Use of collagen gel as a three-dimensional in vitro model to study electropermeabilization and gene electrotransfer

Journal of Membrane Biology

Volumn 236, Issue 1, 2010, Pages 87-95

  Haberl, Saša ^a   Pavlin, Mojca ^a  

^a UNIVERSITY OF LJUBLJANA   (Slovenia)

Author keywords

3 D in vitro model; CHO cell; Collagen gel; Electropermeabilization; Gene electrotransfer; GFP; Propidium iodide

Indexed keywords

COLLAGEN GEL; GREEN FLUORESCENT PROTEIN; PLASMID DNA;

ANIMAL CELL; ARTICLE; CELL CULTURE; CHO CELL; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; ELECTROPORATION; EX VIVO STUDY; GENE EXPRESSION; IN VITRO STUDY; IN VIVO STUDY; NONHUMAN; THREE DIMENSIONAL IMAGING;

ANIMALS; CHO CELLS; CRICETINAE; CRICETULUS; DNA; ELECTROPORATION; GENE TRANSFER TECHNIQUES; MODELS, BIOLOGICAL; PLASMIDS; RATS;

EID: 77955305935     PISSN: 00222631     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00232-010-9280-3     Document Type: Article

References (58)

1. H Aihara J Miyazaki 1998 Gene transfer into muscle by electroporation in vivo Nat Biotechnol 16 867 870 10.1038/nbt0998-867 1:CAS:528:DyaK1cXlvFWku7k%3D 9743122
2. F Andre J Gehl G Sersa, et al. 2008 Efficiency of high and low voltage pulse combinations for gene electrotransfer in muscle, liver, tumor and skin Hum Gene Ther 19 1261 1271 10.1089/hum.2008.060 1:CAS:528:DC%2BD1cXhsVKls7vK 19866490
3. GL Andreason GA Evans 1989 Optimization of electroporation for transfection of mammalian cell lines Anal Biochem 180 269 275 10.1016/0003-2697(89)90429-6 1:CAS:528:DyaL1MXlslSntrY%3D 2817356
4. JE Barralet L Wang M Lawson, et al. 2005 Comparison of bone marrow cell growth on 2D and 3D alginate hydrogels J Mater Sci Mater Med 16 515 519 10.1007/s10856-005-0526-z 1:CAS:528:DC%2BD2MXks1Cisr0%3D 15928866 (Pubitemid 40884243)
5. P Bernstein M Dong D Corbeil, et al. 2009 Pellet culture elicits superior chondrogenic redifferentiation than alginate-based systems Biotechnol Prog 25 1146 1152 10.1002/btpr.186 1:CAS:528:DC%2BD1MXht1Cjur7N 19572391
6. M Bettan MA Ivanov LM Mir, et al. 2000 Efficient DNA electrotransfer into tumors Bioelectrochemistry 52 83 90 10.1016/S0302-4598(00)00087-8 1:CAS:528:DC%2BD3cXmsl2kur4%3D 11059581
7. MF Bureau J Gehl V Deleuze, et al. 2000 Importance of association between permeabilization and electrophoretic forces for intramuscular DNA electrotransfer Biochim Biophys Acta 1474 353 359 1:CAS:528:DC%2BD3cXisFGlurw%3D 10779687
8. CM Byrne JF Thompson 2006 Role of electrochemotherapy in the treatment of metastatic melanoma and other metastatic and primary skin tumors Expert Rev Anticancer Ther 6 671 678 10.1586/14737140.6.5.671 1:CAS:528: DC%2BD28XlsFGmtrk%3D 16759159 (Pubitemid 44192884)
9. PJ Canatella MM Black DM Bonnichsen, et al. 2004 Tissue electroporation: quantification and analysis of heterogeneous transport in multicellular environments Biophys J 86 3260 3268 10.1016/S0006-3495(04)74374-X 1:CAS:528:DC%2BD2cXjvVyis7o%3D 15111439
10. M Cemazar G Sersa J Wilson, et al. 2002 Effective gene transfer to solid tumors using different nonviral gene delivery techniques: electroporation, liposomes, and integrin-targeted vector Cancer Gene Ther 9 399 406 10.1038/sj.cgt.7700454 1:CAS:528:DC%2BD38Xisl2ru7o%3D 11960291 (Pubitemid 34289459)
11. M Cemazar M Golzio G Sersa, et al. 2006 Electrically-assisted nucleic acids delivery to tissues in vivo: where do we stand? Curr Pharm Des 12 3817 3825 10.2174/138161206778559740 1:CAS:528:DC%2BD28XhtFarsb7E 17073680 (Pubitemid 44560715)
