Recombinant Mycobacterium bovis BCG expressing chimaeric protein of Ag85B and ESAT-6 enhances immunostimulatory activity of human macrophages

Microbes and Infection

Volumn 12, Issue 8-9, 2010, Pages 683-689

  Xu, Ying ^a   Liu, Wei ^a   Shen, Hongbo ^a   Yan, Jingran ^a   Yang, Enzhuo ^a   Wang, Honghai ^a  

^a FUDAN UNIVERSITY   (China)

Author keywords

Ag85B; ESAT 6; M. tuberculosis; Macrophage; Recombinant BCG

Indexed keywords

ACTIN BETA; ACTIN GAMMA 1 PROPEPTIDE; ANTIGEN 85B; B7 ANTIGEN; BCG VACCINE; CD40 ANTIGEN; CD86 ANTIGEN; CELL PROTEIN; CHIMERIC PROTEIN; EARLY SECRETORY ANTIGENIC TARGET 6; HLA DR ANTIGEN; PROTEIN EF HAND DOMAIN FAMILY MEMBER D2; RECOMBINANT VACCINE; TUMOR NECROSIS FACTOR ALPHA; UNCLASSIFIED DRUG; ACTIN; ACYLTRANSFERASE; ANTIGEN 85B, MYCOBACTERIUM TUBERCULOSIS; BACTERIAL ANTIGEN; BACTERIAL PROTEIN; CALCIUM BINDING PROTEIN; EFHD2 PROTEIN, HUMAN; ESAT-6 PROTEIN, MYCOBACTERIUM TUBERCULOSIS; LEUKOCYTE ANTIGEN; RECOMBINANT PROTEIN;

ANTIGEN PRESENTATION; ARTICLE; CD8+ T LYMPHOCYTE; CHILD; CONTROLLED STUDY; CYTOKINE PRODUCTION; HUMAN; HUMAN CELL; IMMUNE RESPONSE; IMMUNOSTIMULATION; IN VITRO STUDY; INFANT; MACROPHAGE; MYCOBACTERIUM BOVIS; NONHUMAN; PRESCHOOL CHILD; PRIORITY JOURNAL; PROTEIN EXPRESSION; TH1 CELL; UPREGULATION; BIOSYNTHESIS; CELL LINE; GENE EXPRESSION PROFILING; GENETICS; IMMUNOLOGY; MICROBIOLOGY; TWO DIMENSIONAL GEL ELECTROPHORESIS;

ACTINS; ACYLTRANSFERASES; ANTIGENS, BACTERIAL; ANTIGENS, CD; BACTERIAL PROTEINS; CALCIUM-BINDING PROTEINS; CELL LINE; ELECTROPHORESIS, GEL, TWO-DIMENSIONAL; GENE EXPRESSION PROFILING; HLA-DR ANTIGENS; HUMANS; MACROPHAGES; MYCOBACTERIUM BOVIS; RECOMBINANT PROTEINS; TUBERCULOSIS VACCINES; TUMOR NECROSIS FACTOR-ALPHA;

MUS; MYCOBACTERIUM BOVIS BCG; MYCOBACTERIUM TUBERCULOSIS;

EID: 77954951890     PISSN: 12864579     EISSN: 1769714X     Source Type: Journal    
DOI: 10.1016/j.micinf.2010.04.002     Document Type: Article

References (27)

