Comparison of fermentation of diets of variable composition and microbial populations in the rumen of sheep and Rusitec fermenters. II. Protozoa population and diversity of bacterial communities

Journal of Dairy Science

Volumn 93, Issue 8, 2010, Pages 3699-3712

  Martinez M E ^a,b   Ranilla, M J ^a,b   Tejido, M L ^a,b   Saro, C ^a,b   Carro, M D ^a,b  

^a UNIVERSITY OF LEÓN   (Spain)

^b Instituto de Ganadería de Montana   (Spain)

Author keywords

Bacterial diversity; Protozoa; Rusitec; Sheep

Indexed keywords

ANIMAL; ANIMAL FOOD; ARTICLE; BACTERIUM; BIODIVERSITY; CLASSIFICATION; COMPARATIVE STUDY; FERMENTATION; MICROBIOLOGY; PARASITOLOGY; PHYSIOLOGY; RUMINANT STOMACH; SHEEP; BACTERIA;

ANIMAL FEED; ANIMAL NUTRITIONAL PHYSIOLOGICAL PHENOMENA; ANIMALS; BACTERIA; BIODIVERSITY; FERMENTATION; RUMEN; SHEEP;

BACTERIA (MICROORGANISMS); ISOTRICHIDAE; MEDICAGO SATIVA; OVIS ARIES; PROTOZOA;

EID: 77955032141     PISSN: 00220302     EISSN: 15253198     Source Type: Journal    
DOI: 10.3168/jds.2009-2934     Document Type: Article

References (55)

