Cultivation of human embryonic stem cells without the embryoid body step enhances osteogenesis in vitro

Stem Cells

Volumn 24, Issue 4, 2006, Pages 835-843

  Karp, Jeffrey M ^a   Ferreira, Lino S ^a,c   Khademhosseini, Ali ^b,d   Kwon, Albert H ^b   Yeh, Judy ^a   Langer, Robert S ^a,b  

^a Massachusetts Institute of Technology Cambridge   (United States)

^b MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^c INEB INSTITUTO DE ENGENHARIA BIOMÉDICA   (Portugal)

^d BRIGHAM AND WOMEN S HOSPITAL   (United States)

Author keywords

Bone tissue engineering; Embryoid bodies; Human embryonic stem cells; Matrix characterization; Osteogenic cell frequency

Indexed keywords

APATITE; CALCIUM; COLLAGEN; DEXAMETHASONE; HYDROXYAPATITE; PHOSPHORUS; ALKALINE PHOSPHATASE; CALCIUM PHOSPHATE; OSTEOCALCIN;

ARTICLE; BONE DEVELOPMENT; BONE GROWTH; BONE MATRIX; BONE MINERALIZATION; CELL DIFFERENTIATION; COLLAGEN SYNTHESIS; EMBRYO; EMBRYO CULTURE; EMBRYONIC STEM CELL; EXTRACELLULAR MATRIX; HUMAN; HUMAN CELL; HUMAN EMBRYO; IN VITRO STUDY; OSSIFICATION; SPHEROID CELL; TISSUE ENGINEERING; CELL SEPARATION; CULTURE TECHNIQUE; CYTOLOGY; METABOLISM; METHODOLOGY; TOTIPOTENT STEM CELL;

ALKALINE PHOSPHATASE; BONE MATRIX; CALCIUM PHOSPHATES; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELL SEPARATION; COLLAGEN; HUMANS; OSTEOCALCIN; OSTEOGENESIS; TISSUE ENGINEERING; TOTIPOTENT STEM CELLS;

EID: 33745504422     PISSN: 10665099     EISSN: None     Source Type: Journal    
DOI: 10.1634/stemcells.2005-0383     Document Type: Article

References (36)

