Embryonic stem cells remain highly pluripotent following long term expansion as aggregates in suspension bioreactors

Journal of Biotechnology

Volumn 129, Issue 3, 2007, Pages 421-432

  zur Nieden, Nicole I ^a   Cormier, Jaymi T ^a   Rancourt, Derrick E ^a   Kallos, Michael S ^a  

^a UNIVERSITY OF CALGARY   (Canada)

Author keywords

Embryoid body formation; Embryonic stem cells; Long term culture; Pluripotency; Suspension bioreactor

Indexed keywords

ANIMAL CELL CULTURE; BIOMARKERS; BIOREACTORS; GENE EXPRESSION; OSTEOBLASTS; SUSPENSIONS (FLUIDS); TISSUE;

BIOREACTOR PROTOCOLS; CARDIOMYOCYTES; CHONDROCYTES; EMBRYOID BODY (EB); EMBRYONIC STEM CELLS (ESC); PLURIPOTENCY; SUSPENSION BIOREACTORS;

CELL GROWTH;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; BIOREACTOR; CARTILAGE CELL; CELL AGGREGATION; CELL CULTURE; CELL DIFFERENTIATION; CELL LINEAGE; COLONY FORMATION; CONTROLLED STUDY; CULTURE TECHNIQUE; EMBRYONIC STEM CELL; FLOW CYTOMETRY; GENE EXPRESSION PROFILING; HEART MUSCLE CELL; MOUSE; NERVE CELL; NONHUMAN; OSTEOBLAST; PLURIPOTENT STEM CELL; PRIORITY JOURNAL; SUSPENSION BIOREACTOR; TERATOMA;

ANIMALS; BIOREACTORS; CALCIUM; CELL DIFFERENTIATION; CELL PROLIFERATION; DNA PRIMERS; EMBRYONIC STEM CELLS; FLOW CYTOMETRY; FLUORESCENT ANTIBODY TECHNIQUE; MICE; OXYGEN; PLURIPOTENT STEM CELLS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; TISSUE ENGINEERING;

MURINAE;

EID: 34147145008     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2007.01.006     Document Type: Article

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