PCR-DGGE analysis of bacterial population attached to the bovine rumen wall

Folia Microbiologica

Volumn 55, Issue 4, 2010, Pages 345-348

  Lukas F ^a   Simunek J ^a   Mrazek J ^a   Kopecny J ^a  

^a INSTITUTE OF ANIMAL PHYSIOLOGY AND GENETICS   (Czech Republic)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; RIBOSOME DNA; RNA 16S;

ANIMAL; ARTICLE; BACTERIUM; BIODIVERSITY; CATTLE; CLASSIFICATION; DNA DENATURATION; FEMALE; GENETICS; ISOLATION AND PURIFICATION; MALE; MICROBIOLOGY; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; RUMINANT STOMACH; BACTERIA; BOVINAE;

ANIMALS; BACTERIA; BIODIVERSITY; CATTLE; DNA, BACTERIAL; DNA, RIBOSOMAL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FEMALE; MALE; NUCLEIC ACID DENATURATION; POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S; RUMEN;

ANIMALIA; BACTERIA (MICROORGANISMS); BOVINAE;

EID: 77955162722     PISSN: 00155632     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12223-010-0055-0     Document Type: Article

References (15)

1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J.: Basic local alignment search tool. J. Mol. Biol. 215, 403-410 (1990).
2. Cheng K. J., McAllister T. A.: The rumen microbial ecosystem, pp. 492-522 in C. S Stewart (Ed.): Compartimentation in the Rumen. Blackie Academic and Professional, London 1997.
3. Cheng K. J., McCowan R. P., Costerton J. W.: Adherent epithelial bacteria in ruminants and their roles in digestive tract function. Am. J. Clin. Nutr. 32, 139-148 (1979).
4. Cho S. J., Cho K. M., Shin E. C., Lim W. J., Hong S. Y., Choi B. R., Kang J. M., Lee S. M., Kim Y. H., Kim H., Yun H. D.: 16S rDNA analysis of bacterial diversity in three fractions of cow rumen. J. Microbiol. Biotechnol. 16, 92-101 (2006).
5. Larue R., Yu Z. T., Parisi V. A., Egan A. R., Morrison M.: Novel microbial diversity adherent to plant biomass in the herbivore gastrointestinal tract, as revealed by ribosomal intergenic spacer analysis and rrs gene sequencing. Environ. Microbiol. 7, 530-543 (2005).
6. McAllister T. A., Bae H. D., Jones G. A., Cheng K. J.: Microbial attachment and feed digestion in the rumen. J. Anim. Sci. 72, 3004-3018 (1994).
7. McCowan R. P., Cheng K. J., Bailey C. B. M., Costerton J. W.: Adhesion of bacteria to epithelial cells within the reticulorumen of cattle. Appl. Environ. Microbiol. 35, 149-155 (1978).
8. McCowan R. P., Cheng K. J., Costerton J. W.: Adherent bacterial populations on the bovine rumen wall: distribution pattern of adherent bacteria. Appl. Environ. Microbiol. 39, 233-241 (1980).
9. Mead L. J., Jones G. A.: Isolation and presumptive identification of adherent epithelial bacteria from the ovine rumen wall. Appl. Environ. Microbiol. 41, 1020-1028 (1979).
10. Mitsumori M., Ajisaka N., Tajima K., Kajikawa H., Kurihara M.: Detection of Proteobacteria from the rumen by PCR using methanotroph-specific primers. Lett. Appl. Microbiol. 35, 251-255 (2002).
11. Muyzer G., De Wall E. C., Uitterlinden A. G.: Profiling of complex microbial population by denaturing gradient gel electrophoresis analysis of polymerase chain reaction - amplified genes coding for 16S rRNA. Appl. Environ. Microbiol. 59, 695-700 (1993).
12. Sadet S., Martin C., Meunier B., Morgavi D. P.: PCR-DGGE analysis reveals a distinct diversity in the bacterial population attached to the rumen epithelium. Animal1, 939-944 (2007).
13. Temmerman R., Scherlinck I., Huys G., Swings J.: Culture independent analysis of probiotic products by denaturing gradient gel electrophoresis. Appl. Environ. Microbiol. 69, 220-226 (2003).
14. Vandamme P., Pot B., Gills M., De Vos P., Kersters K., Swings J.: Polyphasic taxonomy, a consensus approach to bacterial systematic. Microbiol. Rev. 60, 407-438 (1996).
15. Wallace R. J., Cheng K. J., Dinsdale D., Orskov E. R.: An independent microbial flora of the epithelium and its role in the ecomicrobiology of the rumen. Nature279, 424-426 (1979).