Lactococcus lactis as expression host for the biosynthetic incorporation of tryptophan analogues into recombinant proteins

Biochemical Journal

Volumn 409, Issue 1, 2008, Pages 193-198

  El Khattabi, Mohamed ^a,c   Van Roosmalen, Maarten L ^a   Jager, Dennis ^a   Metselaar, Heidi ^a   Permentier, Hjalmar ^a   Leenhouts, Kees ^a   Broos, Jaap ^b  

^a UNIVERSITY OF GRONINGEN   (Netherlands)

^b UNIVERSITY OF GRONINGEN   (Netherlands)

^c UTRECHT UNIVERSITY   (Netherlands)

Author keywords

3 indoleacrilic acid; 5 methyltryptophan; Lactococcus lactis; N phosphonomethylglycine (glyphosate); Tryptophan analogue; Tryptophan auxotroph

Indexed keywords

5-METHYLTRYPTOPHAN; LACTOCOCCUS LACTIS; TRYPTOPHAN ANALOGUE; TRYPTOPHAN AUXOTROPH;

BIOSYNTHESIS; ESCHERICHIA COLI; GENE EXPRESSION; SPECTROSCOPIC ANALYSIS;

PROTEINS;

5 FLUOROTRYPTOPHAN; 5 HYDROXYTRPTOPHAN; 5 METHYLTRYPTOPHAN; 7 AZATRYPTOPHAN; RECOMBINANT PROTEIN; TRYPTOPHAN DERIVATIVE; UNCLASSIFIED DRUG; 5-METHYLTRYPTOPHAN; BACTERIAL PROTEIN; DRUG DERIVATIVE; GLYCINE; GLYPHOSATE; TRYPTOPHAN;

ARTICLE; BIOSYNTHESIS; CULTURE MEDIUM; GRAM POSITIVE BACTERIUM; LACTOCOCCUS LACTIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROKARYOTE; PROTEIN EXPRESSION; SPECTROSCOPY; STRUCTURE ANALYSIS; BIOCHEMISTRY; CHEMISTRY; ESCHERICHIA COLI; GENE DELETION; GENE EXPRESSION REGULATION; MASS SPECTROMETRY; METABOLISM; METHODOLOGY; MOLECULAR CLONING;

ESCHERICHIA COLI; LACTOCOCCUS LACTIS; NEGIBACTERIA; POSIBACTERIA; PROKARYOTA;

BACTERIAL PROTEINS; BIOCHEMISTRY; CLONING, MOLECULAR; ESCHERICHIA COLI; GENE DELETION; GENE EXPRESSION REGULATION, BACTERIAL; GLYCINE; LACTOCOCCUS LACTIS; RECOMBINANT PROTEINS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TRYPTOPHAN;

EID: 38149060175     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20070909     Document Type: Article

References (34)

