Quantitative analysis of endocytosis with cytoplasmic pHluorin chimeras

Traffic

Volumn 11, Issue 9, 2010, Pages 1141-1150

  Prosser, Derek C ^a   Whitworth, Karen ^a   Wendland, Beverly ^a  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Cargo; Endocytosis; Multivesicular body; PHluorin; Vacuole

Indexed keywords

GREEN FLUORESCENT PROTEIN; HYDROLASE; PHLUORIN; PROTEINASE; UNCLASSIFIED DRUG;

ACIDIFICATION; ARTICLE; CELL KINETICS; CELL VACUOLE; CHIMERA; CYTOPLASM; ENDOCYTOSIS; ENZYME ACTIVITY; NONHUMAN; PH; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN VARIANT; QUANTITATIVE ANALYSIS; SIGNAL TRANSDUCTION; YEAST;

ENDOCYTOSIS; GREEN FLUORESCENT PROTEINS; HYDROGEN-ION CONCENTRATION; MICROSCOPY, FLUORESCENCE; MODELS, BIOLOGICAL; SACCHAROMYCES CEREVISIAE;

EID: 77955264425     PISSN: 13989219     EISSN: 16000854     Source Type: Journal    
DOI: 10.1111/j.1600-0854.2010.01088.x     Document Type: Article

References (25)

1. Bokman SH, Ward WW. Renaturation of Aequorea green-fluorescent protein. Biochem Biophys Res Commun 1981, 101:1372-1380.
2. Vida TA, Emr SD. A new vital stain for visualizing vacuolar membrane dynamics and endocytosis in yeast. J Cell Biol 1995, 128:779-792.
3. Barysheva OV, Fujii J, Takaesu G, Yoshida S-I. Application of unstable GFP variants to the kinetic study of Legionella pneumophila icm gene expression during infection. Microbiol 2008, 154:1015-1025.
4. Mateus C, Avery SV. Destabilized green fluorescent protein for monitoring dynamic changes in yeast gene expression with flow cytometry. Yeast 2000, 16:1313-1323.
5. Andersen JB, Sternberg C, Poulsen LK, Bjorn SP, Givskov M, Molin S. New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Appl Environ Microbiol 1998, 64:2240-2246.
6. Miesenbock G, De Angelis DA, Rothman JE. Visualizing secretion and synaptic transmission with pH-sensitive green fluorescent proteins. Nature 1998, 394:192-195.
7. Sankaranarayanan S, De Angelis D, Rothman JE, Ryan TA. The use of pHluorins for optical measurements of presynaptic activity. Biophys J 2000, 79:2199-2208.
8. Pena A, Ramirez J, Rosas G, Calahorra M. Proton pumping and the internal pH of yeast cells, measured with pyranine introduced by electroporation. J Bacteriol 1995, 177:1017-1022.
9. Plant PJ, Manolson MF, Grinstein S, Demaurex N. Alternative mechanisms of vacuolar acidification in H+-ATPase-deficient yeast. J Biol Chem 1999, 274:37270-37279.
10. Brett CL, Tukaye DN, Mukherjee S, Rao R. The yeast endosomal Na+(K+)/H+ exchanger Nhx1 regulates cellular pH to control vesicle trafficking. Mol Biol Cell 2005, 16:1396-1405.
11. Merrifield CJ, Perrais D, Zenisek D. Coupling between clathrin-coated-pit invagination, cortactin recruitment, and membrane scission observed in live cells. Cell 2005, 121:593-606.
12. Gottfried I, Ehrlich M, Ashery U. HIP1 exhibits an early recruitment and a late stage function in the maturation of coated pits. Cell Mol Life Sci 2009, 66:2897-2911.
13. Eiden-Plach A, Zagorc T, Heintel T, Carius Y, Breinig F, Schmitt MJ. Viral preprotoxin signal sequence allows efficient secretion of green fluorescent protein by Candida glabrata, Pichia pastoris, Saccharomyces cerevisiae, and Schizosaccharomyces pombe. Appl Environ Microbiol 2004, 70:961-966.
14. Li J, Xu H, Bentley WE, Rao G. Impediments to secretion of green fluorescent protein and its fusion from Saccharomyces cerevisiae. Biotechnol Prog 2002, 18:831-838.
15. Hurley JH, Emr SD. The ESCRT complexes: structure and mechanism of a membrane-trafficking network. Annu Rev Biophys Biomol Struct 2006, 35:277-298.
16. Longtine MS, McKenzie A, Demarini DJ, Shah NG, Wach A, Brachat A, Philippsen P, Pringle JR. Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae. Yeast 1998, 14:953-961.
17. Urbanowski JL, Piper RC. Ubiquitin sorts proteins into the intralumenal degradative compartment of the late-endosome/vacuole. Traffic 2001, 2:622-630.
18. Maldonado-Baez L, Dores MR, Perkins EM, Drivas TG, Hicke L, Wendland B. Interaction between Epsin/Yap180 adaptors and the scaffolds Ede1/Pan1 is required for endocytosis. Mol Biol Cell 2008, 19:2936-2948.
19. Aguilar RC, Longhi SA, Shaw JD, Yeh L-Y, Kim S, Schön A, Freire E, Hsu A, McCormick WK, Watson HA, Wendland B. Epsin N-terminal homology domains perform an essential function regulating Cdc42 through binding Cdc42 GTPase-activating proteins. Proc Natl Acad Sci U S A 2006, 103:4116-4121.
20. Overstreet E, Chen X, Wendland B, Fischer JA. Either part of a Drosophila epsin protein, divided after the ENTH domain, functions in endocytosis of Delta in the developing eye. Curr Biol 2003, 13:854-860.
21. Babst M, Sato TK, Banta LM, Emr SD. Endosomal transport function in yeast requires a novel AAA-type ATPase, Vps4p. EMBO J 1997, 16:1820-1831.
22. Rue SM, Mattei S, Saksena S, Emr SD. Novel Ist1-Did2 complex functions at a late step in multivesicular body sorting. Mol Biol Cell 2008, 19:475-484.
23. Dulic V, Egerton M, Elguindi I, Raths S, Singer B, Riezman H. Yeast endocytosis assays. Methods Enzymol 194:697-710.
24. Tran JH, Chen C-J, Emr S, Schekman R. Cargo sorting into multivesicular bodies in vitro. Proc Natl Acad Sci U S A 2009, 106:17395-17400.
25. Dimaano C, Jones CB, Hanono A, Curtiss M, Babst M. Ist1 regulates Vps4 localization and assembly. Mol Biol Cell 2008, 19:465-474.