1. Abe M., Kurihara Y. Long-term cultivation of certain rumen protozoa in a continuous fermentation system supplemented with sponge materials. J. Appl. Bacteriol. 1984, 56:201-203.
2. Boguhn J., Strobel E., Witziga M., Tebbec C.C., Rodehutscord M. Description of the structural diversity of rumen microbial communities in vitro using single-strand conformation polymorphism profiles. Arch. Anim. Nutr. 2008, 62:454-467.
3. Brossard L., Martin C., Michalet-Doreau B. Ruminal fermentative parameters and blood acid-basic balance changes during the onset and recovery of induced latent acidosis in sheep. Anim. Res. 2003, 52:513-530.
4. Carro M.D., Lebzien P., Rohr K. Influence of yeast culture on the in vitro fermentation (Rusitec) of diets containing variable portions of concentrates. Anim. Feed Sci. Technol. 1992, 37:209-220.
5. Carro M.D., Lebzien P., Rohr K. Effects of pore size of nylon bags and dilution rate parameters in a semi-continuous artificial rumen. Small Rumin. Res. 1995, 15:113-119.
6. 15N and purine bases) and bacterial isolates for the estimation of rumen microbial protein synthesis. Anim. Sci. 2002, 75:315-321.
7. Crawford R.J., Hoover W.H., Knowlton P.H. Effects of solids and liquid flows on fermentation in continuous cultures. I. Dry matter and fiber digestion, VFA production and protozoa numbers. J. Anim. Sci. 1980, 51:975-985.
8. Czerkawski J.W., Breckenridge G. Design and development of a long-term rumen simulation technique (Rusitec). Br. J. Nutr. 1977, 38:371-384.
9. Danovaro R., Luna G.M., Dell'Anno A., Pietrangeli B. Comparison of two fingerprinting techniques, terminal restriction fragment length polymorphism and automated ribosomal intergenic spacer analysis, for determination of bacterial diversity in aquatic environments. Appl. Environ. Microbiol. 2006, 72:5982-5989.
10. Dehority B.A. Pectin-fermenting bacteria isolated from the bovine rumen. J. Bacteriol. 1969, 99:189-196.
11. Dehority B.A. Laboratory Manual for Classification and Morphology of Ruminal Ciliate Protozoa 1993, CRC Press, Boca Raton, FL.
12. Dehority B.A. Rumen Microbiology 2003, Nottingham Univ. Press, Nottingham, UK.
13. Dehority B.A., Tirabasso P.A., Grifo A.P. Most-probable-number procedures for enumerating ruminal bacteria, including the simultaneous estimation of total bacteria and cellulolytic numbers in one medium. Appl. Environ. Microbiol. 1989, 55:2789-2792.
14. Denman S.E., Nicholson M.J., Brookman J.L., Theodorou M.K., McSweeney C.S. Detection and monitoring of anaerobic rumen fungi using an ARISA method. Lett. Appl. Microbiol. 2008, 47:492-499.
15. Eadie J.M. The development of rumen microbial populations in lambs and calves under various conditions of management. J. Gen. Microbiol. 1962, 29:563-578.
16. Firkins J.L., Yu Z. Characterisation and quantification of the microbial populations of the rumen. Ruminant, Digestion, Metabolism and Impact on Nutrition on Gene Expression, Immunology and Stress 2006, 19-54. Wageningen Academic Publishers, Wageningen, the Netherlands. K. Serjsen, T. Hevelplund, M.O. Nielsen (Eds.).
17. Fisher M.M., Triplett E.W. Automated approach for ribosomal intergenic spacer analysis of microbial diversity and its application to freshwater bacterial communities. Appl. Environ. Microbiol. 1999, 65:4630-4636.
18. Franzolin R., Dehority B.A. Effect of prolonged high-concentrate feeding on ruminal protozoa concentrations. J. Anim. Sci. 1996, 74:2803-2809.
19. Gizzi G., Zanchi R., Sciaraffia F. Comparison of microbiological and fermentation parameters obtained with an improved rumen in vitro technique with those obtained in vivo. Anim. Feed Sci. Technol. 1998, 73:291-305.
20. Hristov A.N., Ivan M., Rode L.M., McAllister T.A. Fermentation characteristics and ruminal ciliate protozoal populations in cattle fed medium- or high-concentrate barley-based diets. J. Anim. Sci. 2001, 79:515-524.
21. Ipharraguerre I.R., Reynal S.M., Liñeiro M., Broderick G.A., Clark J.H. A comparison of sampling sites, digesta and microbial markers, and microbial references for assessing the postruminal supply of nutrients in dairy cows. J. Dairy Sci. 2007, 90:1904-1919.
22. Kocherginskaya S.A., Aminov R.I., White B.A. Analysis of the rumen bacterial diversity under two different diet conditions using denaturing gradient gel electrophoresis, random sequencing, and statistical ecology approaches. Anaerobe 2001, 7:119-134.
23. Koike S., Pan J., Kobayashi Y., Tanaka K. Kinetics of in sacco fiber attachment of representative ruminal cellulolytic bacteria monitored by competitive PCR. J. Dairy Sci. 2003, 86:1429-1435.
24. Larue R., Yu Z., Parisi V.A., Egan A.R., Morrison M. Novel microbial diversity adherent to plant biomass in the herbivore gastrointestinal tract, as revealed by ribosomal intergenic spacer analysis and rrs gene sequencing. Environ. Microbiol. 2005, 74:530-543.
25. Leedle J.A.Z., Barshun K., Hespell R.B. Postprandial trends in estimated ruminal digesta polysaccharides and their relation to changes in bacterial groups and ruminal fluid characteristics. J. Anim. Sci. 1986, 62:789-803.
26. Mackie R.I., Gilchrist F.M.C., Roberts A.M., Hannah P.E., Schwartz H.M. Microbiological and chemical changes in the rumen during the stepwise adaptation of sheep to high concentrate diets. J. Agric. Sci. 1978, 90:241-254.
27. Mackie R.I., Sghir A., Gaskins H.R. Developmental microbial ecology of the neonatal gastrointestinal tract. Am. J. Clin. Nutr. 1999, 69:1035S-1045S.
28. Mansfield H.R., Endres M.I., Stern M.D. Comparison of microbial fermentation in the rumen of dairy cows and dual flow continuous culture. Anim. Feed Sci. Technol. 1995, 55:47-66.
