Perfusion culture system: Synovial fibroblasts modulate articular chondrocyte matrix synthesis in vitro

Tissue and Cell

Volumn 42, Issue 3, 2010, Pages 151-157

  Steinhagen, J ^a   Bruns, J ^a   Niggemeyer, O ^b   Fuerst, M ^b   Ruther W ^a   Schunke M ^c   Kurz, B ^c  

^a UNIVERSITY MEDICAL CENTER HAMBURG EPPENDORF   (Germany)

^b Klinikum Bad Bramstedt   (Germany)

^c UNIVERSITY OF KIEL   (Germany)

Author keywords

Chondrocytes; Co culture; Perfusion chamber; Synovial fibroblasts

Indexed keywords

COLLAGEN TYPE 1; COLLAGEN TYPE 2; DNA; HYDROGEN PEROXIDE; LACTATE DEHYDROGENASE; METHYLENE BLUE; PROLINE; PROTEOGLYCAN; REACTIVE OXYGEN METABOLITE; SULFATE;

ANIMAL CELL; ARTICLE; CARTILAGE CELL; CATTLE; CELL ASSAY; CELL CULTURE; CELL INTERACTION; CELL LABELING; CELL METABOLISM; CELL PROLIFERATION; CELL TYPE; COCULTURE; COLLAGEN SYNTHESIS; CONTROLLED STUDY; DNA CONTENT; ENZYME LINKED IMMUNOSORBENT ASSAY; ENZYME RELEASE; EXTRACELLULAR MATRIX; FIBROBLAST; IMMUNOHISTOCHEMISTRY; IN VITRO STUDY; KNEE; MEMBRANE DAMAGE; MONOCULTURE; NONHUMAN; PROTEIN SYNTHESIS; PROTEOGLYCAN SYNTHESIS; SUPERNATANT; SYNOVIOCYTE; SYNOVIUM; ANIMAL; ARTICULAR CARTILAGE; BOVINAE; CELL SHAPE; CULTURE TECHNIQUE; CYTOLOGY; ENZYMOLOGY; EXTRACELLULAR SPACE; METABOLISM; PERFUSION; PROCEDURES; SECRETION (PROCESS); SYNOVIAL FLUID;

ANIMALS; CARTILAGE, ARTICULAR; CATTLE; CELL CULTURE TECHNIQUES; CELL PROLIFERATION; CELL SHAPE; CHONDROCYTES; COCULTURE TECHNIQUES; COLLAGEN TYPE II; EXTRACELLULAR MATRIX; EXTRACELLULAR SPACE; FIBROBLASTS; IMMUNOHISTOCHEMISTRY; L-LACTATE DEHYDROGENASE; PERFUSION; PROTEIN BIOSYNTHESIS; PROTEOGLYCANS; SYNOVIAL FLUID;

EID: 77955262400     PISSN: 00408166     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.tice.2010.03.003     Document Type: Article

References (27)

