Clinical study on the effects of a cosmetic product on dermal extracellular matrix components using a high-resolution multiphoton tomograph

Skin Research and Technology

Volumn 16, Issue 3, 2010, Pages 305-310

  Bazin, R ^a   Flament, F ^a   Colonna, A ^a   Le Harzic, R ^b   Buckle R ^b   Piot, B ^a   Laize F ^a   Kaatz, M ^c   Konig K ^b   Fluhr, J W ^c,d  

^a L ORÉAL RECHERCHE   (France)

^b FRAUNHOFER INSTITUTE FOR BIOMEDICAL ENGINEERING   (Germany)

^c FRIEDRICH SCHILLER UNIVERSITY JENA   (Germany)

^d Bioskin   (Germany)

Author keywords

Dermal structures; Extracellular matrix; Multiphoton Spectroscopy; Multiphoton tomography; Second harmonic generation

Indexed keywords

COLLAGEN; EMULSIFICATION; ENZYME INHIBITION; HARMONIC ANALYSIS; MULTIPHOTON PROCESSES; NONLINEAR OPTICS; TOMOGRAPHY;

DERMAL STRUCTURE; EXTRACELLULAR MATRICES; MULTIPHOTON SPECTROSCOPY; MULTIPHOTON TOMOGRAPHY; MULTIPHOTONS; SECOND HARMONIC GENERATION;

HARMONIC GENERATION;

COSMETIC; EMOLLIENT AGENT; PLANT EXTRACT;

ADULT; AGED; ARTICLE; AUTOFLUORESCENCE IMAGING; CONTROLLED STUDY; CREAM; DERMIS; EXTRACELLULAR MATRIX; FEMALE; FOREARM; HUMAN; HUMAN EXPERIMENT; IMAGE ANALYSIS; IMAGE QUALITY; JASMINE; LIGHT RELATED PHENOMENA; MULTIPHOTON TOMOGRAPHY; NORMAL HUMAN; SECOND HARMONIC GENERATION; SKIN SURFACE; SOYBEAN; TOMOGRAPHY; CYTOLOGY; DEVICES; DRUG EFFECTS; EPILUMINESCENCE MICROSCOPY; ESTHETIC SURGERY; MIDDLE AGED; MULTIPHOTON MICROSCOPY; PROCEDURES; RANDOMIZED CONTROLLED TRIAL; THEORETICAL MODEL;

AGED; COSMETIC TECHNIQUES; COSMETICS; DERMIS; DERMOSCOPY; EMOLLIENTS; EXTRACELLULAR MATRIX; FEMALE; HUMANS; JASMINUM; MICROSCOPY, FLUORESCENCE, MULTIPHOTON; MIDDLE AGED; MODELS, THEORETICAL; PLANT EXTRACTS; SOYBEANS;

EID: 77955128406     PISSN: 0909752X     EISSN: 16000846     Source Type: Journal    
DOI: 10.1111/j.1600-0846.2010.00432.x     Document Type: Article

References (16)

1. Denk W, Strickler JH, Webb WW. Two-photon laser scanning microscopy. Science 1990, 248:73-76.
2. Hendriks RFM, Lucassen GW. Two photon fluorescence microscopy of in vivo human skin. Progress in Biomedical Optics and Imaging 2000, 1:116-121.
3. König K. Multiphoton microscopy in life sciences. J Microsc 2000, 200((Part 2)):83-104.
4. König K, Peuckert C, Riemann I, Wollina U. Non-invasive 3D optical biopsy of human skin with NIR-femtosecond laser pulses for diagnosis of dermatological disorders. Proc SPIE 2002, 4620:190-201.
5. Masters BR. Three-dimensional confocal microscopy of human skin in vivo: autofluorescence of human skin. Bioimages 1996, 4:13-19.
6. Masters BR, So PTC, Gratton E. Multiphoton excitation fluorescence microscopy andspectroscopy of in vivo human skin. Biophys J 1997, 72:2405-2412.
7. Masters BR, So PTC, Gratton E. Multiphoton excitation microscopy of in vivo human skin. Ann NY Acad Sci 1998, 838:58-67.
8. Rajadhyaksha M, Rox Anderson R, Webb RH. Video-rate confocal scanning laser microscope for imaging human tissues in vivo. Appl Optics 1999, 38:2105-2115.
9. Rajadhyaksha M, Gonzalez S, Zavislan JM, Anderson RR, Webb RH. In vivo confocal laser scanning laser microscopy of human skin II: advances in instrumentation and comparison with histology. J Invest Dermatol 1999, 113:293-303.
10. König K, Riemann I. High-resolution multiphoton tomography of human skin with subcellular spatial resolution and picosecond time resolution. J Biomed Opt 2003, 8:432-439.
11. König K, Ehlers A, Stracke F, Riemann I. In vivo drug screening in human skin using femtosecond laser multiphoton tomography. Skin Pharmacol Physiol 2006, 19:78-88.
12. Krutmann J. Skin aging. Hautarzt 2003, 54:833-838.
13. Schürer NY. Anti-aging. Facts and visions. Hautarzt 2003, 54:833-838.
14. Koehler MJ, König K, Elsner P, Bückle R, Kaatz M. In vivo assessment of human skin aging by multiphoton laser scanning tomography. Opt Lett 2006, 31:2879-2881.
15. Lin SJ, Wu R, Tan HY, Lo W, Lin WC, Young TH, Hsu CJ, Chen JS, Jee SH, Dong C. Evaluating cutaneous photoaging by use of multiphoton fluorescence and second harmonic generation microscopy. Opt Lett 2005, 30:2275-2277.
16. Koehler MJ, Hahn S, Preller, Elsner P, Ziemer M, Bauer A, König K, Bückle R, Fluhr JW, Kaatz M. Morphological skin ageing criteria by multiphoton laser scanning tomography: non-invasive in vivo scoring of the dermal fibre network. Exp Derm 2008, 17:519-523.