Prolonged ingestion of prehydrolyzed whey protein induces little or no change in digestive enzymes, but decreases glutaminase activity in exercising rats

Journal of Medicinal Food

Volumn 13, Issue 4, 2010, Pages 992-998

  Nery Diez, Ana Cláudia C ^a   Carvalho, Iara R ^a   Amaya Farfán, Jaime ^a   Abecia Soria, Maria Inés ^a   Miyasaka, Célio K ^a   Ferreira, Clécio Da S ^a  

^a UNIVERSITY OF CAMPINAS   (Brazil)

Author keywords

antioxidants; antistress; bioactive peptides; dietary protein; intestinal proteases; oxidative stress; peptide absorption; physical activity; protein digestion; proteolysis

Indexed keywords

CHYMOTRYPSIN; CYTOSOL AMINOPEPTIDASE; GLUTAMINASE; MILK PROTEIN; PEPSIN A; PROTEIN HYDROLYSATE; TRYPSIN;

ANIMAL EXPERIMENT; ARTICLE; DIGESTIVE SYSTEM; ENZYME ACTIVITY; EXERCISE; EXHAUSTION; HYDROLYSIS; INGESTION; MALE; NONHUMAN; PRIORITY JOURNAL; RAT; TRAINING; TREADMILL; WHEY;

ANIMALS; DIETARY PROTEINS; DIGESTIVE SYSTEM; EXERCISE; GLUTAMINASE; HUMANS; MALE; MILK PROTEINS; MODELS, ANIMAL; PROTEIN HYDROLYSATES; RATS; RATS, WISTAR;

ANIMALIA; MAMMALIA; RATTUS; RATTUS NORVEGICUS;

EID: 77955211345     PISSN: 1096620X     EISSN: None     Source Type: Journal    
DOI: 10.1089/jmf.2009.0149     Document Type: Article

References (40)

