A dynamic ratio of the α+ and α- isoforms of the tight junction protein ZO-1 is characteristic of Caco-2 cells and correlates with their degree of differentiation

Cell Biology International

Volumn 34, Issue 6, 2010, Pages 669-678

  Ciana, Annarita ^a   Meier, Katharina ^b   Daum, Nicole ^b   Gerbes, Stefan ^c   Veith, Michael ^c   Lehr, Claus Michael ^b   Minetti, Giampaolo ^a  

^a UNIVERSITY OF PAVIA   (Italy)

^b SAARLAND UNIVERSITY   (Germany)

^c INM LEIBNIZ INSTITUTE FOR NEW MATERIALS   (Germany)

Author keywords

Caco 2; Endothelial cell line; Epithelial cell line; Pancreatic stem cell; Zonula occludens 1 (ZO 1) isoform

Indexed keywords

ISOPROTEIN; MICROSOMAL AMINOPEPTIDASE; PROTEIN ZO1; MEMBRANE PROTEIN; MESSENGER RNA; PHOSPHOPROTEIN; ZONULA OCCLUDENS-1 PROTEIN;

ARTICLE; CELL DIFFERENTIATION; CELL GROWTH; CELL STRAIN CACO 2; CONTROLLED STUDY; CORRELATION FUNCTION; ENZYME SUBSTRATE; EPITHELIUM CELL; HUMAN; HUMAN CELL; IN VIVO CULTURE; INTESTINE CELL; MOLECULAR DYNAMICS; NUCLEOTIDE SEQUENCE; PANCREAS CELL; PROTEIN EXPRESSION; PROTEIN STABILITY; TUMOR CELL LINE; ALTERNATIVE RNA SPLICING; CELL LINE; GENETICS; METABOLISM;

ALTERNATIVE SPLICING; ANTIGENS, CD13; CACO-2 CELLS; CELL DIFFERENTIATION; CELL LINE; HUMANS; MEMBRANE PROTEINS; PHOSPHOPROTEINS; PROTEIN ISOFORMS; RNA, MESSENGER;

EID: 77955067882     PISSN: 10656995     EISSN: 10958355     Source Type: Journal    
DOI: 10.1042/CBI20090067     Document Type: Article

References (38)