1. Lakowicz, J. R. (2006) Principles of Fluorescence Spectroscopy, Springer, New York
2. Eftink, M. R. (1991) Fluorescence techniques for studying protein structure. Methods Biochem. Anal. 35, 127-205
3. Ross, J. B., Szabo, A. G. and Hogue, C. W. (1997) Enhancement of protein spectra with tryptophan analogs: fluorescence spectroscopy of protein-protein and protein-nucleic acid interactions. Methods Enzymol. 278, 151-190
4. Broos, J., Gabellieri, E., Biemans-Oldehinkel, E. and Strambini, G. B. (2003) Efficient biosynthetic incorporation of tryptophan and indole analogs in an integral membrane protein. Protein Sci. 12, 1991-2000
5. Arnau, J., Hjerl-Hansen, E. and Israelsen, H. (1997) Heterologous gene expression of bovine plasmin in Lactococcus lactis. Appl. Microbiol. Biotechnol. 48, 331-338
6. Miyoshi, A., Poquet, I., Azevedo, V., Commissaire, J., Bermudez-Humaran, L., Domakova, E., Le Loir, Y., Oliveira, S. C., Gruss, A. and Langella, P. (2002) Controlled production of stable heterologous proteins in Lactococcus lactis. Appl. Environ. Microbiol. 68, 3141-3146
7. Nouaille, S., Ribeiro, L. A., Miyoshi, A., Pontes, D., Le Loir, Y., Oliveira, S. C., Langella, P. and Azevedo, V. (2003) Heterologous protein production and delivery systems for Lactococcus lactis. Genet. Mol. Res. 2, 102-111
8. Kunji, E. R. S., Slotboom, D. J. and Poolman, B. (2003) Lactococcus lactis as host for overproduction of functional membrane proteins. Biochim. Biophys. Acta Biomem. 1610, 97-108
9. de Vos, W. M. (1999) Gene expression systems for lactic acid bacteria. Curr. Opin. Microbiol. 2, 289-295
10. Kuipers, O. P., de Ruyter, P. G. G. A., Kleerebezem, M. and de Vos, W. M. (1997) Controlled overproduction of proteins by lactic acid bacteria. Trends Biotechnol. 15, 135-140
11. Terzaghi, B. E. and Sandine, W. E. (1975) Improved medium for lactic streptococci and their bacteriophages. Appl. Microbiol. 29, 807-813
12. Poolman, B. and Konings, W. N. (1988) Relation of growth of Streptococcus lactis and Streptococcus cremoris to amino-acid transport. J. Bacteriol. 170, 700-707
13. Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989) Molecular Cloning, a Laboratory Manual, Cold Spring Harbor Laboratory Press, Plainview, NY
14. Leenhouts, K., Venema, G. and Kok, J. (1998) A lactococcal pWV01-based integration toolbox for bacteria. Methods Cell Sci. 20, 35-50
15. Holo, H. and Nes, I. F. (1989) High-frequency transformation, by electroporation, of Lactococcus lactis subsp. cremoris grown with glycine in osmotically stabilized media. Appl. Environ. Microbiol. 55, 3119-3123
16. Buist, G., Kok, J., Leenhouts, K. J., Dabrowska, M., Venema, G. and Haandrikman, A. J. (1995) Molecular cloning and nucleotide sequence of the gene encoding the major peptidoglycan hydrolase of Lactococcus lactis, a muramidase needed for cell separation. J. Bacteriol. 177, 1554-1563
17. Steen, A., Buist, G., Leenhouts, K. J., El Khattabi, M., Grijpstra, F., Zomer, A. L., Venema, G., Kuipers, O. P. and Kok, J. (2003) Cell wall attachment of a widely distributed peptidoglycan binding domain is hindered by cell wall constituents. J. Biol. Chem. 278, 23874-23881
18. 19F-labeled Trp side chains for NMR-spectroscopy- based ligand-protein interaction studies. ChemBioChem 4, 649-650
19. Steinrucken, H. C. and Amrhein, N. (1980) The herbicide glyphosate is a potent inhibitor of 5-enolpyruvyl-shikimic-acid 3-phosphate synthase. Biochem. Biophys. Res. Commun. 94, 1207-1212
20. Miles, E. W. (2001) Tryptophan synthase: a multienzyme complex with an intramolecular tunnel. Chem. Rec. 1, 140-151
21. Bardowski, J., Ehrlich, S. D. and Chopin, A. (1992) Tryptophan biosynthesis genes in Lactococcus lactis subsp. lactis. J. Bacteriol. 174, 6563-6570
22. Bolotin, A., Wincker, P., Mauger, S., Jaillon, O., Malarme, K., Weissenbach, J., Ehrlich, S. D. and Sorokin, A. (2001) The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403. Genome Res. 11, 731-753
23. Wegmann, U., O'Connell-Motherwy, M., Zomer, A., Buist, G., Shearman, C., Canchaya, C., Ventura, M., Goesmann, A., Gasson, M. J., Kuipers, O. P. et al. (2007) Complete genome sequence of the prototype lactic acid bacterium Lactococcus lactis subsp cremoris MG1363. J. Bacteriol. 189, 3256-3270
24. Budisa, N., Pal, P. P., Alefelder, S., Birle, P., Krywcun, T., Rubini, M., Wenger, W., Bae, J. H. and Steiner, T. (2004) Probing the role of tryptophans in Aequorea victoria green fluorescent proteins with an expanded genetic code. Biol. Chem. 385, 191-202
25. Ross, J. B. A., Rusinova, E., Luck, L. A. and Rousslang, K. W. (2000) Spectral enhancement of proteins by in vivo incorporation of tryptophan analogues. In Topics in Fluorescence Spectroscopy, Vol. 6: Protein Fluorescence (Lakowicz, J. R., ed.), pp. 17-42, Kluwer Academic/Plenum Publishers, New York
26. +-ATPase of Escherichia coli and NMR-studies. FEBS Lett. 272, 34-36
27. - (555-7). J. Bacteriol. 97, 980-982
28. Ezekiel, D. H., Carlson, N. and Jenkins, T. (1973) Incorporation of 5-methyltryptophan into protein of Escherichia coli. Biochim. Biophys. Acta 312, 751-764
29. Pardee, A. B., Shore, V. G. and Prestridge, L. S. (1956) Incorporation of azatryptophan into proteins of bacteria and bacteriophage. Biochim. Biophys. Acta 21, 406-407
30. Liu, T. Q., Callis, P. R., Hesp, B. H., de Groot, M., Buma, W. J. and Broos, J. (2005) Ionization potentials of fluoroindoles and the origin of nonexponential tryptophan fluorescence decay in proteins. J. Am. Chem. Soc. 127, 4104-4113
31. b transitions and comparison with theory. J. Phys. Chem. 94, 3469-3479
32. Broos, J., Tveen-Jensen, K., de Waal, E., Hesp, B. H., Jackson, J. B., Canters, G. W. and Callis, P. R. (2007) The emitting state of tryptophan in proteins with highly blue-shifted fluorescence. Angew. Chem. Int. Ed. 46, 5137-5139
33. Gasson, M. J. (1983) Plasmid complements of Streptococcus lactis NCDO-712 and other lactic streptococci after protoplast-induced curing. J. Bacteriol. 154, 1-9
34. Bosma, T., Kanninga, R., Neef, J., Audouy, S. A. L., van Roosmalen, M. L., Steen, A., Buist, G., Kok, J., Kuipers, O. P., Robillard, G. and Leenhouts, K. (2006) Novel surface display system for proteins on non-genetically modified Gram-positive bacteria. Appl. Environ. Microbiol. 72, 880-889