1. Langer R, Vacanti JP. Tissue engineering. Science 1993;260:920-926.
2. Alsberg E, Anderson KW, Albeiruti A et al. Cell-interactive alginate hydrogels for bone tissue engineering. J Dent Res 2001;80:2025-2029.
3. Bhatia M. Derivation of the hematopoietic stem cell compartment from human embryonic stem cell lines. Ann N Y Acad Sci 2005;1044:24-28.
4. Thomson JA, Itskovitz-Eldor J, Shapiro SS et al. Embryonic stem cell lines derived from human blastocysts. Science 1998;282:1145-1147 [erratum in Science 1998 282:1827].
5. Perlingeiro RC, Kyba M, Daley GQ. Clonal analysis of differentiating embryonic stem cells reveals a hematopoietic progenitor with primitive erythroid and adult lymphoid-myeloid potential. Development 2001;128:4597-4604.
6. zur Nieden NI, Kempka G, Ahr HJ. In vitro differentiation of embryonic stem cells into mineralized osteoblasts. Differentiation 2003;71:18-27.
7. Bourne S, Polak JM, Hughes SP et al. Osteogenic differentiation of mouse embryonic stem cells: Differential gene expression analysis by cDNA microarray and purification of osteoblasts by cadherin-11 magnetically activated cell sorting. Tissue Eng 2004;10:796-806.
8. Buttery LD, Bourne S, Xynos JD et al. Differentiation of osteoblasts and in vitro bone formation from murine embryonic stem cells. Tissue Eng 2001;7:89-99.
9. Shimko DA, Burks CA, Dee KC et al. Comparison of in vitro mineralization by murine embryonic and adult stem cells cultured in an osteogenic medium. Tissue Eng 2004;10:1386-1398.
10. Bronson SK. Bone nodule formation via in vitro differentiation of murine embryonic stem cells. Methods Enzymol 2003;365:241-251.
11. Sottile V, Thomson A, McWhir J. In vitro osteogenic differentiation of human ES cells. Cloning Stem Cells 2003;5:149-155.
12. Bielby RC, Boccaccini AR, Polak JM et al. In vitro differentiation and in vivo mineralization of osteogenic cells derived from human embryonic stem cells. Tissue Eng 2004;10:1518-1525.
13. Cao T, Heng BC, Ye CP et al. Osteogenic differentiation within intact human embryoid bodies result in a marked increase in osteocalcin secretion after 12 days of in vitro culture, and formation of morphologically distinct nodule-like structures. Tissue Cell 2005;37:325-334.
14. Ginis I, Luo Y, Miura T et al. Differences between human and mouse embryonic stem cells. Dev Biol 2004;269:360-380.
15. Itskovitz-Eldor J, Schuldiner M, Karsenti D et al. Differentiation of human embryonic stem cells into embryoid bodies compromising the three embryonic germ layers. Mol Med 2000;6:88-95.
16. Keller GM. In vitro differentiation of embryonic stem cells. Curr Opin Cell Biol 1995;7:862-869.
17. Yamada T, Yoshikawa M, Kanda S et al. In vitro differentiation of embryonic stem cells into hepatocyte-like cells identified by cellular uptake of indocyanine green. STEM CELLS 2002;20:146-154.
18. Aubin JE. Osteoprogenitor cell frequency in rat bone marrow stromal populations: Role for heterotypic cell-cell interactions in osteoblast differentiation. J Cell Biochem 1999;72:396-410.
19. Sarugaser R, Lickorish D, Baksh D et al. Human umbilical cord perivascular (HUCPV) cells: A source of mesenchymal progenitors. STEM CELLS 2005;23:220-229.
20. Komori T, Yagi H, Nomura S et al. Targeted disruption of Cbfa1 results in a complete lack of bone formation owing to maturational arrest of osteoblasts. Cell 1997;89:755-764.
21. Bonewald LF, Harris SE, Rosser J et al. von Kossa staining alone is not sufficient to confirm that mineralization in vitro represents bone formation. Calcif Tissue Int 2003;72:537-547.
22. Amit M, Carpenter MK, Inokuma MS et al. Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged periods of culture. Dev Biol 2000;227:271-278.
23. Karp JM, Shoichet MS, Davies JE. Bone formation on two-dimensional poly(DL-lactide-co-glycolide) (PLGA) films and three-dimensional PLGA tissue engineering scaffolds in vitro. J Biomed Mater Res 2003;64A:388-396.
24. Maniatopoulos C, Sodek J, Melcher AH. Bone formation in vitro by stromal cells obtained from bone marrow of young adult rats. Cell Tissue Res 1988;254:317-330.
25. Davies JE. In vitro modeling of the bone/implant interface. Anat Rec 1996;245:426-445.
26. Slizova D, Krs O, Pospisilova B. Alternative method of rapid drying vascular specimens for scanning electron microscopy. J Endovasc Ther 2003;10:285-287.
27. Purpura KA, Aubin JE, Zandstra PW. Two-color image analysis discriminates between mineralized and unmineralized bone nodules in vitro. Biotechniques 2003;34:1188-1192, 1194, 1196 passim.
28. Purpura KA, Aubin JE, Zandstra PW. Sustained in vitro expansion of bone progenitors is cell density dependent. STEM CELLS 2004;22:39-50.
29. Klimanskaya I, Chung Y, Meisner L et al. Human embryonic stem cells derived without feeder cells. Lancet 2005;365:1636-1641.
30. Draper JS, Pigott C, Thomson JA et al. Surface antigens of human embryonic stem cells: Changes upon differentiation in culture. J Anat 2002;200:249-258.
31. Aubin JE, Liu F, Malaval L et al. Osteoblast and chondroblast differentiation. Bone 1995;17:77S-83S.
32. Aubin JE. Bone stem cells. J Cell Biochem Suppl 1998;30-31:73-82.
33. Schmitt B, Ringe J, Haupl T et al. BMP2 initiates chondrogenic lineage development of adult human mesenchymal stem cells in high-density culture. Differentiation 2003;71:567-577.
34. Lian JB, McKee MD, Todd AM et al. Induction of bone-related proteins, osteocalcin and osteopontin, and their matrix ultrastructural localization with development of chondrocyte hypertrophy in vitro. J Cell Biochem 1993;52:206-219.
35. Wei CL, Miura T, Robson P et al. Transcriptome profiling of human and murine ESCs identifies divergent paths required to maintain the stem cell state. STEM CELLS 2005;23:166-185.
36. Heng BC, Cao T, Stanton LW et al. Strategies for directing the differentiation of stem cells into the osteogenic lineage in vitro. J Bone Miner Res 2004;19:1379-1394.