An optimized electrofusion-based protocol for generating virus-specific human monoclonal antibodies

Journal of Immunological Methods

Volumn 336, Issue 2, 2008, Pages 142-151

  Yu, Xiaocong ^a   McGraw, Patricia A ^a   House, Frances S ^a   Crowe Jr , James E ^a,b  

^a VANDERBILT UNIVERSITY MEDICAL CENTER   (United States)

^b VANDERBILT UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Antibodies; Humoral; Hybridomas; Immunity; Monoclonal

Indexed keywords

MACROGOL; MONOCLONAL ANTIBODY; OUABAIN;

ANIMAL CELL; ARTICLE; B LYMPHOCYTE; CANCER CELL CULTURE; CELL FUSION; CONTROLLED STUDY; CPG ISLAND; ELECTROFUSION (CELL); ENZYME LINKED IMMUNOSORBENT ASSAY; EPSTEIN BARR VIRUS; HUMAN; HUMAN CELL; HYBRIDOMA; INFLUENZA VIRUS A H3N2; MYELOMA CELL; NONHUMAN; PERIPHERAL BLOOD MONONUCLEAR CELL; PRIORITY JOURNAL; RESPIRATORY SYNCYTIAL PNEUMOVIRUS; VIRUS RESISTANCE; VIRUS STRAIN; VIRUS TRANSFORMATION;

AMINOPTERIN; ANTIBODIES, MONOCLONAL; ANTIBODIES, VIRAL; B-LYMPHOCYTES; CELL FUSION; CELL LINE, TUMOR; CELL TRANSFORMATION, VIRAL; COCULTURE TECHNIQUES; HERPESVIRUS 4, HUMAN; HUMANS; HYBRIDOMAS; HYPOXANTHINE; IMMUNOGLOBULIN G; INFLUENZA A VIRUS, H3N2 SUBTYPE; MULTIPLE MYELOMA; OLIGODEOXYRIBONUCLEOTIDES; OUABAIN; RESPIRATORY SYNCYTIAL VIRUS, HUMAN; THYMIDINE;

HUMAN HERPESVIRUS 4; RESPIRATORY SYNCYTIAL VIRUS;

EID: 46149113910     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2008.04.008     Document Type: Article

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