Detection and quantification of Toxoplasma gondii in ovine maternal and foetal tissues from experimentally infected pregnant ewes using real-time PCR

Veterinary Parasitology

Volumn 172, Issue 1-2, 2010, Pages 8-15

  Gutierrez, J ^a   O'Donovan, J ^b   Williams, E ^a   Proctor, A ^a   Brady, C ^b   Marques, P X ^a   Worrall, S ^a   Nally, J E ^a   McElroy, M ^b   Bassett, H ^a   Sammin, D ^b   Buxton, D ^c   Maley, S ^c   Markey, B K ^a  

^a UNIVERSITY COLLEGE DUBLIN   (Ireland)

^b CENTRAL VETERINARY RESEARCH LABORATORY   (Ireland)

^c MOREDUN RESEARCH INSTITUTE   (United Kingdom)

Author keywords

Ovine abortion; Real time PCR; Toxoplasma gondii

Indexed keywords

DNA;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; DNA DETERMINATION; EWE; FEMALE; FETUS; HISTOPATHOLOGY; LIVER; LYMPH NODE; NONHUMAN; PARASITE IDENTIFICATION; PARASITE LOCALIZATION; PREGNANCY; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; SPLEEN; TOXOPLASMA GONDII; TOXOPLASMOSIS; UTERUS;

ANIMALS; BRAIN; DNA, PROTOZOAN; FEMALE; FETUS; IMMUNOHISTOCHEMISTRY; PLACENTA; POLYMERASE CHAIN REACTION; PREGNANCY; RANDOM ALLOCATION; SHEEP; SHEEP DISEASES; TOXOPLASMA; TOXOPLASMOSIS, ANIMAL;

OVIS; TOXOPLASMA; TOXOPLASMA GONDII;

EID: 77955321703     PISSN: 03044017     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.vetpar.2010.04.035     Document Type: Article

References (40)

