Characterization of bacterial artificial chromosome transgenic mice expressing mCherry fluorescent protein substituted for the murine smooth muscle α-actin gene

Genesis

Volumn 48, Issue 7, 2010, Pages 457-463

  Armstrong, John J ^a,b   Larina, Irina V ^a   Dickinson, Mary E ^a   Zimmer, Warren E ^c   Hirschi, Karen K ^a,b  

^a BAYLOR COLLEGE OF MEDICINE   (United States)

^b CENTER FOR CELL AND GENE THERAPY   (United States)

^c TEXAS A AND M HEALTH SCIENCE CENTER   (United States)

Author keywords

BAC transgenesis; Cardiac function; In vivo imaging; Mesenchymal cell; Vascular development

Indexed keywords

ALPHA SMOOTH MUSCLE ACTIN; BIOLOGICAL MARKER; CHERRY RED FLUORESCENT PROTEIN; MEMBRANE PROTEIN; UNCLASSIFIED DRUG; ACTIN; GREEN FLUORESCENT PROTEIN; PHOTOPROTEIN; RED FLUORESCENT PROTEIN;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BACTERIAL ARTIFICIAL CHROMOSOME; CELL ISOLATION; CONTROLLED STUDY; EMBRYO; EMBRYONAL TISSUE; FEMALE; FLUORESCENCE; FLUORESCENCE ACTIVATED CELL SORTING; GENE SEQUENCE; GENE TECHNOLOGY; MALE; MEMBRANE; MOUSE; MUSCLE CELL; NONHUMAN; PERICYTE; POSTNATAL DEVELOPMENT; PRIORITY JOURNAL; PROGENY; PROMOTER REGION; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; SMOOTH MUSCLE FIBER; TRANSGENIC MOUSE; VASCULAR SMOOTH MUSCLE; AGE; ANIMAL; ANIMAL EMBRYO; CELL MEMBRANE; CYTOLOGY; EMBRYOLOGY; FLOW CYTOMETRY; GENETICS; HEART; METABOLISM; PHYSIOLOGY; SPECTROFLUOROMETRY;

BACTERIA (MICROORGANISMS); MURINAE; MUS; MUS MUSCULUS;

ACTINS; AGE FACTORS; ANIMALS; CELL MEMBRANE; CHROMOSOMES, ARTIFICIAL, BACTERIAL; EMBRYO, MAMMALIAN; FLOW CYTOMETRY; GREEN FLUORESCENT PROTEINS; HEART; LUMINESCENT PROTEINS; MICE; MICE, TRANSGENIC; MUSCLE, SMOOTH, VASCULAR; PERICYTES; PROMOTER REGIONS, GENETIC; SPECTROMETRY, FLUORESCENCE;

EID: 77954750336     PISSN: 1526954X     EISSN: 1526968X     Source Type: Journal    
DOI: 10.1002/dvg.20638     Document Type: Article

References (11)

1. Black FA, Packer SE, Parker TG, Lloyd MH, Roberts R, Schwartz RJ, Schneider MD. 1991. The vascular smooth muscle a-actin gene is reactivated during cardiac hypertrophy provoked by load. J Clin Invest 88:1581-1588.
2. Chandler KJ, Chandler RL, Broeckelmann EM, Hou Y, Southard-Smith M, Mortlock DP. 2007. Relevance of BAC transgene copy number in mice: Transgene copy number variation across multiple transgenic lines and correlations with transgene integrity and expression. Mamm Genome 18:693-708.
3. Dyer MA, Farrington SM, Mohn D, Munday JR, Baron MH. 2001. Indian hedgehog activates hematopoiesis and vasculogenesis and can respecify prospective neurectodermal cell fate in the mouse embryo. Development 128:1717-1730.
4. Jones EAV, CrottyD,Kulesa PM, Waters CW, BaronMH, Fraser SE, Dickinson ME. 2002. Dynamic in vivo imaging of postimplantation mammalian embryos using whole embryo culture. Genesis 34:228-235.
5. Lee C, Daiguan Y, Martinez de Velasco J, Tessarollo L, Swing DA, Court DL, Jenkins NA, Copeland NG. 2001. A highly efficient Escherichia coli-based chromosome engineering system adapted for recombinogenic targeting and subcloning of BAC DNA. Genomics 73:56-65.
6. Mack CP, Owens GK. 1999. Regulation of smooth muscle a-actin expression in vivo is dependent on CArG elements within the 50 and first intron promoter regions. Circ Res 84:852-861.
7. Maye P, Stover ML, Liu Y, Rowe DW, Gong S, Lichtler AC. 2009. A BAC-bacterial recombination method to generate physically linked multiple gene reporter DNA constructs. BMC Biotechnol 9:20.
8. McHugh KM. 1995. Molecular analysis of smooth muscle development in the mouse. Dev Dyn 204:278-290.
9. Poser I, Sarov M, Hutchins JRA, Heriche JK, Toyoda Y, Pozniakovsky A, Weigl D, Nitzsche A, Hegemann B, Bird AW, Pelletier A, Kittler R, Hua S, Naumann R, Augsburg M, Sykora MM, Hofemeister H, Zhang Y, Nasmyth K, White KP, Dietzel S, Mechtler K, Durbin R, Stewart AF, Peters JM, Buchholz F, Hyman AA. 2008. BAC TransgeneOmics: A high-throughput method for exploration of protein function in mammals. Nat Methods 5:409-415.
10. Schlidmeyer LA, Braun R, Taffet G, Debiasi M, Burns AE, Bradley A, Schwartz RJ. 2000. Impaired vascular contractility and blood pressure homeostasis in the smooth muscle a-actin null mouse. FASEB J 14:2213-2220.
11. Shaner NC, Campbell RE, Steinbach PA, Giepmans BNG, Palmer AE, Tsien RY. 2004. Improved monomeric red, orange and yellow fluorescent proteins derived from Disconsoma sp. red fluorescent protein. Nat Biotechnol 12:1567-1572.