Detection and quantification of the free-living stage of the parasitic dinoflagellate Hematodinium sp. in laboratory and environmental samples

Harmful Algae

Volumn 9, Issue 5, 2010, Pages 515-521

  Li, Caiwen ^a   Shields, Jeffrey D ^a   Miller, Terrence L ^a   Small, Hamish J ^a   Pagenkopp, Katrina M ^a   Reece, Kimberly S ^a  

^a VIRGINIA INSTITUTE OF MARINE SCIENCE   (United States)

Author keywords

Blue crab; Dinoflagellate; Dinospores; Hematodinium; Real time PCR

Indexed keywords

CALLINECTES SAPIDUS; CRUSTACEA; DECAPODA (CRUSTACEA); DINOPHYCEAE; HEMATODINIUM; HEMATODINIUM SP.;

EID: 77955058395     PISSN: 15689883     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.hal.2010.04.001     Document Type: Article

References (35)

1. Audemard C., Reece K.S., Burreson E.M. Real-time PCR for detection and quantification of the protistan parasite Perkinsus marinus in environmental waters. Appl. Environ. Microbiol. 2004, 70:6611-6618.
2. Bowers H.A., Tengs T., Glasgow H.B., Burkholder J.M., Rublee P.A., Oldach D.W. Development of real-time PCR assays for rapid detection of Pfiesteria piscicida and related dinoflagellates. Appl. Environ. Microbiol. 2000, 66:4641-4648.
3. Burkholder J.M., Azanza R.V., Sako Y. The ecology of harmful dinoflagellates. Ecology of Harmful Algae 2006, 53-66. Springer-Verlag, Berlin. E. Graneli, J.T. Turner (Eds.).
4. Chatton E., Poisson R. Sur l'existence, dans le sang des crabs, de peridiniens parasites: Hematodinium perezi n.g., n.sp. (Syndinidae). CR Sceances Soc. Biol. Paris 1931, 105:553-557.
5. Dungan C.F., Reece K.S. In vitro propagation of two Perkinsus spp. parasites from Japanese Manila clams. Venerupis philippinarum, and description of Perkinsus honshuensis n. sp. J. Eukaryot. Microbiol. 2006, 53:316-326.
6. Dyhrman S.T., Erdner D., Du J.L., Galac M., Anderson D.M. Molecular quantification of toxic Alexandrium fundyense in the Gulf of Maine using real-time PCR. Harmful Algae 2006, 5:242-250.
7. Eaton W.D., Love D.C., Botelho C., Meyers T.R., Imamura K., Koeneman T. Preliminary results on the seasonality and life cycle of the parasitic dinoflagellate causing bitter crab disease in Alaskan Tanner Crabs (Chionoecetes bairdi). J. Invertebr. Pathol. 1991, 57:426-434.
8. Frischer M.E., Lee R.F., Sheppard M.A., Mauer A., Rambow R., Neumann M., Brofft J.E., Wizenmann T., Danforth J.M. Evidence for a free-living life stage of the blue crab parasitic dinoflagellate, Hematodinium sp. Harmful Algae 2006, 5:548-557.
9. Frostegard A., Courtois S., Ramisse V., Clerc S., Bernillon D., Le Gall F., Jeannin P., Nesme X., Simonet P. Quantification of bias to the extraction of DNA directly from soils. Appl. Environ. Microbiol. 1999, 65:5409-5420.
10. Galluzzi L., Penna A., Bertozzini E., Vila M., Garces E., Magnani M. Development of a Real-time PCR assay for rapid detection and quantification of Alexandrium minutum (a dinoflagellate). Appl. Environ. Microbiol. 2004, 70:1199-1206.
11. Godhe A., Asplund M.E., Härnström K., Saravanan V., Tyagi A., Karunasagar I. Quantification of diatom and dinoflagellate biomasses in coastal marine seawater samples by Real-time PCR. Appl. Environ. Microbiol. 2008, 74:7174-7182.
12. Gray M., Wawrik B., Paul J., Casper E. Molecular detection and quantification of the red tide dinoflagellate Karenia brevis in the marine environment. Appl. Environ. Microbiol. 2003, 69:5726-5730.
13. Gruebl T., Frischer M.E., Sheppard M., Neuman M., Maurer A.N., Lee R.F. Development of an 18S rRNA gene targeted PCR based diagnostic for the blue crab parasite Hematodinium sp. Dis. Aquat. Org. 2002, 49:61-70.
14. Handy S.M., Demir E., Hutchins D.A., Portune K.J., Whereat E.B., Hare C.E., Rose J.M., Warner M., Farestad M., Cary S.C., Coyne K.J. Using quantitative real-time PCR to study competition and community dynamics among Delaware Inland Bays harmful algae in field and laboratory studies. Harmful Algae 2008, 7:599-613.
