Use of a colorimetric assay to measure differences in cytotoxicity of Mycobacterium tuberculosis strains

Journal of Medical Microbiology

Volumn 56, Issue 6, 2007, Pages 733-737

  Castro Garza, Jorge ^a   Barrios García, Hugo B ^a,b   Cruz Vega, Delia Elva ^a   Said Fernández, Salvador ^a   Carranza Rosales, Pilar ^a   Molina Torres, Carmen A ^c   Vera Cabrera, Lucio ^c  

^a INSTITUTO MEXICANO DEL SEGURO SOCIAL   (Mexico)

^b UNIVERSIDAD AUTÓNOMA DE NUEVO LEÓN   (Mexico)

^c HOSPITAL UNIVERSITARIO DR JOSÉ ELEUTERIO GONZÁLEZ   (Mexico)

Author keywords

[No Author keywords available]

Indexed keywords

CRYSTAL VIOLET; PHOSPHOLIPASE C;

ARTICLE; BACTERIAL STRAIN; CELL DESTRUCTION; CELL LINE; COLORIMETRY; CONTROLLED STUDY; CYTOTOXICITY; GENE LOCUS; HUMAN; HUMAN CELL; MICROSCOPY; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; QUANTITATIVE ASSAY; RELIABILITY; SENSITIVITY AND SPECIFICITY; TECHNIQUE;

BACTERIAL PROTEINS; BACTERIOLOGICAL TECHNIQUES; CELL LINE, TUMOR; CELL SURVIVAL; COLORIMETRY; GENE DELETION; GENTIAN VIOLET; HUMANS; MYCOBACTERIUM TUBERCULOSIS; PHOSPHOLIPASE C; SENSITIVITY AND SPECIFICITY;

EID: 34250338501     PISSN: 00222615     EISSN: None     Source Type: Journal    
DOI: 10.1099/jmm.0.46915-0     Document Type: Article

References (27)