1. Bennet, R., Ijpelaar, J., 2003. Economic assessment of livestock diseases in Britain. Final Reports to the DEFRA, 2003, 30 pp., September.
2. Burg J.L., Grover C.M., Pouletty P., Boothroyd J.C. Direct and sensitive detection of a pathogenic protozoan, Toxoplasma gondii, by polymerase chain reaction. J. Clin. Microbiol. 1989, 27:1787-1792.
3. Buxton D. Protozoan infections (Toxoplasma gondii, Neospora caninum and Sarcocystis spp.) in sheep and goats: recent advances. Vet. Res. 1998, 29:289-310.
4. Buxton D., Finlayson J. Experimental infection of pregnant sheep with Toxoplasma gondii: pathological and immunological observations on the placenta and foetus. J. Comp. Pathol. 1986, 96:319-333.
5. Buxton D., Thomson K., Maley S., Wright S., Bos H.J. Vaccination of sheep with a live incomplete strain (S48) of Toxoplasma gondii and their immunity to challenge when pregnant. Vet. Rec. 1991, 129:89-93.
6. Buxton D., Thomson K.M., Maley S., Wright S., Bos H.J. Experimental challenge of sheep 18 months after vaccination with a live (S48) Toxoplasma gondii vaccine. Vet. Rec. 1993, 133:310-312.
7. Buxton D., Thomson K.M., Maley S. Treatment of ovine toxoplasmosis with a combination of sulphamezathine and pyrimethamine. Vet. Rec. 1993, 132:409-411.
8. Buxton D., Brebner J., Wright S., Maley S.W., Thomson K.M., Millard K. Decoquinate and the control of experimental ovine toxoplasmosis. Vet. Rec. 1996, 138:434-436.
9. Buxton D., Maley S.W., Wright S.E., Rodger S., Bartley P., Innes E.A. Toxoplasma gondii and ovine toxoplasmosis: new aspects of an old story. Vet. Parasitol. 2007, 149:25-28.
10. Cassaing S., Bessieres M.H., Berry A., Berrebi A., Fabre R., Magnaval J.F. Comparison between two amplification sets for molecular diagnosis of toxoplasmosis by real-time PCR. J. Clin. Microbiol. 2006, 44(3):720-724.
11. Coughlan S.N., Saman E., Jacobs D., Mercier C., Cesbron-Delauw M.F., Trees A.J. Cellular and humoral immune responses to recombinant antigens in sheep infected with Toxoplasma gondii. Parasite Immunol. 1995, 17:465-468.
12. Dubey J.P. Toxoplasmosis in sheep-the last 20 years. Vet. Parasitol. 2009, 163:1-14.
13. Dubey J.P., Emond J.P., Desmonts G., Anderson W.R. Serodiagnosis of postnatally and prenatally induced toxoplasmosis in sheep. Am. J. Vet. Res. 1987, 48:1239-1243.
14. Duncanson P., Terry R.S., Smith J.E., Hide G. High levels of congenital transmission of Toxoplasma gondii in a commercial flock. Int. J. Parasitol. 2001, 31:1699-1703.
15. Dworkin L.L., Gibler T.M., Van Gelder R.N. Real-time quantitative polymerase chain reaction diagnosis of infectious posterior uveitis. Arch. Ophthalmol. 2002, 120(11):1534-1539.
16. Edvinsson B., Lappalainen M., Evengård B. Real-time PCR targeting a 529-bp repeat element for diagnosis of toxoplasmosis. Clin. Microbiol. Infect. 2006, 12:131-136.
17. Esteban-Redondo I., Innes E.A. Detection of Toxoplasma gondii in tissues of sheep orally challenged with different doses of oocysts. Int. J. Parasitol. 1998, 28:1459-1466.
18. Esteban-Redondo I., Maley S.W., Thomson K., Nicoll S., Wright S., Buxton D., Innes E.A. Detection of T. gondii in tissues of sheep and cattle following oral infection. Vet. Parasitol. 1999, 86:155-171.
19. Fleck D.G., Kwantes W. The Laboratory Diagnosis of Toxoplasmosis. Monograph Series 13 1980, Public Health Laboratory Service, London. D.G. Fleck, W. Kwantes (Eds.).
20. Hoffmann B., Depner K., Schirrmeier H., Beer M. A universal heterologous internal control system for duplex real-time RT-PCR assays used in a detection system for pestiviruses. J. Virol. Methods 2006, 136:200-209.
21. Homan W.L., Vercammen M., De Braekeleer J., Verschueren H. Identification of a 200- to 300-fold repetitive 529bp DNA fragment in Toxoplasma gondii, and its use for diagnostic and quantitative PCR. Int. J. Parasitol. 2000, 30:69-75.
22. Hurtado A., Aduriz G., Moreno B., Barandika J., García-Pérez A.L. Single tube nested PCR for the detection of Toxoplasma gondii in foetal tissues from naturally aborted ewes. Vet. Parasitol. 2001, 102:17-27.
23. Kirkbride C.A., Dubey J.P., Libal M.C. Effect of feeding lasalocid to pregnant ewes experimentally infected with Toxoplasma gondii. Vet. Parasitol. 1992, 44:299-303.
24. Kupferschmidt O., Krüger D., Held T.K., Ellerbrok H., Siegert W., Janitschke K. Quantitative detection of Toxoplasma gondii DNA in human body fluids by TaqMan polymerase chain reaction. Clin. Microbiol. Infect. 2001, 7(3):120-124.
25. Lin M.H., Chen T.C., Kuo T.T., Tseng C.C., Tseng C.P. Real-time PCR for quantitative detection of Toxoplasma gondii. J. Clin. Microbiol. 2000, 38:4121-4125.
26. Masala G., Porcu R., Madau L., Tanda A., Ibba B., Satta G., Tola S. Survey of ovine and caprine toxoplasmosis by IFAT and PCR assays in Sardinia, Italy. Vet. Parasitol. 2003, 117:15-21.
27. Masala G., Porcu R., Daga C., Denti S., Canu G., Patta C., Tola S. Detection of pathogens in ovine and caprine abortion samples from Sardinia, Italy, by PCR. J. Vet. Diagn. Invest. 2007, 19(1):96-98.
28. McColgan C., Buxton D., Blewett D.A. Titration of Toxoplasma gondii oocysts in non-pregnant sheep and the effects of subsequent challenge during pregnancy. Vet. Rec. 1988, 123:467-470.
29. Nagy B., Bán Z., Beke A., Nagy G.R., Lázár L., Papp C., Tóth-Pál E., Papp Z. Detection of Toxoplasma gondii from amniotic fluid, a comparison of four different molecular biological methods. Clin. Chim. Acta 2006, 368:131-137.
30. Owen M.R., Clarkson M.J., Trees A.J. Diagnosis of Toxoplasma abortion in ewes by polymerase chain reaction. Vet. Rec. 1998, 25:445-448.
31. Owen M.R., Clarkson M.J., Trees A.J. Acute phase Toxoplasma abortions in sheep. Vet. Rec. 1998, 142:480-482.
32. Pereira-Bueno J., Quintanilla-Gozalo A., Pérez-Pérez V., Álvarez-García G., Collantes-Fernández E., Ortega-Mora L.M. Evaluation of ovine abortion associated with Toxoplasma gondii in Spain by different diagnostic techniques. Vet. Parasitol. 2004, 121:33-43.
33. Reischl U., Bretagne S., Krüger D., Ernault P., Costa J.M. Comparison of two DNA targets for the diagnosis of toxoplasmosis by real-time PCR using fluorescence resonance energy transfer hybridization probes. BMC Infect. Dis. 2003, 3:7.
34. Samad M.A., Clarkson M.J. Prophylactic efficacy of decoquinate in the control of experimentally induced toxoplasmosis in pregnant ewes. Bangladesh Vet. J. 1995, 29:43-50.
35. Sargison N. Toxoplasma Abortion in Sheep 2003, National Animal Disease Information Service Disease Bulletin, UK.
36. Sreekumar C., Rao J.R., Mishra A.K., Ray D., Joshi P., Singh R.K. Detection of toxoplasmosis in experimentally infected goats by PCR. Vet. Rec. 2004, 154(20):632-635.
37. Switaj K., Master A., Skrzypczak M., Zaborowski P. Recent trends in molecular diagnostics for Toxoplasma gondii infections. Clin. Microbiol. Infect. 2005, 11:170-176.
38. Tenter A.M., Heckeroth A.R., Weiss L.M. Toxoplasma gondii: from animals to humans. Int. J. Parasitol. 2000, 30:1217-1258.
39. Uggla A., Sjoland L., Dubey J.P. Immunohistochemical diagnosis of toxoplasmosis in fetuses and fetal membranes of sheep. Am. J. Vet. Res. 1987, 48:348-357.
40. Williams E.J., O'Donovan J. Ovine abortion: an overview. Irish Vet. J. 2009, 62(5):342-346.