15. Hein I., Lehner A., Rieck P., Klein K., Brandl E., Wagner M. Comparison of different approaches to quantify Staphylococcus aureus cells by real-time PCR and application of this technique for examination of cheeses. Appl. Environ. Microbiol. 2001, 67:3122-3126.
16. Hudson D.A., Adlard R.D. PCR-techniques applied to Hematodinium spp. and Hematodinium-like dinoflagellates in decapod crustaceans. Dis. Aquat. Org. 1994, 20:203-206.
17. Hudson D.A., Adlard R.D. Nucleotide sequence determination of the partial SSU rDNA gene and ITS1 region of Hematodinium cf. Perezi and Hematodinium-like dinoflagellates. Dis. Aquat. Org. 1996, 24:55-60.
18. Johnson P.T. Parasites of benthic amphipods: dinoflagellates (Duboscquodinida: Syndinidae). Fish Bull. 1986, 84:605-614.
19. Kreader C.A. Relief of amplification inhibition in PCR with bovine serum albumin or T4 Gene 32 protein. Appl. Environ. Microbiol. 1996, 62:1102-1106.
20. Li C., Shields J.D., Small H.J., Reece K.S., Hartwig C.L., Cooper R.A., Ratzlaff R.E. Detection of Panulirus argus Virus 1 (PaV1) in the Caribbean spiny lobster using fluorescence in situ hybridization (FISH). Dis. Aquat. Org. 2006, 72:185-192.
21. Messick G.A. Hematodinium perezi infections in adult and juvenile blue crabs Callinectes sapidus from coastal bays of Maryland and Virginia, USA. Dis. Aquat. Org. 1994, 19:77-82.
22. Messick G.A., Jordan S.J., Van Heukelem W.F. Salinity and temperature effects on Hematodinium sp. in the blue crab Callinectes sapidus. J. Shellfish Res. 1999, 18:657-662.
23. Messick G.A., Shields J.D. Epizootiology of the parasitic dinoflagellate Hematodinium sp. in the American blue crab Callinectes sapidus. Dis. Aquat. Org. 2000, 43:139-152.
24. Meyers T.R., Koeneman T.M., Bothelho C., Short S. Bitter crab disease: a fatal dinoflagellate infection and marketing problem for Alaskan Tanner crabs Chionoecetes bairdii. Dis. Aquat. Org. 1987, 3:195-216.
25. Nagle L., Place A.R., Schott E.J., Jagus R., Messick G., Pitula J.S. Real-time PCR-based assay for quantitative detection of Hematodinium sp. in the blue crab Callinectes sapidus. Dis. Aquat. Org. 2009, 84:79-87.
26. Newman M.W., Johnson C.A. A disease of blue crabs (Callinectes sapidus) caused by a parasitic dinoflagellate, Hematodinium sp. J. Parasitol. 1975, 63:554-557.
27. Park T.G., Salas M.F., Bolch C.J.S., Hallegraeff G.M. Development of a real-time PCR probe for quantification of the heterotrophic dinoflagellate Cryptoperidiniopsis brodyi (Dinophyceae) in environmental samples. Appl. Environ. Microbiol. 2007, 73:2552-2560.
28. Sheppard M., Walker A., Frischer M.E., Lee R.F. Histopathology and prevalence of the parasitic dinoflagellate Hematodinium sp., in crabs (Callinectes sapidus, Callinectes similis, Neopanope sayi. Libiniae marginata, Menippemer cenaria) from a Georgia estuary. J. Shellfish Res. 2003, 22:873-880.
29. Shields J.D. The parasitic dinoflagellates of marine crustaceans. Annu. Rev. Fish. Dis. 1994, 4:241-271.
30. Shields J.D., Squyars C.M. Mortality and hematology of blue crabs. Callinectes sapidus, experimentally infected with the parasitic dinoflagellate Hematodinium perezi. Fish Bull. 2000, 98:139-152.
31. Shields J.D., Taylor D.M., Sutton S.G., O'Keefe P.G., Ings D.W., Pardy A.L. Epidemiology of bitter crab disease (Hematodinium sp.) in snow crabs Chionoecetes opilio from Newfoundland. Can. Dis. Aquat. Org. 2005, 64:253-264.
32. Small H.J., Shields J.D., Hudson K.L., Reece K.S. Molecular detection of Hematodinium sp. infecting the blue crab Callinectes sapidus. J. Shellfish Res. 2007, 26:131-139.
33. Stentiford G.D., Shields J.D. A review of the parasitic dinoflagellates Hematodinium species and Hematodinium-like infections in marine crustaceans. Dis. Aquat. Org. 2005, 66:47-70.
34. Walker A.N., Lee R.F., Frischer M.E. Transmission of the parasitic dinoflagellate Hematodinium sp. infection in blue crabs Callinectes sapidus by cannibalism. Dis. Aquat. Org. 2009, 85:193-197.
35. Watson R.J., Blackwell B. Purification and characterization of a common soil component which inhibits the polymerase chain reaction. Can. J. Microbiol. 2000, 46:633-642.