Tracking differentiating neural progenitors in pluripotent cultures using microRNA-regulated lentiviral vectors

Proceedings of the National Academy of Sciences of the United States of America

Volumn 107, Issue 25, 2010, Pages 11602-11607

  Sachdeva, Rohit ^a   Jönsson, Marie E ^a   Nelander, Jenny ^a   Kirkeby, Agnete ^a   Guibentif, Carolina ^a   Gentner, Bernhard ^b   Naldini, Luigi ^b   Björklund, Anders ^a   Parmar, Malin ^a   Jakobsson, Johan ^a  

^a LUND UNIVERSITY   (Sweden)

^b VITA SALUTE SAN RAFFAELE UNIVERSITY   (Italy)

Author keywords

ES; Induced pluripotent stem cells; Stem cells; Transplantation

Indexed keywords

GREEN FLUORESCENT PROTEIN; LENTIVIRUS VECTOR; MICRORNA;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; CELL CULTURE; CELL LINEAGE; CELL SELECTION; HUMAN; HUMAN CELL; MOUSE; NERVE CELL DIFFERENTIATION; NEURAL STEM CELL; NEWBORN; NONHUMAN; PLURIPOTENT STEM CELL; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; STEM CELL TRANSPLANTATION; TRANSGENICS; ANIMAL; CELL DIFFERENTIATION; CELL SEPARATION; CULTURE TECHNIQUE; CYTOLOGY; EMBRYONIC STEM CELL; FLOW CYTOMETRY; GENETICS; LENTIVIRINAE; METABOLISM; NERVE CELL; STEM CELL;

MURINAE;

ANIMALS; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELL LINEAGE; CELL SEPARATION; EMBRYONIC STEM CELLS; FLOW CYTOMETRY; GREEN FLUORESCENT PROTEINS; HUMANS; LENTIVIRUS; MICE; MICRORNAS; NEURONS; STEM CELLS;

EID: 77954923288     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1006568107     Document Type: Article

References (23)

1. Murry CE, Keller G (2008) Differentiation of embryonic stem cells to clinically relevant populations: Lessons from embryonic development. Cell 132:661-680.
2. Chambers SM, et al. (2009) Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling. Nat Biotechnol 27:275-280.
3. Kawasaki H, et al. (2000) Induction of midbrain dopaminergic neurons from ES cells by stromal cell-derived inducing activity. Neuron 28:31-40.
4. Kim JH, et al. (2002) Dopamine neurons derived from embryonic stem cells function in an animal model of Parkinson's disease. Nature 418:50-56.
5. Ying QL, Stavridis M, Griffiths D, Li M, Smith A (2003) Conversion of embryonic stem cells into neuroectodermal precursors in adherent monoculture. Nat Biotechnol 21: 183-186.
6. Aubry L, et al. (2008) Striatal progenitors derived from human ES cells mature into DARPP32 neurons in vitro and in quinolinic acid-lesioned rats. Proc Natl Acad Sci USA 105:16707-16712.
7. Friling S, et al. (2009) Efficient production of mesencephalic dopamine neurons by Lmx1a expression in embryonic stem cells. Proc Natl Acad Sci USA 106:7613-7618.
8. Placantonakis DG, et al. (2009) BAC transgenesis in human embryonic stem cells as a novel tool to define the human neural lineage. Stem Cells 27:521-532.
9. Irion S, et al. (2007) Identification and targeting of the ROSA26 locus in human embryonic stem cells. Nat Biotechnol 25:1477-1482.
10. Bartel DP (2004) MicroRNAs: Genomics, biogenesis, mechanism, and function. Cell 116:281-297.
11. Brown BD, et al. (2007) Endogenous microRNA can be broadly exploited to regulate transgene expression according to tissue, lineage and differentiation state. Nat Biotechnol 25:1457-1467.
12. Brown BD, Venneri MA, Zingale A, Sergi Sergi L, Naldini L (2006) Endogenous microRNA regulation suppresses transgene expression in hematopoietic lineages and enables stable gene transfer. Nat Med 12:585-591.
13. Conti L, et al. (2005) Niche-independent symmetrical self-renewal of a mammalian tissue stem cell. PLoS Biol, 10.1371/journal.pbio.0030283.
14. Marson A, et al. (2008) Connecting microRNA genes to the core transcriptional regulatory circuitry of embryonic stem cells. Cell 134:521-533.
15. Miura K, et al. (2009) Variation in the safety of induced pluripotent stem cell lines. Nat Biotechnol 27:743-745.
16. Takahashi K, et al. (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131:861-872.
17. Takahashi K, Yamanaka S (2006) Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126:663-676.
18. Kaji K, et al. (2009) Virus-free induction of pluripotency and subsequent excision of reprogramming factors. Nature 458:771-775.
19. Lois C, Hong EJ, Pease S, Brown EJ, Baltimore D (2002) Germline transmission and tissue-specific expression of transgenes delivered by lentiviral vectors. Science 295: 868-872.
20. Zufferey R, Nagy D, Mandel RJ, Naldini L, Trono D (1997) Multiply attenuated lentiviral vector achieves efficient gene delivery in vivo. Nat Biotechnol 15:871-875.
21. Georgievska B, et al. (2004) Regulated delivery of glial cell line-derived neurotrophic factor into rat striatum, using a tetracycline- dependent lentiviral vector. Hum Gene Ther 15:934-944.
22. Jönsson ME, Ono Y, Björklund A, Thompson LH (2009) Identification of transplantable dopamine neuron precursors at different stages of midbrain neurogenesis. Exp Neurol 219:341-354.
23. Thompson L, Barraud P, Andersson E, Kirik D, Björklund A (2005) Identification of dopaminergic neurons of nigral and ventral tegmental area subtypes in grafts of fetal ventral mesencephalon based on cell morphology, protein expression, and efferent projections. J Neurosci 25:6467-6477.