, Helena H ^b   Felldin, Maria ^c   Foss, A ^d   Salmela, Kaija ^e   Lundgren, Torbjörn ^f   Tibell, Annika ^f   Tufveson, Gunnar ^a   Korsgren, Olle ^a   Brandhorst, Daniel ^a  

^a UPPSALA UNIVERSITY HOSPITAL   (Sweden)

^b University Hospital   (Sweden)

^c SAHLGRENSKA UNIVERSITY HOSPITAL   (Sweden)

^d OSLO UNIVERSITY HOSPITAL   (Norway)

^e UNIVERSITY OF HELSINKI   (Finland)

^f KAROLINSKA INSTITUTE   (Sweden)

Author keywords

Collagenase; Human; Islet isolation; Rat; Tryptic like activity

Indexed keywords

BACTERIAL PROTEIN; COLLAGENASE; INSULIN; THERMOLYSIN; TRYPSIN;

ADULT; AGED; ANIMAL TISSUE; ARTICLE; CLOSTRIDIUM HISTOLYTICUM; DIABETES MELLITUS; ENZYME ACTIVITY; FEMALE; HUMAN; HUMAN TISSUE; ISOLATION PROCEDURE; MAJOR CLINICAL STUDY; MALE; NONHUMAN; OUTCOME ASSESSMENT; PANCREAS ISLET; PRIORITY JOURNAL; RAT; TISSUE CULTURE; ANIMAL; CELL SEPARATION; CELL SURVIVAL; CYTOLOGY; DONOR; EXPERIMENTAL DIABETES MELLITUS; GRAFT SURVIVAL; LEWIS RAT; METABOLISM; METHODOLOGY; MIDDLE AGED; MOUSE; NUDE MOUSE; PANCREAS ISLET BETA CELL; PANCREAS ISLET TRANSPLANTATION; PHYSIOLOGY; XENOGRAFT;

ANIMALS; CELL SEPARATION; CELL SURVIVAL; COLLAGENASES; DIABETES MELLITUS, EXPERIMENTAL; FEMALE; GRAFT SURVIVAL; HUMANS; INSULIN; INSULIN-SECRETING CELLS; ISLETS OF LANGERHANS; ISLETS OF LANGERHANS TRANSPLANTATION; MALE; MICE; MICE, NUDE; MIDDLE AGED; RATS; RATS, INBRED LEW; THERMOLYSIN; TISSUE DONORS; TRANSPLANTATION, HETEROLOGOUS; TRYPSIN;

EID: 61849174264     PISSN: 00411337     EISSN: None     Source Type: Journal    
DOI: 10.1097/TP.0b013e31819499f0     Document Type: Article

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