RGD-dependent binding of TP508 to integrin αvβ 3 mediates cell adhesion and induction of nitric oxide

Thrombosis and Haemostasis

Volumn 104, Issue 1, 2010, Pages 172-182

  Derkach, Dmitry N ^a   Wadekar, Subhagya A ^a   Perkins, Kim B ^a   Rousseau, Emma ^a   Dreiza, Catherine M ^a,b   Cheung Flynn, Joyce ^a   Ramos, Heidi C ^a   Ugarova, Tatiana P ^b   Sheller, Michael R ^a  

^a Capstone Therapeutics   (United States)

^b ARIZONA STATE UNIVERSITY   (United States)

Author keywords

Endothelial dysfunction; eNOS; Integrin; Nitric oxide; TP508

Indexed keywords

ARGINYLGLYCYLASPARTIC ACID; CARBACHOL; FIBRINOGEN; FOCAL ADHESION KINASE; MEMBRANE RECEPTOR; MITOGEN ACTIVATED PROTEIN KINASE 1; MITOGEN ACTIVATED PROTEIN KINASE 3; MONOCLONAL ANTIBODY LM 609; NITRIC OXIDE; PROTHROMBIN; SYNTHETIC PEPTIDE; TP508; UNCLASSIFIED DRUG; VITRONECTIN RECEPTOR;

ARTICLE; CELL ADHESION; CONTROLLED STUDY; DRUG PROTEIN BINDING; ENDOTHELIAL DYSFUNCTION; ENDOTHELIUM CELL; ENZYME PHOSPHORYLATION; HEART MUSCLE ISCHEMIA; HUMAN; HUMAN CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN FAMILY; RAT; REPERFUSION INJURY; SURFACE PLASMON RESONANCE; SYNTHESIS; VASODILATATION;

ANIMALS; ANTIBODIES, BLOCKING; AORTA; CARBACHOL; CELL ADHESION; CELL LINE; ENDOTHELIAL CELLS; FOCAL ADHESION KINASE 2; HUMANS; INTEGRIN ALPHAVBETA3; MALE; MITOGEN-ACTIVATED PROTEIN KINASE 3; NITRIC OXIDE; OLIGOPEPTIDES; PEPTIDE FRAGMENTS; PROTEIN BINDING; RATS; RATS, SPRAGUE-DAWLEY; THROMBIN; VASODILATION;

EID: 77954381261     PISSN: 03406245     EISSN: None     Source Type: Journal    
DOI: 10.1160/TH09-07-0447     Document Type: Article

References (46)