1. Aksamitiene E, Hoek JB, Kholodenko B, Kiyatkin A. Multistrip Western blotting to increase quantitative data output. Electrophoresis 2007;28:3163-73.
2. Anderson JM, Fanning AS, Lapierre L, Van Itallie CM. Zonula occludens (ZO)-1 and ZO-2: membrane-associated guanylate kinase homologues (MAGuKs) of the tight junction. Biochem Soc Trans 1995;23:470-5.
3. Artursson P, Palm K, Luthman K. Caco-2 monolayers in experimental and theoretical predictions of drug transport. Adv Drug Deliv Rev 2001;46:27-43.
4. Balda MS, Anderson JM. Two classes of tight junctions are revealed by ZO-1 isoforms. Am J Physiol 1993;264:C918-24.
5. Balda MS, Garrett MD, Matter K. The ZO-1-associated Y-box factor ZONAB regulates epithelial cell proliferation and cell density. J Cell Biol 2003;60:423-32.
6. Brown J, Reading SJ, Jones S, Fitchett CJ, Howl J, Martin A et al. Critical evaluation of ECV304 as a human endothelial cell model defined by genetic analysis and functional responses: a comparison with the human bladder cancer derived epithelial cell line T24/83. Lab Invest 2000;80:37-45.
7. Chen W, Tang F, Horie K, Borchardt RT. Caco-2 cell monolayers as a model for studies of drug transport across human intestinal epithelium. In: Lehr CM, editor. Cell culture models of biological barriers, in-vitro test systems for drug absorption and delivery. London: Taylor and Francis; 2002. P. 143-63.
8. Cho KO, Hunt CA, Kennedy MB. The rat brain postsynaptic density fraction contains a homolog of the Drosophila discs-large tumor suppressor protein. Neuron 1992;9:929-42.
9. Duesberg P, Li R, Fabarius A, Hehlmann R.The chromosomal basis of cancer. Cell Oncol 2005;27:293-318.
10. Eckert JJ, Fleming TP. Tight junction biogenesis during early development. Biochim Biophys Acta 2008;1778:717-28.
11. Engle MJ, Goetz GS, Alpers DH. Caco-2 cells express a combination of colonocyte and enterocyte phenotypes. J Cell Physiol 1998;174:362-9.
12. Fanning AS, Jameson BJ, Jesaitis LA, Anderson JM. The tight junction protein ZO-1 establishes a link between the transmembrane protein occludin and the actin cytoskeleton. J Biol Chem 1998;273:29745-53.
13. Fanning AS, Ma TY, Anderson JM. Isolation and functional characterization of the actin binding region in the tight junction protein ZO-1. FASEB J 2002;16:1835-7.
14. Fanning AS, Little BP, Rahner C, Utepbergenov D, Walther Z, Anderson JM. The unique-5 and -6 motifs of ZO-1 regulate tight junction strand localization and scaffolding properties. Mol Biol Cell 2007;18:721-31.
15. Feldman GJ, Mullin JM, Ryan MP. Occludin: structure, function and regulation. Adv Drug Deliv Rev 2005;57:883-917.
16. Ghassemifar R, Lai CM, Rakoczy PE. VEGF differentially regulates transcription and translation of ZO-1alpha+ and ZO-1alpha- and mediates trans-epithelial resistance in cultured endothelial and epithelial cells. Cell Tissue Res 2006;323:117-25.
17. Halbleib JM, Sääf AM, Brown PO, Nelson WJ. Transcriptional modulation of genes encoding structural characteristics of differentiating enterocytes during development of a polarized epithelium in vitro. Mol Biol Cell 2007;18:4261-78.
18. Ikenouchi J, Umeda K, Tsukita S, Furuse M, Tsukita S. Requirement of ZO-1 for the formation of belt-like adherens junctions during epithelial cell polarization. J Cell Biol 2007;176:779-86.
19. Itoh M, Nagafuchi A, Yonemura S, Kitani-Yasuda T, Tsukita S, Tsukita S. The 220-kD protein colocalizing with cadherins in non-epithelial cells is identical to ZO-1, a tight junction-associated protein in epithelial cells: cDNA cloning and immunoelectron microscopy. J Cell Biol 1993;121:491-502.
20. Kruse C, Birth M, Rohwedel J, Assmuth K, Goepel A, Wedel T. Pluripotency of adult stem cells derived from human and rat pancreas. Appl Phys A-Mater 2004;79:1617-24.
21. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970;227:680-5.
22. Matter K, Balda MS. Epithelial tight junctions, gene expression and nucleo-junctional interplay. J Cell Sci 2007;120:1505-11.
23. Meier K, Lehr CM, Daum N. Differentiation potential of human pancreatic stem cells for epithelial- and endothelial-like cell types. Ann Anat 2009;191:70-82.
24. - in guinea pig Sertoli cells: a possible association with Factin and G-actin. Biol Reprod 1997;57:367-76.
25. Piana C, Güll I, Gerbes S, Gerdes R, Mills C, Samitier J et al. Influence of surface modification on vitality and differentiation of Caco-2 cells. Differentiation 2007;75:308-17.
26. Piana C, Toegel S, Guell I, Gerbes S, Viernstein H, Wirth M et al. Growth surface-induced gene and protein expression patterns in Caco-2 cells. Acta Biomater 2008;4:1819-26.
27. Pinto M, Robine-Leon S, Appay MD, Kedinger M, Triadou N, Dussaulx E et al. Enterocyte-like differentiation and polarization of the human colon carcinoma cell line Caco-2 in culture. Biol Cell 1983;47:323-30.
28. Roschke AV, Stover K, Tonon G, Schäffer AA, Kirsch IR. Stable karyotypes in epithelial cancer cell lines despite high rates of ongoing structural and numerical chromosomal instability. Neoplasia 2002;4:19-31.
29. Sääf AM, Halbleib JM, Chen X, Yuen ST, Leung SY, Nelson WJ et al. Parallels between global transcriptional programs of polarizing Caco-2 intestinal epithelial cells in vitro and gene expression programs in normal colon and colon cancer. Mol Biol Cell 2007;18:4245-60.
30. Sambuy Y, De Angelis I, Ranaldi G, Scarino ML, Stammati A, Zucco F. The Caco-2 cell line as a model of the intestinal barrier: influence of cell and culture-related factors on Caco-2 cell functional characteristics. Cell Biol Toxicol 2005;21:1-26.
31. Sheth B, Fesenko I, Collins JE, Moran B, Wild AE, Anderson JM et al. Tight junction assembly during mouse blastocyst formation is regulated by late expression of ZO-1 alpha+ isoform. Development 1997;124:2027-37.
32. Stefanovic V, Vlahovic P, Ardaillou N, Ronco P, Ardaillou R. Cell surface aminopeptidase A and N activities in human glomerular epithelial cells. Kidney Int. 1992;41:1571-80.
33. Stö ber W, Fink A. Controlled growth of monodisperse silica spheres in the micron size range. J Colloid Interface Sci 1968;26:62-96.
34. Suda K, Rothen-Rutishauser B, Günthert M, Wunderli-Allenspach H. Phenotypic characterization of human umbilical vein endothelial (ECV304) and urinary carcinoma (T24) cells: endothelial versus epithelial features. In Vitro Cell Dev Biol Anim 2001;37:505-14.
35. Towbin H, Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 1979;76:4350-4.
36. Umeda K, Ikenouchi J, Katahira-Tayama S, Furuse K, Sasaki H, Nakayama M et al. ZO-1 and ZO-2 independently determine where claudins are polymerized in tight-junction strand formation. Cell 2006;126:741-54.
37. Umeda K, Matsui T, Nakayama M, Furuse K, Sasaki H, Furuse M et al. Establishment and characterization of cultured epithelial cells lacking expression of ZO-1. J Biol Chem 2004;279:44785-94.
38. Willott E, Balda MS, Heintzelman M, Jameson B, Anderson JM. Localization and differential expression of two isoforms of the tight junction protein ZO-1. Am J Physiol 1992;262:C1119-24.