1. Blanco F.J., Lotz M. IL-1-induced nitric oxide inhibits chondrocyte proliferation via PGE2. Exp. Cell Res. 1995, 218(1):319-325.
2. Chen V., Croft D., Purkis P., et al. Co-culture of synovial fibroblasts and differentiated U937 cells is sufficient for high interleukin-6 but not interleukin-1beta or tumour necrosis factor-alpha release. Br. J. Rheumatol. 1998, 37(2):148-156.
3. D'Andrea P., Calabrese A., Grandolfo M. Intercellular calcium signalling between chondrocytes and synovial cells in co-culture. Biochem. J. 1998, 329(Pt 3):681-687.
4. Engle J.M., Georgescu H.I., Evans C.H. Interleukin-1 alpha as an autocrine regulator in rabbit synovial fibroblasts. Inflamm. Res. 1997, 46(Suppl. 2):S175-S176.
5. Evans CH, Georgescu HI, Mendelow D, et al., 1987. Modulation of chondrocyte metabolism by cytokines produced by a synovial cell line. Development and diseases of cartilage and bone matrix. Alan R. Liss, Inc., pp. 319-329.
6. Huch K., Stove J., Gunther K.P., et al. Interactions between human osteoarthritic chondrocytes and synovial fibroblasts in co-culture. Clin. Exp. Rheumatol. 2001, 19(1):27-33.
7. Israel H.W., Salpeter M.M., Steward F.C. The incorporation of radioactive proline into cultured cells. Interpretations based on radioautography and electronmicroscopy. J. Cell Biol. 1968, 39(3):698-715.
8. Iwamoto M., Koike T., Nakashima K., et al. Interleukin 1: a regulator of chondrocyte proliferation. Immunol. Lett. 1989, 21(2):153-156.
9. Jikko A., Wakisaka T., Iwamoto M., et al. Effects of interleukin-6 on proliferation and proteoglycan metabolism in articular chondrocyte cultures. Cell Biol. Int. 1998, 22(9-10):615-621.
10. Katrib A., McNeil H.P., Youssef P.P. What can we learn from the synovium in early rheumatoid arthritis?. Inflamm. Res. 2002, 51(4):170-175.
11. Kurz B., Steinhagen J., Schuenke M. Articular chondrocytes and synoviocytes in a co-culture system: influence on reactive oxygen species-induced cytotoxicity and lipid peroxidation. Cell Tissue Res. 1999, 296(3):555-563.
12. Lee D.A., Salih V., Stockton E.F., et al. Effect of normal synovial fluid on the metabolism of articular chondrocytes in vitro. Clin. Orthop. 1997, (342):228-238.
13. Legendre P., Richards C.D., Rafferty J.A., et al. The expression of matrix metalloproteinases and their inhibitors by pig synovial cells and their regulation by combinations of cytokines and growth factors. Comp. Biochem. Physiol. B 1993, 106(3):691-704.
14. Levick J.R., McDonald J.N. Fluid movement across synovium in healthy joints: role of synovial fluid macromolecules. Ann. Rheum Dis. 1995, 54(5):417-423.
15. Lin C.W., Robbins P.D., Georgescu H.I., et al. Effects of immortalization upon the induction of matrix metalloproteinases in rabbit synovial fibroblasts. Exp. Cell Res. 1996, 223(1):117-126.
16. Livne E. In vitro response of articular cartilage from mature mice to human transforming growth factor beta. Acta Anat. 1994, 149(3):185-194.
17. Malaviya P., Nerem R.M. Fluid-induced shear stress stimulates chondrocyte proliferation partially mediated via TGF-beta1. Tissue Eng. 2002, 8(4):581-590.
18. Minuth W.W., Schumacher K., Strehl R., et al. Physiological and cell biological aspects of perfusion culture technique employed to generate differentiated tissues for long term biomaterial testing and tissue engineering. J. Biomater. Sci. Polym. Ed. 2000, 11(5):495-522.
19. Nevo Z., Silver J., Chorev Y., et al. Adhesion characteristics of chondrocytes cultured separately and in co-cultures with synovial fibroblasts. Cell Biol. Int. 1993, 17(3):255-273.
20. Norby D.P., Malemud C.J., Sokoloff L. Differences in the collagen types synthesized by lapine articular chondrocytes in spinner and monolayer culture. Arthritis Rheum 1977, 20(2):709-716.
21. Pratta M.A., Scherle P.A., Yang G., et al. Induction of aggrecanase 1 (ADAM-TS4) by interleukin-1 occurs through activation of constitutively produced protein. Arthritis Rheum 2003, 48(1):119-133.
22. Ritchlin C. Fibroblast biology. Effector signals released by the synovial fibroblast in arthritis. Arthritis Res. 2000, 2(5):356-360.
23. Sandy J.D., Gamett D., Thompson V., et al. Chondrocyte-mediated catabolism of aggrecan: aggrecanase-dependent cleavage induced by interleukin-1 or retinoic acid can be inhibited by glucosamine. Biochem. J. 1998, 335(Pt. 1):59-66.
24. Shingu M., Isayama T., Yasutake C., Naono T., et al. Role of oxygen radicals and IL-6 in IL-1-dependent cartilage matrix degradation. Inflammation 1994, 18(6):613-623.
25. Shinmei M., Masuda K., Kikuchi T., et al. The role of cytokines in chondrocyte mediated cartilage degradation. J. Rheumatol. 1989, (Suppl. 18):32-34.
26. Stockwell R.A. Metabolism of Cartilage. Cartilage (1). Structure, Function and Biochemistry 1983, 254-256. Academic Press. B.K. Hall (Ed.).
27. von der Mark K., Gauss V., von der Mark H., et al. Relationship between cell shape and type of collagen synthesised as chondrocytes lose their cartilage phenotype in culture. Nature 1977, 267(5611):531-532.