29. Martin C., Millet L., Fonty G., Michalet-Doreau B. Cereal supplementation modified the fibrolytic activity but not the structure of the cellulolytic bacterial community associated with rumen solid digesta. Reprod. Nutr. Dev. 2001, 41:413-424.
30. Martínez M.E., Ranilla M.J., Ramos S., Tejido M.L., Carro M.D. Effects of dilution rate and retention time of concentrate on efficiency of microbial growth, methane production, and ruminal fermentation in Rusitec fermenters. J. Dairy Sci. 2009, 92:3930-3938.
31. Martínez M.E., Ranilla M.J., Ramos S., Tejido M.L., Saro C., Carro M.D. Evaluation of procedures for detaching particle-associated microbes from forage and concentrate incubated in Rusitec fermenters: Efficiency of recovery and representativeness of microbial isolates. J. Anim. Sci. 2009, 87:2064-2072.
32. Martínez M.E., Ranilla M.J., Tejido M.L., Saro C., Carro M.D. Comparison of fermentation of diets of variable composition in the rumen of sheep and Rusitec fermenters. I. Digestibility, fermentation parameters, and efficiency of microbial protein synthesis. J. Dairy Sci. 2010, 93:3684-3698.
33. McDougall E.I. Studies on ruminant saliva. I. The composition and output of sheep's saliva. Biochem. J. 1948, 43:99-109.
34. Merry R.J., McAllan A.B. A comparison of the chemical composition of mixed bacteria harvested from the liquid and solid fractions of rumen digesta. Br. J. Nutr. 1983, 50:701-709.
35. Michalet-Doreau B., Fernandez I., Peyron C., Millet L., Fonty G. Fibrolytic activities and cellulolytic bacterial community structure in the solid and liquid phases of rumen contents. Reprod. Nutr. Dev. 2001, 41:187-194.
36. Molina-Alcaide E., Moumen A., Martín García I., Carro M.D. Comparison of bacterial pellets and microbial markers for the estimation of the microbial nitrogen and amino acids flows from single flow continuous culture fermenters fed diets containing two-stage olive cake. J. Anim. Physiol. Anim. Nutr. (Berl.) 2009, 93:527-537.
37. Muetzel S., Lawrence P., Hoffmann E.M., Becker K. Evaluation of a stratified continuous rumen incubation system. Anim. Feed Sci. Technol. 2009, 151:32-43.
38. Nakamura F., Kurihara Y. Maintenance of a certain rumen protozoal population in a continuous in vitro fermentation system. Appl. Environ. Microbiol. 1978, 35:500-506.
39. Nutrient Requirements of Sheep 1985, NRC, National Academy Press, Washington, DC. 6th ed.
40. Ogimoto K., Imai S. Atlas of Rumen Microbiology 1981, Scientific Societies Press, Tokyo, Japan.
41. Prevot S., Senaud J., Prensier G. Variation in the composition of the ruminal bacterial microflora during the adaptation phase in an artificial fermenter (Rusitec). Zoolog. Sci. 1994, 11:871-882.
42. Ramos S., Tejido M.L., Ranilla M.J., Martínez M.E., Saro C., Carro M.D. Influence of detachment procedure and diet on recovery of solid-associated bacteria from sheep ruminal digesta and representativeness of bacterial isolates as assessed by automated ribosomal intergenic spacer analysis-polymerase chain reaction. J. Dairy Sci. 2009, 92:5659-5668.
43. Ranilla M.J., Carro M.D. Diet and procedures used to detach particulate-associated microbes from ruminal digesta influence chemical composition of microbes and estimation of microbial growth in Rusitec fermenters. J. Anim. Sci. 2003, 81:537-544.
44. Roger V., Fonty G., Komisarczuk-Bony S., Gouet P. Effects of physicochemical factors on the adhesion to cellulose Avicel of the ruminal bacteria Ruminococcus flavefaciens and Fibrobacter succinogenes ssp. succinogenes. Appl. Environ. Microbiol. 1990, 56:3081-3087.
45. Santra A., Karim S.A., Mishra A.S., Chaturvedi O.H., Prasad R. Rumen ciliate protozoa and fiber digestion in sheep and goats. Small Rumin. Res. 1998, 30:13-18.
46. Shannon C.E., Weaver W. The Mathematical Theory of Communication 1949, University of Illinois Press, Urbana.
47. Slyter L.L., Putnam P.A. In vivo vs. in vitro continuous culture of ruminal microbial populations. J. Anim. Sci. 1967, 26:1421-1427.
48. Spanish Government. 2005. Royal Decree 1201/2005 for the protection of animals used for experimental and other scientific purposes of October 10th, BOE 252. Pages 34367-34274.
49. Stewart C.S., Flint H.J., Bryant M.P. The rumen bacteria. The Rumen Microbial Ecosystem 1997, 10-72. Chapman and Hall, London, UK. P.N. Hobson, C.S. Stewart (Eds.).
50. Sylvester J.T., Karnati S.K.R., Dehority B.A., Morrison M., Smith G.L., St-Pierre N.R., Firkins J.L. Rumen ciliated protozoa decrease generation time and adjust 18S ribosomal DNA copies to adapt to decreased transfer interval, starvation, and monensin. J. Dairy Sci. 2009, 92:256-269.
51. Teather R.M., Sauer F.D. A naturally compartmented rumen simulation system for the continuous culture of rumen bacteria and protozoa. J. Dairy Sci. 1988, 71:666-673.
52. Welkie D.G., Stevenson D.M., Weimer P.J. ARISA analysis of ruminal bacterial community dynamics in lactating dairy cows during the feeding cycle. Anaerobe 2010, 16:94-100.
53. Williams P.P., Davis R.E., Doetsch R.N., Guiterrez J. Physiological studies of the rumen protozoan Ophryoscolex caudatus Eberlein. Appl. Microbiol. 1961, 9:405-409.
54. Yu Z., Morrison M. Improved extraction of PCR-quality community DNA from digesta and fecal samples. Biotechniques 2004, 36:808-812.
55. Ziemer C.J., Sharp R., Stern M.D., Cotta M.A., Whitehead T.R., Stahl D.A. Comparison of microbial populations in model and natural rumens using 16S ribosomal RNA-targeted probes. Environ. Microbiol. 2000, 1:632-643.