12. B Chevallay D Herbage 2000 Collagen-based biomaterials as 3D scaffold for cell cultures: applications for tissue engineering and gene therapy Med Biol Eng Comput 38 211 218 10.1007/BF02344779 1:STN:280:DC%2BD3c3ovVahtw%3D%3D 10829416
13. M Cotten E Wagner 1993 Non-viral approaches to gene therapy Curr Opin Biotechnol 4 705 710 10.1016/0958-1669(93)90053-Y 1:CAS:528:DyaK2cXht1eqsr4%3D 7764468 (Pubitemid 24001486)
14. E Cukierman R Pankov DR Stevens, et al. 2001 Taking cell-matrix adhesions to the third dimension Science 294 1708 1712 10.1126/science.1064829 1:CAS:528:DC%2BD3MXoslCjs7w%3D 11721053 (Pubitemid 33104840)
15. DT Curiel S Agarwal E Wagner, et al. 1991 Adenovirus enhancement of transferring-polylysine-mediated gene delivery Proc Natl Acad Sci USA 88 8850 8854 10.1073/pnas.88.19.8850 1:CAS:528:DyaK38XhtlOn 1681545
16. L Drury 1996 Transformation of bacteria by electroporation Methods Mol Biol 58 249 256 1:CAS:528:DyaK28Xit1Cksbo%3D 8713871
17. V Ganeva B Galutzov J Teissie 1995 Electric field mediated loading of macromolecules in intact yeast cells is critically controlled at the wall level Biochim Biophys Acta 1240 229 236 10.1016/0005-2736(95)00181-6 8541294 (Pubitemid 26004747)
18. J Gehl LM Mir 1999 Determination of optimal parameters for in vivo gene transfer by electroporation, using a rapid in vivo test for cell permeabilization Biochem Biophys Res Commun 261 377 380 10.1006/bbrc.1999.1014 1:CAS:528:DyaK1MXks1Oku70%3D 10425193 (Pubitemid 29392484)
19. S Hacein-Bey-Abina F Le Deist F Carlier, et al. 2002 Sustained correction of X-linked severe combined immunodeficiency by ex vivo gene therapy N Engl J Med 346 1185 1193 10.1056/NEJMoa012616 1:CAS:528:DC%2BD38XivVOmsrg%3D 11961146 (Pubitemid 34984579)
20. DG Harkin ED Hay 1996 Effects of electroporation on the tubulin cytoskeleton and directed migration of corneal fibroblasts cultured within collagen matrices Cell Motil Cytoskeleton 35 345 357 10.1002/(SICI)1097- 0169(1996)35:4<345::AID-CM6>3.0.CO;2-5 1:CAS:528:DyaK2sXisVajsg%3D%3D 8956005 (Pubitemid 26397191)
21. R Heller M Jaroszeski A Atkin, et al. 1996 In vivo gene electroinjection and expression in rat liver FEBS Lett 389 225 228 10.1016/0014-5793(96)00590-X 1:CAS:528:DyaK28XktVOmsb0%3D 8766704 (Pubitemid 26251823)
22. M Hindie M Vayssade M Dufresne, et al. 2006 Interactions of B16F10 melanoma cells aggregated on a cellulose substrate J Cell Biochem 99 96 104 10.1002/jcb.20833 1:CAS:528:DC%2BD28XovVKqsLk%3D 16598743 (Pubitemid 44297953)
23. M Kanduser D Miklavcic M Pavlin 2009 Mechanisms involved in gene electrotransfer using high- and low-voltage pulses-an in vitro study Bioelectrochemistry 74 265 271 10.1016/j.bioelechem.2008.09.002 1:CAS:528:DC%2BD1MXhtVeitLs%3D 18930698
24. A Kikuchi Y Aoki S Sugaya, et al. 1999 Development of novel cationic liposomes for efficient gene transfer into peritoneal disseminated tumor Hum Gene Ther 10 947 955 10.1089/10430349950018346 1:CAS:528:DyaK1MXis12ksbo%3D 10223728 (Pubitemid 29195424)
25. JB Kim R Stein MJ O'Hare 2004 Three-dimensional in vitro tissue culture models of breast cancer-a review Breast Cancer Res Treat 85 281 291 10.1023/B:BREA.0000025418.88785.2b 15111767 (Pubitemid 38987263)
26. GY Lee PA Kenny EH Lee, et al. 2007 Three-dimensional culture models of normal and malignant breast epithelial cells Nat Methods 4 359 365 10.1038/nmeth1015 1:CAS:528:DC%2BD2sXkt1eqsLk%3D 17396127 (Pubitemid 46766981)