1. Skeiky Y.A.W., Sadoff J.C. Advances in tuberculosis vaccine strategies. Nat. Rev. Microbiol. 2006, 4:469-476.
2. Aagaard C., Dietrich J., Doherty M., Andersen P. TB vaccines: current status and future perspectives. Immunol. Cell Biol. 2009, 87:279-286.
3. Eddine A.N., Kaufmann S.H.E. Improved protection by recombinant BCG. Microbes Infect. 2005, 7:939-946.
4. Brandt L., Elhay M., Rosenkrands I., Lindblad E.B., Andersen P. ESAT-6 subunit vaccination against Mycobacterium tuberculosis. Infect. Immun. 2000, 68:791-795.
5. Olsen A.W., van Pinxteren L.A.H., Okkels L.M., Rasmussen P.B., Andersen P. Protection of mice with a tuberculosis subunit vaccine based on a fusion protein of antigen 85B and ESAT-6. Infect. Immun. 2001, 69:2773-2778.
6. Palendira U., Spratt J.M., Britton W.J., Triccas J.A. Expanding the antigenic repertoire of BCG improves protective efficacy against aerosol Mycobacterium tuberculosis infection. Vaccine 2005, 23:1680-1685.
7. Xu Y., Liu W., Shen H., Yan J., Qu D., Wang H. Recombinant Mycobacterium bovis BCG expressing the chimeric protein of antigen 85B and ESAT-6 enhances the Th1 cell-mediated response. Clin. Vaccine Immunol. 2009, 16:1121-1126.
8. Pieters J. Mycobacterium tuberculosis and the macrophage: maintaining a balance. Cell Host Microbe 2008, 3:339-407.
9. Yuan Y., Zhu Y., Crane D.D., Barry C.E. The effect of oxygenated mycolic acid composition on cell wall function and macrophage growth in Mycobacterium tuberculosis. Mol. Microbiol. 1998, 29:1449-1458.
10. Zheng L., He M., Long M., Blomgran R., Stendahl O. Pathogeninduced apoptotic neutrophils express heat shock proteins and elicit activation of human macrophages. J. Immunol. 2004, 173:6319-6326.
11. Scheler C., Lamer S., Pan Z., Li X.P., Salnikow J., Jungblut P. Peptide mass fingerprint sequence coverage from differentially stained proteins on 2-DE patterns by MALDI-MS. Electrophoresis 1998, 19:918-927.
12. Vuadens F., Rufer N., Kress A., Corthésy P., Schneider P., Tissot J. Identification of swiprosin 1 in human lymphocytes. Proteomics 2004, 4:2216-2220.
13. Erba H.P., Eddy R., Shows T., Kedes L., Gunning P. Structure, chromosome location and expression of the human gamma-actin gene: differential evolution, location and expression of the cytoskeletal beta- and gamma-actin genes. Mol. Cell. Biol. 1988, 8:1775-1789.
14. Fonta'n P., Aris V., Ghanny S., Soteropoulos P., Smith I. Global transcriptional profile of Mycobacterium tuberculosis during THP-1 human macrophage infection. Infect. Immun. 2008, 76:717-725.
15. Kaufmann S.H.E. How can immunology contribute to the control of tuberculosis?. Nat. Rev. Microbiol. 2001, 1:20-30.
16. Sutherland J.S., Adetifa I.M., Hill P.C., Adegbola R.A., Ota M.O.C. Pattern and diversity of cytokine production differentiates between Mycobacterium tuberculosis infection and disease. Eur. J. Immunol. 2009, 39:723-729.
17. Feng Y., Yang X., Liu Z., Liu Y., Su B., Ding Y., Qin L., Yang H., Zheng R., Hu Z. Continuous treatment with recombinant Mycobacterium tuberculosis CFP-10-ESAT-6 protein activated human monocyte while deactivated LPS-stimulated macrophage. Biochem. Biophys. Res. Commun. 2008, 365:534-540.
18. Hwang S., Kruzel M.L., Actor J.K. Influence of bovine lactoferrin on expression of presentation molecules on BCG-infected bone marrow derived macrophages. Biochimie 2009, 91:76-85.
19. Pentcheva-Hoang T., Egen J.G., Wojnoonski K., Allison J.P. B7-1 and B7-2 selectively recruit CTLA-4 and CD28 to the immunological synapse. Immunity 2004, 21:401-413.
20. Bhatia S., Edidin M., Almo S.C., Nathenson S.G. B7-1 and B7-2: similar costimulatory ligands with different biochemical, oligomeric and signaling properties. Immunol. Lett. 2006, 104:70-75.
21. Spertini F., Chatila T., Geha R.S. Signals delivered via MHC class II molecules synergize with signals delivered via TCR/CD3 to cause proliferation and cytokine gene expression in T cells. J. Immunol. 1992, 49:65-70.
22. Makino M., Maeda Y., Inagaki K. Immunostimulatory activity of recombinant Mycobacterium bovis BCG that secretes major membrane protein II of Mycobacterium leprae. Infect. Immun. 2006, 74:6264-6271.
23. Darrah P.A., Patel D.T., De Luca P.M., Lindsay R.W.B., et al. Multifunctional TH1 cells define a correlate of vaccine mediated protection against Leishmania major. Nat. Med. 2007, 13:843-850.
24. Turner J., Dockrell H.M. Stimulation of human peripheral blood mononuclear cells with live Mycobacterium bovis BCG activates cytolytic CD8+ T cells in vitro. Immunology 1996, 87:339-342.
25. van Pinxteren L.A., Cassidy J.P., Smedegaard B.H., Agger E.M., Andersen P. Control of latent Mycobacterium tuberculosis infection is dependent on CD8 T cells. Eur. J. Immunol. 2000, 30:3689-3698.
26. Matsumoto H., Suzuki K., Tsuyuguchi K., Tanaka E., Amitani R., Maeda A., Yamamoto K., Sasada M., Kuze F. Interleukin-12 gene expression in human monocyte-derived macrophages stimulated with Mycobacterium bovis BCG: cytokine regulation and effect of NK cells. Infect. Immun. 1997, 65:4405-4410.
27. Xu Y., Wang B., Chen J., Wang Q., Zhu B., Shen H., Qie Y., Wang J., Wang H. Chimeric protein improved immunogenicity compared with fusion protein of Ag85B and ESAT-6 antigens of Mycobacterium tuberculosis. Scand. J. Immunol. 2006, 64:476-481.