1. Lerman A, Burnett JC, Jr. Intact and altered endothelium in regulation of vasomotion. Circulation 1992; 86: III12-19.
2. Cai H, Harrison DG. Endothelial dysfunction in cardiovascular diseases: the role of oxidant stress. Circulation research 2000; 87: 840-844.
3. Hadi HA, Carr CS, Al Suwaidi J. Endothelial dysfunction: cardiovascular risk factors, therapy, and outcome. Vasc Health Risk Manag 2005; 1: 183-198.
4. Suzuki M, Takamisawa I, Yoshimasa Y, et al. Association between insulin resistance and endothelial dysfunction in type 2 diabetes and the effects of pioglitazone. Diabetes research and clinical practice 2007; 76: 12-17. (Pubitemid 46240368)
5. Rikitake Y, Kim HH, Huang Z, et al. Inhibition of Rho kinase (ROCK) leads to increased cerebral blood flow and stroke protection. Stroke 2005; 36: 2251-2257. (Pubitemid 41429255)
6. Lloyd-Jones DM, Bloch KD. The vascular biology of nitric oxide and its role in atherogenesis. Annu Rev Med 1996; 47: 365-375. (Pubitemid 26138897)
7. Egashira K. Clinical importance of endothelial function in arteriosclerosis and ischemic heart disease. Circ J 2002; 66: 529-533. (Pubitemid 34733489)
8. Katz SD, Hryniewicz K, Hriljac I, et al. Vascular endothelial dysfunction and mortality risk in patients with chronic heart failure. Circulation 2005; 111: 310-314. (Pubitemid 40165337)
9. Boulanger CM. Secondary endothelial dysfunction: hypertension and heart failure. J Mol Cell Cardiol 1999; 31: 39-49. (Pubitemid 29078217)
10. Simonsen U, Rodriguez-Rodriguez R, Dalsgaard T, et al. Novel approaches to improving endothelium-dependent nitric oxide-mediated vasodilatation. Pharmacol Rep 2009; 61: 105-115.
11. Fossum T, Olszewska-Pazdrak B, Mertens M, et al. TP508 (Chrysalin) reverses endothelial dysfunction and increases perfusion and myocardial function in hearts with chronic ischemia J Cardiovasc Pharmacol Ther 2008; 13: 214-225.
12. Osipov R, Bianchi C, Clements R, et al. Thrombin fragment (TP508) decreases myocardial infarction and apoptosis after ischemia reperfusion injury. Ann Thorac Surg 2009; 87: 786-793.
13. Osipov R, Robich M, Feng J, et al. The effect of thrombin fragment (TP508) on myocardial ischemia reperfusion injury in hypercholesterolemic pigs. J Appl Physiol 2009; 106: 1993-2001.
14. Glenn K, Frost G, Bergmann J, et al. Synthetic peptides bind to high-affinity thrombin receptors and modulate thrombin mitogenesis. Pept Res 1988; 1: 65-73.
15. Bar-Shavit R, Sabbah V, Lampugnani MG, et al. An Arg-Gly-Asp sequence within thrombin promotes endothelial cell adhesion. J Cell Biol 1991; 112: 335-344. (Pubitemid 21909623)
16. Papaconstantinou M, Carrell C, Pineda A, et al. Thrombin functions through its RGD sequence in a non-canonical conformation. J Biol Chem 2005; 280: 29393-29396. (Pubitemid 41177010)
17. Bar-Shavit R, Eskohjido Y, Fenton JW, 2nd, et al. Thrombin adhesive properties: induction by plasmin and heparan sulfate. J Cell Biol 1993; 123: 1279-1287. (Pubitemid 23352435)
18. Tsopanoglou N, Papaconstantinou M, Flordellis C, et al. On the mode of action of thrombin-induced angiogenesis: thrombin peptide, TP508, mediates effects in endothelial cells via alphavbeta3 integrin. Thromb Haemost 2004; 92: 846-857.
19. Tsopanoglou NE, Andriopoulou P, Maragoudakis ME. On the mechanism of thrombin-induced angiogenesis: involvement of alphavbeta3-integrin. American journal of physiology 2002; 283: C1501-1510.
20. Sajid M, Zhao R, Pathak A, et al. Alphavbeta3-integrin antagonists inhibit thrombin-induced proliferation and focal adhesion formation in smooth muscle cells. American journal of physiology 2003; 285: C1330-1338.
21. Stouffer GA, Smyth SS. Effects of thrombin on interactions between beta3-integrins and extracellular matrix in platelets and vascular cells. Arterioscler Thromb Vasc Biol 2003; 23: 1971-1978.
22. Yakubenko VP, Lishko VK, Lam SC, et al. A molecular basis for integrin alphaMbeta 2 ligand binding promiscuity. J Biol Chem 2002; 277: 48635-48642.
23. Yakubenko VP, Yadav SP, Ugarova TP. Integrin alphaDbeta2, an adhesion receptor up-regulated on macrophage foam cells, exhibits multiligand-binding properties. Blood 2006; 107: 1643-1650.