27. E Marshall 1999 Gene therapy death prompts review of adenovirus vector Science 286 2244 2245 10.1126/science.286.5448.2244 1:CAS:528:DC%2BD3cXjs1Sq 10636774 (Pubitemid 30016842)
28. D Miklavcic K Beravs D Semrov, et al. 1998 The importance of electric field distribution for effective in vivo electroporation of tissues Biophys J 74 2152 2158 10.1016/S0006-3495(98)77924-X 1:CAS:528:DyaK1cXislWktr0%3D 9591642
29. LM Mir M Belehradek C Domenge, et al. 1991 Electrochemotherapy, a new antitumor treatment: first clinical trial C R Acad Sci III 313 613 618 1:STN:280:DyaK387ksFCltg%3D%3D 1723647
30. LM Mir LF Glass G Sersa, et al. 1998 Effective treatment of cutaneous and subcutaneous malignant tumors by electrochemotherapy Br J Cancer 77 2336 2342 1:CAS:528:DyaK1cXktlCmur0%3D 9649155
31. LM Mir MF Bureau J Gehl, et al. 1999 High-efficiency gene transfer into skeletal muscle mediated by electric pulses Proc Natl Acad Sci USA 96 4262 4267 10.1073/pnas.96.8.4262 1:CAS:528:DyaK1MXjs1ymur8%3D 10200250 (Pubitemid 29190324)
32. KJ Muller VL Sukhorukov U Zimmermann 2001 Reversible electropermeabilization of mammalian cells by high-intensity, ultra-short pulses of submicrosecond duration J Membr Biol 184 161 170 10.1007/s00232-001-0084-3 1:CAS:528:DC%2BD3MXos1Khsrk%3D 11719852 (Pubitemid 33064357)
33. E Neumann K Rosenheck 1972 Permeability changes induced by electric impulses in vesicular membranes J Membr Biol 10 279 290 10.1007/BF01867861 1:STN:280:DyaE2c%2FitlKrsw%3D%3D 4667921
34. E Neumann M Schaefer-Ridder Y Wang, et al. 1982 Gene transfer into mouse lyoma cells by electroporation in high electric fields EMBO J 7 841 845
35. T Nishi K Yoshizato S Yamashiro, et al. 1996 High-efficiency in vivo gene transfer using intraarterial plasmid DNA injection following in vivo electroporation Cancer Res 56 1050 1055 1:CAS:528:DyaK28XhtlKksbg%3D 8640760
36. SY Ong H Dai KW Leong 2006 Inducing hepatic differentiation of human mesenchymal stem cells in pellet culture Biomaterials 27 4087 4097 10.1016/j.biomaterials.2006.03.022 1:CAS:528:DC%2BD28XjslyisbY%3D 16616366
37. Pavlin M, Flisar K, Kandušer M (2010) The role of electrophoresis in gene electrotransfer. J Membr Biol. doi: 10.1007/s00232-010-9276-z
38. H Potter 1988 Electroporation in biology: methods, applications, and instrumentation Anal Biochem 174 361 373 10.1016/0003-2697(88)90035-8 1:CAS:528:DyaL1MXjvVOr 3071177
39. MP Rols J Teissie 1998 Electropermeabilization of mammalian cells to macromolecules: control by pulse duration Biophys J 75 1415 1423 10.1016/S0006-3495(98)74060-3 1:CAS:528:DyaK1cXlvVCru7c%3D 9726943
40. MP Rols C Delteil M Golzio, et al. 1998 In vivo electrically mediated protein and gene transfer in murine melanoma Nat Biotechnol 16 168 171 10.1038/nbt0298-168 1:CAS:528:DyaK1cXpsFKqtg%3D%3D 9487524 (Pubitemid 28078505)
41. S Satkauskas MF Bureau M Puc, et al. 2002 Mechanisms of in vivo DNA electrotransfer: respective contributions of cell electropermeabilization and DNA electrophoresis Mol Ther 5 133 140 10.1006/mthe.2002.0526 1:CAS:528:DC%2BD38XhvFGqsbo%3D 11829520 (Pubitemid 34144829)
42. S Satkauskas F Andre MF Bureau, et al. 2005 Electrophoretic component of electric pulses determines the efficacy of in vivo DNA electrotransfer Hum Gene Ther 16 1194 1201 10.1089/hum.2005.16.1194 1:CAS:528:DC%2BD2MXhtVyrtbfO 16218780 (Pubitemid 41443711)
43. G Sersa 2006 The state-of-the-art of electrochemotherapy before the ESOPE study: advantages and clinical uses Eur J Cancer Suppl 4 52 59 10.1016/j.ejcsup.2006.08.007 (Pubitemid 44666532)
