Serum-free scaled up expansion and differentiation of murine embryonic stem cells to osteoblasts in suspension bioreactors

Biotechnology and Bioengineering

Volumn 106, Issue 5, 2010, Pages 829-840

  Alfred, Roz ^a   Gareau, Tia ^a   Krawetz, Roman ^a   Rancourt, Derrick ^a   Kallos, Michael S ^a  

^a UNIVERSITY OF CALGARY   (Canada)

Author keywords

Embryonic stem cells; Osteoblasts; Serum free cultivation; Suspension bioreactor

Indexed keywords

BIOCONVERSION; BIOREACTORS; BODY FLUIDS; CALCIUM; CELL CULTURE; CELL GROWTH; EXPANSION; GENE TRANSFER; OSTEOBLASTS;

CALCIUM DEPOSITION; CLINICAL APPLICATION; CULTURE MEDIA; DEGENERATIVE DISEASE; EMBRYONIC STEM CELLS; FETAL BOVINE SERUM; MURINE EMBRYONIC STEM CELLS; MUSCULOSKELETAL DISEASE; OSTEOCALCIN; OSTEOGENIC CELLS; OSTERIX; SCALABLE PRODUCTION; SERUM-FREE CULTIVATION; SERUM-FREE MEDIA; STATIC CULTURE; UP-REGULATION;

STEM CELLS;

CALCIUM; OSTEOCALCIN; CULTURE MEDIUM;

ANIMAL CELL; ARTICLE; BIOREACTOR; CELL DIFFERENTIATION; EMBRYONIC STEM CELL; MESENCHYMAL STEM CELL; MINERALIZATION; MOUSE; NONHUMAN; OSTEOBLAST; STEM CELL EXPANSION; UPREGULATION; ANIMAL; CULTURE MEDIUM; CULTURE TECHNIQUE; CYTOLOGY;

ANIMALS; BIOREACTORS; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CULTURE MEDIA, SERUM-FREE; EMBRYONIC STEM CELLS; MICE; OSTEOBLASTS;

EID: 77955358863     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22727     Document Type: Article

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