24. Bhattacharya S, Fu C, Bhattacharya J, et al. Soluble ligands of the alpha v beta 3 integrin mediate enhanced tyrosine phosphorylation of multiple proteins in adherent bovine pulmonary artery endothelial cells. J Biol Chem 1995; 270: 16781-16787.
25. Boyle JG, Logan PJ, Ewart MA, et al. Rosiglitazone stimulates nitric oxide synthesis in human aortic endothelial cells via AMP-activated protein kinase. J Biol Chem 2008; 283: 11210-11217.
26. Komalavilas P, Penn RB, Flynn CR, et al. The small heat shock-related protein, HSP20, is a cAMP-dependent protein kinase substrate that is involved in airway smooth muscle relaxation. Am J Physiol Lung Cell Mol Physiol 2008; 294: L69-78.
27. Bodary SC, McLean JW. The integrin beta 1 subunit associates with the vitronectin receptor alpha v subunit to form a novel vitronectin receptor in a human embryonic kidney cell line. J Biol Chem 1990; 265: 5938-5941.
28. Giancotti FG, Ruoslahti E. Integrin signaling. Science 1999; 285: 1028-1032.
29. Fossum TW, Olszewska-Pazdrak B, Mertens MM, et al. TP508 (Chrysalin(R)) Reverses Endothelial Dysfunction and Increases Perfusion and Myocardial Function in Hearts With Chronic Ischemia. J Cardiovasc Pharmacol Therap 2008; 13: 214-225.
30. Urbinati C, Mitola S, Tanghetti E, et al. Integrin alphavbeta3 as a target for blocking HIV-1 Tat-induced endothelial cell activation in vitro and angiogenesis in vivo. Arterioscler Thromb Vasc Biol 2005; 25: 2315-2320.
31. Suehiro K, Mizuguchi J, Nishiyama K, et al. Fibrinogen binds to integrin alpha(5)beta(1) via the carboxyl-terminal RGD site of the Aalpha-chain. J Biochem 2000; 128: 705-710.
32. Chen Q, Kinch MS, Lin TH, et al. Integrin-mediated cell adhesion activates mitogen- activated protein kinases. J Biol Chem 1994; 269: 26602-26605. (Pubitemid 24332843)
33. Morino N, Mimura T, Hamasaki K, et al. Matrix/integrin interaction activates the mitogen-activated protein kinase, p44erk-1 and p42erk-2. J Biol Chem 1995; 270: 269-273.
34. Naldini A, Carraro F, Baldari C, et al. The thrombin peptide, TP508, enhances cytokine release and activates signaling events. Peptides 2004; 25: 1917-1926. (Pubitemid 39389186)
35. Koshida R, Rocic P, Saito S, et al. Role of focal adhesion kinase in flow-induced dilation of coronary arterioles. Arterioscler Thromb Vasc Biol 2005; 25: 2548-2553. (Pubitemid 43732277)
36. Katsumi A, Orr AW, Tzima E, et al. Integrins in mechanotransduction. J Biol Chem 2004; 279: 12001-12004.
37. Mineo C, Yuhanna IS, Quon MJ, et al. High density lipoprotein-induced endothelial nitric-oxide synthase activation is mediated by Akt and MAP kinases. J Biol Chem 2003; 278: 9142-9149. (Pubitemid 36800395)
38. Urano T, Ito Y, Akao M, et al. Angiopoietin-related growth factor enhances blood flow via activation of the ERK1/2-eNOS-NO pathway in a mouse hind-limb ischemia model. Arterioscler Thromb Vasc Biol 2008; 28: 827-834.
39. Lovren F, Pan Y, Shukla PC, et al. Visfatin (nicotinomide phosphoribosyltransferase/ pre-B cell colony-enhancing factor) activates eNOS via Akt and MAP kinases and improves endothelial function. Am J Physiol Endocrinol Metab 2009; 296: E1440-1449.
40. Vu T, Hung D, Wheaton V, et al. Molecular cloning of a functional thrombin receptor reveals a novel proteolytic mechanism of receptor activation. Cell 1991; 64: 1057-1068.
41. Ishihara H, Connolly A, Zeng D, et al. Protease-activated receptor 3 is a second thrombin receptor in humans. Nature 1997; 386: 502-506.
42. Kahn ML, Zheng YW, Huang W, et al. A dual thrombin receptor system for platelet activation. Nature 1998; 394: 690-694.
43. Xu WF, Andersen H, Whitmore TE, et al. Cloning and characterization of human protease-activated receptor 4. Proc Natl Acad Sci USA 1998; 95: 6642-6646.
44. Sajid M, Stouffer GA. The role of alpha(v)beta3 integrins in vascular healing. Thromb Haemost 2002; 87: 187-193.
45. Hein TW, Platts SH, Waitkus-Edwards KR, et al. Integrin-binding peptides containing RGD produce coronary arteriolar dilation via cyclooxygenase activation. Am J Physiol Heart Circ Physiol 2001; 281: H2378-2384.
46. Shyy JY, Chien S. Role of integrins in endothelial mechanosensing of shear stress. Circulation Res 2002; 91: 769-775. (Pubitemid 35285073)