Embryonic in vivo electroporation in the mouse

Methods in Enzymology

Volumn 477, Issue C, 2010, Pages 37-50

  Saito, Tetsuichiro ^a  

^a CHIBA UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

NUCLEIC ACID;

ARTICLE; BRAIN VENTRICLE; CENTRAL NERVOUS SYSTEM; ELECTRODE; ELECTROPORATION; EMBRYO; EMBRYO DEVELOPMENT; GENE EXPRESSION; GENE TRANSFER; GENETIC TRANSFECTION; MOUSE; NERVOUS SYSTEM DEVELOPMENT; NONHUMAN; PREGNANCY; PRIORITY JOURNAL;

EID: 77955383748     PISSN: 00766879     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S0076-6879(10)77003-8     Document Type: Chapter

References (18)

1. Chen C., Smye S.W., Robinson M.P., Evans J.A. Membrane electroporation theories: A review. Med. Biol. Eng. Comput. 2006, 44:5-14.
2. Escoffre J.-M., Portet T., Wasungu L., Teissie J., Dean D., Rols M.-P. What is (still not) known of the mechanism by which electroporation mediates gene transfer and expression in cells and tissues. Mol. Biotechnol. 2009, 41:286-295.
3. Goldstein S., Fordis C.M., Howard B.H. Enhanced transfection efficiency and improved cell survival after electroporation of G2/M-synchronized cells and treatment with sodium butyrate. Nucleic Acids Res. 1989, 17:3959-3971.
4. Huang Z., Tamura M., Sakurai T., Chuma S., Saito T., Nakatsuji N. In vivo transfection of testicular germ cells and transgenesis by using the mitochondrially localized jellyfish fluorescent protein gene. FEBS Lett. 2000, 487:248-251.
5. Kaufman M.H. The Atlas of Mouse Development 1992, Academic Press.
6. Kawauchi D., Saito T. Transcriptional cascade from Math1 to Mbh1 and Mbh2 is required for cerebellar granule cell differentiation. Dev. Biol. 2008, 322:345-354.
7. Kawauchi D., Taniguchi H., Watanabe H., Saito T., Murakami F. Direct visualization of nucleogenesis by precerebellar neurons: Involvement of ventricle-directed, radial fibre-associated migration. Development 2006, 133:1113-1123.
8. Miyagi S., Saito T., Mizutani K., Masuyama N., Gotoh Y., Iwama A., Nakauchi H., Masui S., Niwa H., Nishimoto M., Muramatsu M., Okuda A. The Sox-2 regulatory regions display their activities in two distinct types of multipotent stem cells. Mol. Cell. Biol. 2004, 24:4207-4220.
9. Mizutani K., Saito T. Progenitors resume generating neurons after temporary inhibition of neurogenesis by Notch activation in the mammalian cerebral cortex. Development 2005, 132:1295-1304.
10. Muramatsu T., Mizutani Y., Ohmori Y., Okumura J. Comparison of three nonviral transfection methods for foreign gene expression in early chicken embryos in ovo. Biochem. Biophys. Res. Commun. 1997, 230:376-380.
11. Muroyama Y., Saito T. Identification of Nepro, a gene required for the maintenance of neocortex neural progenitor cells downstream of Notch. Development 2009, 136:3889-3893.
12. Neumann E., Schaefer-Ridder M., Wang Y., Hofschneider P.H. Gene transfer into mouse lyoma cells by electroporation in high electric fields. EMBO J. 1982, 1:841-845.
13. Saba R., Nakatsuji N., Saito T. Mammalian BarH1 confers commissural neuron identity on dorsal cells in the spinal cord. J. Neurosci. 2003, 23:1987-1991.
14. Saba R., Johnson J.E., Saito T. Commissural neuron identity is specified by a homeodomain protein, Mbh1, that is directly downstream of Math1. Development 2005, 132:2147-2155.
15. Saito T. Analysis of mammalian neuronal diversity using in vivo electroporation 1999, 1.
16. Saito T. In vivo electroporation in the embryonic mouse central nervous system. Nat. Protoc. 2006, 1:1552-1558.
17. Saito T., Nakatsuji N. Efficient gene transfer into the embryonic mouse brain using in vivo electroporation. Dev. Biol. 2001, 240:237-246.
18. Takahashi M., Furukawa T., Saitoh H., Aoki A., Koike T., Moriyama Y., Shibata A. Gene transfer into human leukemia cell lines by electroporation: Experience with exponentially decaying and square wave pulse. Leuk. Res. 1991, 15:507-513.