1. Carter, G., Wu, M., Drummond, D. C. & Bermudez, L. E. (2003). Characterization of biofilm formation by clinical isolates of Mycobacterium avium. J Med Microbiol 52, 747-752.
2. Castro-Garza, J., Quinn, F. D. & King, C. H. (1997). Assay to assess the cytotoxic effect of Mycobacterium tuberculosis and Mycobacterium bovis BCG on human alveolar pneumocytes. Abstract no. A-50 in Abstracts of the ASM Conference on Tuberculosis: Past, Present and Future, 1997. Copper Mountain, CO: American Society for Microbiology.
3. Castro-Garza, J., King, C. H., Swords, W. E. & Quinn, F. D. (2002). Demonstration of spread by Mycobacterium tuberculosis bacilli in A549 epithelial cell monolayers. FEMS Microbiol Lett 212, 145-149.
4. Danelishvili, L., McGarvey, J., Li, Y. J. & Bermudez, L. E. (2003). Mycobacterium tuberculosis infection causes different levels of apoptosis and necrosis in human macrophages and alveolar epithelial cells. Cell Microbiol 5, 649-660.
5. Daniel, A. K., Lee, R. E., Portaels, F. & Small, P. L. (2004). Analysis of Mycobacterium species for the presence of a macrolide toxin, mycolactone. Infect Immun 72, 123-132.
6. Dobos, K. M., Spotts, E. A., Quinn, F. D. & King, C. H. (2000). Necrosis of lung epithelial cells during infection with Mycobacterium tuberculosis is preceded by cell permeation. Infect Immun 68, 6300-6310.
7. Ferrari, M., Chiara, F. M. & Isetta, A. M. (1990). MTT colorimetric assay for testing macrophage cytotoxic activity in vitro. J Immunol Methods 131, 165-172.
8. Fischer, L. J., Quinn, F. D., White, E. & King, C. H. (1996). Intracellular growth and cytotoxicity of Mycobacterium haemophilum in a human epithelial cell line (Hec-1-B). Infect Immun 64, 269-276.
9. Franzblau, S. G., Witzig, R. S., McLaughlin, J. C., Torres, P., Madico, G., Hernandez, A., Degnan, M. T., Cook, M. B., Quenzer, V. K. & other authors (1998). Rapid, low-technology MIC determination with clinical Mycobacterium tuberculosis isolates by using the microplate Alamar Blue assay. J Clin Microbiol 36, 362-366.
10. Gillies, R. J., Didier, N. & Denton, M. (1986). Determination of cell number in monolayer cultures. Anal Biochem 159, 109-113.
11. Gomez-Flores, R., Gupta, S., Tamez-Guerra, R. & Mehta, R. T. (1995). Determination of MICs for Mycobacterium avium-M. intracellulare complex in liquid medium by a colorimetric method. J Clin Microbiol 33, 1842-1846.
12. Harhaji, L., Vuckovic, O., Miljkovic, D., Stosic-Grujicic, S. & Trajkovic, V. (2004). Iron down-regulates macrophage anti-tumour activity by blocking nitric oxide production. Clin Exp Immunol 137, 109-116.
13. Ishiyama, M., Shiga, M., Sasamoto, K., Mizoguchi, M. & He, P. (1993). A new sulfonated tetrazolium salt that produces a highly water-soluble formazan dye. Chem Pharm Bull (Tokyo) 41, 1118-1122.
14. McDonough, K. A. & Kress, Y. (1995). Cytotoxicity for lung epithelial cells is a virulence-associated phenorype of Mycobacterium tuberculosis. Infect Immun 63, 4802-4811.
15. Petrini, B. (2006). Mycobacterium abscessus: an emerging rapid-growing potential pathogen. APMIS 114, 319-328.
16. Ranger, B. S., Mahrous, E. A., Mosi, L., Adusumilli, S., Lee, R. E., Colorni, A., Rhodes, M. & Small, P. L. (2006). Globally distributed mycobacterial fish pathogens produce a novel plasmid-encoded toxic macrolide, mycolactone F. Infect Immun 74, 6037-6045.
17. Raynaud, C., Guilhot, C., Rauzier, J., Bordat, Y., Pelicic, V., Manganelli, R., Smith, I., Gicquel, B. & Jackson, M. (2002). Phospholipases C are involved in the virulence of Mycobacterium tuberculosis. Mol Microbiol 45, 203-217.
18. Read, J. K., Heggie, C. M., Meyers, W. M. & Connor, D. H. (1974). Cytotoxic activity of Mycobacterium ulcerans. Infect Immun 9, 1114-1122.
19. Rothman, S. W. (1986). Technique for measuring 50 % end points in cytotoxicity assays for Clostridium difficile toxins. J Clin Pathol 39, 672-676.
20. Shaik, M. S., Chatterjee, A. & Singh, M. (2004). Effects of monensin liposomes on the cytotoxicity, apoptosis and expression of multidrug resistance genes in doxorubicin-resistant human breast tumour (MCF-7/dox) cell-line. J Pharm Pharmacol 56, 899-907.
21. Takii, T., Hamasaki, S., Hirano, K., Abe, C. & Onozaki, K. (2005). Simple fibroblast-based assay to test pyrazinamide susceptibility of Mycobacterium tuberculosis. Antimicrob Agents Chemother 49, 804-807.
22. Thomas, M., Finnegan, C. E., Rogers, K. M., Purcell, J. W., Trimble, A., Johnston, P. G. & Boland, M. P. (2004). STAT1: a modulator of chemotherapy-induced apoptosis. Cancer Res 64, 8357-8364.
23. Torrado, E., Fraga, A. G., Castro, A. G., Stragier, P., Meyers, W. M., Portaels, F., Silva, M. T. & Pedrosa, J. (2007). Evidence for an intramacrophage growth phase of Mycobacterium ulcerans. Infect Immun 75, 977-987.
24. Trifiro, S., Bourgault, A. M., Lebel, F. & Rene, P. (1990). Ghost mycobacteria on Gram stain. J Clin Microbiol 28, 146-147.
25. Trucksis, M., Pritchett, C. L. & Reimschuessel, R. (2005). Mycobacterium marinum and fish and frog models of infection. In Tuberculosis and the Tubercle Bacillus, pp. 529-535. Edited by S. T. Cole, K. D. Eisenach, D. N. McMurray & W. R. Jacobs, Jr. Washington, DC: American Society for Microbiology.
26. Vera-Cabrera, L., Molina-Torres, C., Hernandez-Vera, M. A., Barrios-Garcia, H. B., Balckwood, K., Villarreal-Treviño, L., Ocampo-Candiani, J., Welsh, O. & Castro-Garza, J. (2007). Genetic characterization of Mycobacterium tuberculosis clinical isolates with deletions in the plcA-plcB-plcC locus. Tuberculosis (Edinb) 87, 21-29.
27. Zivadinovic, D., Gametchu, B. & Watson, C. S. (2005). Membrane estrogen receptor-alpha levels in MCF-7 breast cancer cells predict cAMP and proliferation responses. Breast Cancer Res 7, R101-R112.