Differential production of tropane alkaloids in hairy roots and in vitro cultured two accessions of Atropa belladonna L. under nitrate treatments

Zeitschrift fur Naturforschung - Section C Journal of Biosciences

Volumn 65 C, Issue 5-6, 2010, Pages 373-379

  Chashmi, Najmeh Ahmadian ^a   Sharifi, Mozafar ^a   Karimi, Farah ^b   Rahnama, Hasan ^c  

^a TARBIAT MODARES UNIVERSITY   (Iran)

^b SHAHED UNIVERSITY   (Iran)

^c AGRICULTURAL BIOTECHNOLOGY RESEARCH INSTITUTE OF IRAN   (Iran)

Author keywords

Agrobacterium rhizogenes; Atropa belladonna; Hairy roots; Tropane alkaloids

Indexed keywords

EID: 77954966097     PISSN: 09395075     EISSN: None     Source Type: Journal    
DOI: 10.1515/znc-2010-5-609     Document Type: Article

References (25)

1. Arnon D. I. (1949), Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiol. 24, 1-15.
2. Bensaddek L., Gillet F., Nava Saucedo J. E., and Fliniaux M. (2001), The effect of nitrate and ammonium concentrations on growth and alkaloid accumulation of Atropa belladonna hairy roots. J. Biotechnol. 85, 35-40.
3. Bonhomme V., Laurain-Mattar D., Lacoux J., Fliniaux M., and Jacquin-Dubreuil A. (2000), Tropane alkaloid production by hairy roots of Atropa belladonna obtained after transformation with Agrobacterium rhizogenes 15834 and Agrobacterium tumefaciens containing rol A, B, C genes only. J. Biotechnol. 81, 151-158.
4. Dashek W. V. (1997), Methods in Plants Biochemistry and Molecular Biology. CRC Press, Boca Raton, USA, pp. 185-189.
5. Dechaux C. and Boitel-Conti M. (2005), A strategy for overaccumulation of scopolamine in Datura innoxia hairy root cultures. Acta Biol. Cracov. Ser. Bot. 47/1, 101-107.
6. +) on alkaloid production in Datura stramonium L. Plant Soil 147, 79-86.
7. Dupraz J., Christen P., and Kapetanidis I. (1993), Tropane alkaloids in transformed roots of Datura quercifolia. Planta Med. 60, 158-162.
8. Hashimoto T. and Yamada Y. (1983), Scopolamine production in suspension cultures and redifferentiated roots of Hyoscyamus niger. Planta Med. 47, 121-141.
9. Hashimoto T. and Yamada Y. (1986), Hyoscyamine 6β-hydroxylase, a 2-oxoglutarate-dependent dioxygenase, in alkaloid-producing root cultures. Plant Physiol. 81, 619-625.
10. Hashimoto T., Hayashi A., Amano Y., Kohno J., Iwanari H., Usuda S., and Yamada Y. (1991), Hyoscyamine 6β-hydroxylase, an enzyme involved in tropane alkaloid biosynthesis, is localized at the pericycle of the root. J. Biol. Chem. 266/7, 4648-4653.
11. Khan S., Irfan Qureshi M., Kamaluddin Alam T., and Abdin M. Z. (2007), Protocol for isolation of genomic DNA from dry and fresh roots of medicinal plants suitable for RAPD and restriction digestion. Afr. J. Biotechnol. 6, 175-178.
12. Kim Y., Wyslouzil E. B., and Pamela J. (2002), Secondary metabolism of hairy root cultures in bioreactors. In vitro Cell. Dev. Biol. Plant. 38, 1-10.
13. Krizek D. T., Brita S. J., and Miewcki R. M. (1998), Inhibitory effects of ambient levels of solar UV-A and UV-B on growth of cv. New Red Fire lettuce. Physiol. Plant. 103, 1-7.
14. Mantell S. H., Pearson D. W., Hazell L. P., and Smith H. (1983), The effect of initial phosphate and sucrose levels on nicotine accumulation in batch suspension cultures of Nicotiana tabacum. Plant Cell Rep. 2, 73-77.
15. Mateus L., Cherkaoui S., Christen P., and Veuthey J. (2000), Enantioseparation of atropine by capillary electrophoresis using sulfated b-cyclodextrin: application to a plant extract. J. Chromatogr. A 868, 285-294.
16. Mendoza M. I. E. and Vargas L. V. M. (1995), Establishment and characterization of photosynthetic hairy root cultures of Datura stramonium. Plant Cell Tiss. Organ Cult. 40, 197-208.
17. Murashige T. and Skoog F. (1962), A revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant Physiol. 15, 473-497.
18. Palazon J., Moyano E., Cusidó R. M., Bonfill M., Oksman-Caldentey K. M., and Pinol M. T. (2003), Alkaloid production in Duboisia hybrid hairy roots and plants overexpressing the h6h gene. Plant Sci. 165, 1289-1295.
19. Payne J., Hamill J. D., Robins R. J., and Rhodes J. C. (1987), Production of hyoscyamine by hairy root cultures of Datura stramonium. Planta Med. 53, 474-478.
20. Pinol M. T., Palazon J., Cusido M. R., and Ribo M. (1999), Influence of calcium ion-concentration in the medium on tropane alkaloid accumulation in Datura stramonium hairy roots. Plant Sci. 141, 41-49.
21. Roos R. W. and Lau-Cam C. (1986), General reversedphase HPLC method for the separation of drugs using triethylamine as a competing base. J. Chromatogr. 370, 403-418.
22. Rothe G., Hachiya A., Yamada Y., Hashimoto T., and Èger B. (2003), Alkaloids in plants and root cultures of Atropa belladonna overexpressing putrescine Nmethyltransferase. J. Exp. Bot. 54, 2065-2070.
23. Takizawa M., Hori K., Inai K., Takase H., Hashimoto T., and Watanabe Y. (2007), A virus-induced gene silencing approach for the suppression of nicotine content in Nicotiana benthamiana. Plant Biotechnol. 24, 295-300.
24. Wang B., Zhang G., Zhu L., Chen L., and Zhang Y. (2006), Genetic transformation of Echinacea purpurea with Agrobacterium rhizogenes and bioactive ingredient analysis in transformed cultures. Colloids Surf. B 53, 101-104.
25. Yamamoto O. and Kamura K. (1997), Production of saikosaponin in cultured roots of Bupleurum falcatum L. Plant Tissue Cult. Biotechnol. 3, 138-147.