44. G Sersa B Stabuc M Cemazar, et al. 2000 Electrochemotherapy with cisplatin: clinical experience in malignant melanoma patients Clin Cancer Res 6 863 867 1:CAS:528:DC%2BD3cXisVaqtLc%3D 10741708
45. S Simoes V Slepushkin R Gaspar, et al. 1998 Gene delivery by negatively charged ternary complexes of DNA, cationic liposomes and transferrin or fusogenic peptides Gene Ther 5 955 964 10.1038/sj.gt.3300674 1:CAS:528:DyaK1cXksFWrtrk%3D 9813667
46. JR Simon 1993 Transformation of intact yeast cells by electroporation Methods Enzymol 217 478 483 10.1016/0076-6879(93)17085-J 1:CAS:528: DyaK3sXkvVOgsLg%3D 8474347 (Pubitemid 223041660)
47. S Somiari J Glasspool-Malone JJ Drabick, et al. 2000 Theory and in vivo application of electroporative gene delivery Mol Ther 2 178 187 10.1006/mthe.2000.0124 1:CAS:528:DC%2BD38XhvF2ht78%3D 10985947
48. VL Sukhorukov R Reuss D Zimmermann, et al. 2005 Surviving high-intensity field pulses: strategies for improving robustness and performance of electrotransfection and electrofusion J Membr Biol 206 187 201 10.1007/s00232-005-0791-2 1:CAS:528:DC%2BD28XhtFWmsbo%3D 16456714 (Pubitemid 43199137)
49. WB Terzaghi AR Cashmore 1997 Plant cell transfection by electroporation Methods Mol Biol 62 453 462 1:CAS:528:DyaK2sXit1yhsL4%3D 9108540
50. G Tevz D Pavlin U Kamensek, et al. 2008 Gene electrotransfer into murine skeletal muscle: a systematic analysis of parameters for long-term gene expression Technol Cancer Res Treat 7 91 101 1:CAS:528:DC%2BD1cXlslagsrY%3D 18345697
51. A Titomirov S Sukarev E Kistanova 1991 In vivo electroporation and stable transformation of skin cells of newborn mice by plasmid DNA Biochim Biophys Acta 1088 131 134 1:CAS:528:DyaK3MXhtFKhur4%3D 1703441
52. Ušaj M, Trontelj K, Miklavčič D, Kandušer M (2010) Cell-cell electrofusion: optimization of electric field amplitude and hypotonic treatment for mouse melanoma (B16-F1) and Chinese hamster ovary (CHO) cells. J Membr Biol. doi: 10.1007/s00232-010-9272-3
53. J Vienken U Zimmermann 1985 An improved electrofusion technique for production of mouse hybridoma cells FEBS Lett 182 278 280 10.1016/0014-5793(85) 80315-X 1:CAS:528:DyaL2MXitVahsrY%3D 3979550 (Pubitemid 15074352)
54. E Wagner C Plank K Zatloukal, et al. 1992 Influenza virus hemagglutinin HA-2N-terminal fusogenic peptides augment gene transfer by transferring- polylysine-DNA complexes: toward a synthetic virus-like gene-transfer vehicle Proc Natl Acad Sci USA 89 7934 7938 10.1073/pnas.89.17.7934 1:CAS:528: DyaK38Xmt1OgsLY%3D 1518816
55. L Wasungu JM Escoffre A Valette, et al. 2009 A 3D in vitro spheroid model as a way to study the mechanisms of electroporation Int J Pharm 379 278 284 10.1016/j.ijpharm.2009.03.035 1:CAS:528:DC%2BD1MXhtVKjsr3M 19716035
56. H Wolf MP Rols E Boldt, et al. 1994 Control by pulse parameters of electric field-mediated gene transfer in mammalian cells Biophys J 66 524 531 10.1016/S0006-3495(94)80805-7 1:CAS:528:DyaK2cXjtFKqsr4%3D 8161705
57. DA Zaharoff F Yuan 2004 Effects of pulse strength and pulse duration on in vitro DNA electromobility Bioelectrochemistry 62 37 45 10.1016/j.bioelechem. 2003.10.011 1:CAS:528:DC%2BD2cXhsFWgs78%3D 14990324 (Pubitemid 38283503)
58. DA Zaharoff RC Barr CY Li, et al. 2002 Electromobility of plasmid DNA in tumor tissues during electric field-mediated gene delivery Gene Ther 9 1286 1290 10.1038/sj.gt.3301799 1:CAS:528:DC%2BD38XmvVGjsLg%3D 12224011