Characterization of the glycosylation occupancy and the active site in the follow-on protein therapeutic: TNK-tissue plasminogen activator

Analytical Chemistry

Volumn 82, Issue 14, 2010, Pages 6154-6162

  Jiang, Haitao ^a   Wu, Shiaw Lin ^a   Karger, Barry L ^a   Hancock, William S ^a  

^a NORTHEASTERN UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVE SITE; BIOLOGICAL ACTIVITIES; CLEAVAGE SITES; DEAMIDATION; DISULFIDE LINKAGES; DRUG EFFICACY; GLYCOSYLATED; INTACT PROTEINS; N-LINKED; N-TERMINALS; O-LINKED; PRIMARY SEQUENCES; TISSUE PLASMINOGEN ACTIVATOR; TRYPTIC PEPTIDES;

ESTERIFICATION; GLYCOSYLATION; INNOVATION; OXIDATION; PEPTIDES;

DRUG PRODUCTS;

GLYCOPEPTIDE; TISSUE PLASMINOGEN ACTIVATOR; TNK TISSUE PLASMINOGEN ACTIVATOR; TNK-TISSUE PLASMINOGEN ACTIVATOR; TRYPSIN;

AMINO ACID SUBSTITUTION; ARTICLE; CHEMISTRY; ENZYME ACTIVE SITE; GENETICS; GLYCOSYLATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; METABOLISM; METHODOLOGY; POLYACRYLAMIDE GEL ELECTROPHORESIS; SITE DIRECTED MUTAGENESIS; PROCEDURES;

AMINO ACID SUBSTITUTION; CATALYTIC DOMAIN; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GLYCOPEPTIDES; GLYCOSYLATION; MUTAGENESIS, SITE-DIRECTED; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TISSUE PLASMINOGEN ACTIVATOR; TRYPSIN;

EID: 77954653769     PISSN: 00032700     EISSN: None     Source Type: Journal    
DOI: 10.1021/ac100956x     Document Type: Article

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