1. Boza J, Jiménez J, Baró L, et al.: Effects of native and hy-drolyzed whey protein on intestinal repair of severely starved rats at weaning. J Pediatr Gastroenterol Nutr 1996;22:186-193.
2. Ziegler EE, Jeter JM, Drulis JM, et al.: Formula with reduced content of improved, partially hydrolyzed protein and probiotics: infant growth and health. Monatsschr Kinderheilkd 2003;151:65-71.
3. Moreno YF, Sgarbieri VC, da Silva MN, et al.: Features of whey protein concentrate supplementation in children with rapidly progressive HIV infection. J Trop Pediatr 2006;52:34-38.
4. von Berg A, Koletzko S, Filipiak-Pittroff B, et al.: Certain hy-drolyzed formulas reduce the incidence of atopic dermatitis but not that of asthma: three-year results of the German Infant Nutritional Intervention Study. J Allergy Clin Immunol 2007;119: 718-725.
5. Tassi EM, Amaya-Farfán J, Azevedo R: Hydrolyzed a-lactalbumin as a source of protein to the exercising. Nutr Res 1998;18:875-881.
6. Pimenta FMV, Abecia-Soria MI, Auler F, et al.: Physical performance of exercise young rats fed hydrolyzed whey protein at a sub-optimal level. Int Dairy J 2006;16:984-991.
7. Hori G, Wang MF, Chan YC, et al.: Soy protein hydrolyzate with bound phospholipids reduces serum cholesterol levels in hyper-cholesterolemic adult male volunteers. Biosci Biotechnol Bio-chem 2001;65:72-78.
8. Colombo C, Biffi A, Agosti M, et al.: Pancreatic development in newborn guinea pigs fed intact or low-hydrolyzed protein formulas. J Pediatr Gastroenterol Nutr 2005;41:644-649.
9. Pins JJ, Keenan JM: Effects of whey peptides on cardiovascular disease risk factors. J Clin Hypertens 2006;8:775-782.
10. Hayes A, Cribb PJ: Effect of whey protein isolate on strength, body composition and muscle hypertrophy during resistance training. Curr Opin Clin Nutr Metab Care 2008;11:40-44.
11. Colbert LB, Decker EA: Antioxidant activity of an ultrafiltration permeate from acid whey. J Food Sci 1991;56:1248-1250.
12. Peña-Ramos EA, Xiong YL: Antioxidative activity of whey protein hydrolysates in a liposomal system. J Dairy Sci 2001;84:2577-2583.
13. Cheison SC, Wang Z, Xu S-Y: Preparation of whey protein hy-drolysates using a single-and two-stage enzymatic membrane reactor and their immunological and antioxidant properties: characterization by multivariate data analysis. J Agric Food Chem 2007;55:3896-3904.
14. Pilhanto A: Antioxidative peptides derived from milk proteins. Int Dairy J 2006;16:1306-1314.
15. Peng X, Xiong YL, Kong B: Antioxidant activity of peptide fractions from whey protein hydrolyzates as measured by electron spin resonance. Food Chem 2009;113:196-201.
16. Pacheco MTB, Sgarbieri VC: Effect of different hydrolysates of whey protein on hepatic glutathione content in mice. J Med Food 2005;8:337-342.
17. Reeves PG, Nielsen FH, Fahey GC Jr: AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition Ad Hoc Writing Committee on the reformulation of the AIN-76A rodent diet. J Nutr 1993;123:1939-1951.
18. Adler-Nissen J: Determination of the degree of hydrolysis of food protein hydrolysates by trinitrobenzenesulfonic acid. J Agric Food Chem 1979;27:1256-1262.
19. Official Methods of Analysis of AOAC International, 18th ed. AOAC International, Gaithersburg, MD, 2006.
20. Smolka MB, Zoppi CC, Alves AA, et al.: HSP72 as a complementary protection against oxidative stress induced by exercise in the soleus muscle of rats. Am J Physiol Regul Integr Comp Physiol 2000;279:1539-1545.
21. Anson ML: The estimation of pepsin, trypsin, papain, and ca-thepsin with hemoglobin. J Gen Physiol 1938;22:79-89.
22. Curthoys NP, Lowry OH: The distribution of glutaminase iso-enzymes in the various structure of the nephron in normal, aci-dotic and alkalotic rat kidney. J Biol Chem 1973;248:162-168.
23. Bergmeyer HU: Glutaminase. In: Methods of Enzymatic Analysis (Bergmeyer, HU ed.). Academic Press, New York, 1971, pp.1704-1708.
24. Ohtani S, Shirasu K, Ogawara K, et al.: Evaluation of inhibitory activity of casein on proteases in rat intestine. Pharm Res 2003; 20:611-617.
25. Rick W: Chymotrypsin. In: Methods of Enzymatic Analysis, 2nd ed. (Bergmeyer HU, ed.). Academic Press, New York, 1974, pp. 1006-1012.
26. Rick W: Trypsin. In: Methods of Enzymatic Analysis, 2nd ed. (Bergmeyer HU, ed.). Academic Press, New York, 1974, pp. 1013-1024.
27. Appel W: Peptidases. In: Methods of Enzymatic Analysis, 2nd ed. (Bergmeyer HU, ed.). Academic Press, New York, 1974, pp. 954-958.
28. White JA, Hart RJ, Fry JC: An evaluation of the Waters Pico-Tag system for the amino acid analysis of food materials. J Autom Chem 1986;8:170-177.
29. Hangen SR, Frost B, Augustin J: Precolumn phenylisothiocya-nate derivatization and liquid chromatography of amino acids in food. J Assoc Off Anal Chem 1989;72:912-916.
30. Subcommittee on Laboratory Animal Nutrition, Committee on Animal Nutrition, Board on Agriculture, National Research Council: Nutrient Requirements of Laboratory Animals, 4th revised ed. National Academies Press, Washington, DC, 1995.
31. Ramos AG: Utilization of whey proteins by the rat [M.S. Thesis]. School of Food Engineering, University of Campinas, Campinas, Brazil, 2001, p. 112.
32. Coppi G, Bonardi G, Gaetani MG: Experimental gastric ulcers and uropepsinogen excretion in rat. J Pharm Pharmacol 1971;23:722-723.
33. Lindberg T, Ohlsson K, Weström B: Protease inhibitors and their relation to protease activity in human milk. Pediatr Res 1982;16:479-483.
34. Pinkus LM, Windmueller HG: Phosphate-dependent glutaminase of small intestine and localization and role in intestinal gluta-mine. Arch Biochem Biophys 1977;182:506-517.
35. Masola B, Zvinavashe E: Phosphate-dependent glutaminase in enterocyte mitochondria and its regulation by ammonium and other ions. Amino Acids 2003;24:427-434.
36. França SCA, Neto TLB, Agresta MC, et al.: Resposta divergente da testosterona e do cortisol séricos em atletas masculinos após uma corrida de maratona. Arq Bras Endocrinol Metab 2003;50:1082-1087.
37. Acevedo EO, Kraemer RR, Kamimori GH, et al.: Stress hormones, effort sense, and perceptions of stress during incremental exercise: an exploratory investigation. J Strength Cond Res 2007;21:283-288.
38. Minetto MA, Lanfranco F, Baldi M, et al.: Corticotroph axis sensitivity after exercise: comparison between elite athletes and sedentary subjects. J Endocrinol Invest 2007;30:215-223.
39. Salleh M, Ardawi M, Majzoub MF, et al.: Effect of glucocorti-coid treatment on glucose and glutamine metabolism by the small intestine of the rat. Clin Sci 1988;75:93-100.
40. Peña-Ramos EA, Xiong YL, Arteaga GE: Fractionation and characterization for antioxidant activity of hydrolyzed whey protein. J Sci Food Agric 2004;84:1908-1918.