Microbiota of Karakačanski skakutanac, an artisanal fresh sheep cheese studied by culture-independent PCR-ARDRA and PCR-DGGE

Dairy Science and Technology

Volumn 90, Issue 4, 2010, Pages 461-468

  Pogačić, Tomislav ^a   Samaržija, Dubravka ^a   Corich, Viviana ^b   D'Andrea, Maura ^b   Kagkli, Dafni Maria ^b   Giacomini, Alessio ^b   Čanzek Majhenič, Andreja ^c   Rogelj, Irena ^c  

^a UNIVERSITY OF ZAGREB   (Croatia)

^b UNIVERSITY OF PADOVA   (Italy)

^c UNIVERSITY OF LJUBLJANA   (Slovenia)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA (MICROORGANISMS); FRIESIA; LACTOCOCCUS LACTIS; OVIS ARIES;

EID: 77954356394     PISSN: 19585586     EISSN: 19585594     Source Type: Journal    
DOI: 10.1051/dst/2010004     Document Type: Conference Paper

References (19)

1. Bonetta S., Bonetta S., Carrraro E., Rantsiou K., Cocolin L., Microbiological characterization of Robiola di Roccaverano cheese using PCR-DGGE, Food Microbiol. 25 (2008) 786-792.
2. Cocolin L., Manzano M., Cantoni C., Comi G., Denaturing gradient gel electrophoresis analysis of the 16S rRNA genes V1 region to monitor dynamic changes in the bacterial population during fermentation of Italian sausages, Appl. Environ. Microbiol. 67 (2001) 5113-5121.
3. Duthoit F., Callon C., Tessier L., Montel M.-C., Relationship between sensorial characteristics and microbial dynamics in registered designation of origin Salers cheese, Int. J. Food Microbiol. 103 (2005) 259-270.
4. Duthoit F., Godon J.-J., Montel M.-C., Bacterial community dynamics during production of registered designation of origin Salers cheese as evaluated by 16S rRNA gene single-strand conformation polymorphism analysis, Appl. Environ. Microbiol. 69 (2003) 3840-3848.
5. Ercolini D., PCR-DGGE fingerprinting: novel strategies for detection of microbes in food, J. Microbiol. Methods 56 (2004) 297-314.
6. Ercolini D., Hill P.J., Dodd C.E.R., Bacterial community structure and location in Stilton cheese, Appl. Environ. Microbiol. 69 (2003) 3540-3548.
7. Ercollini D., Moschetti G., Blaiotta G., Copola S., Behaviour of variable V3 region from 16S rDNA of lactic acid bacteria in denaturing gradient gel electrophoresis, Curr. Microbiol. 42 (2001) 199-202.
8. Fontana C., Vignolo G., Cocconceli P.S., PCR-DGGE analysis for the identification of microbial populations from Argentinean dry sausages, J. Microbiol. Methods 63 (2005) 254-263.
9. Gich F.B., Amer E., Figueras J.B., Abella C.A., Balaguer D.M., Poch M., Assessment of microbial community structure changes by amplified ribosomal DNA restriction analysis (ARDRA), Int. Microbiol. 3 (2000) 103-106.
10. Guthrie J., Moriarty D., Blackall L., DNA extraction from coral reef sediment for the PCR, J. Microbiol. Methods 43 (2000) 73-80.
11. Marino M., Maifreni M., Rondini G., Microbiological characterization of artisanal Montasio cheese, FEMS Microbiol. Lett. 229 (2003) 133-140.
12. Marzorati M., Wittebolle L., Boon N., Daffonchio D., Verstraete W., How to get more out of molecular fingerprints: practical tools for microbial ecology, Environ. Microbiol. 10 (2008) 1571-1581.
13. Muyzer G., De Wall E.C., Uitterlinden A.G., Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA, Appl. Environ. Microbiol. 59 (1993) 695-700.
14. Randazzo C.L., Caggia C., Neviani E., Application of molecular approaches to study lactic acid bacteria in artisanal cheeses, J. Microbiol. Methods 78 (2009) 1-9.
15. Randazzo C.L., Torriani S., Akkermans A.D.L., De Vos W.M., Vaughan E.E., Diversity, dynamics and activity of bacterial communities during production of an artisanal Sicillian cheese as evaluated by 16S rRNA analysis, Appl. Environ. Microbiol. 68 (2002) 1882-1892.
16. Rantsiou K., Urso R., Dolci P., Comi G., Cocolin L., Microflora of Feta cheese from Greek manufacturers, Int. J. Food Microbiol. 126 (2008) 36-42.
17. Rodas A., Ferrer Sergi M., Isabel P., 16SARDRA a tool for identification of lactic acid bacteria isolated from grape must and wine, Syst. Appl. Microbiol. 26 (2003) 412-422.
18. Sanguinetti C.J., Dias Neto E., Simpson A.J., Rapid silver staining and recovery of PCR products separated on polyacrylamide gels, Biotechniques 17 (1994) 914-921.
19. Urlik E., Rogall T., Blocker H., Emde M., Bottger E.C., Isolation and direct complete nucleotide determination of entire genes. Characterization of a gene coding for 16S ribosomal RNA, Nucl. Acid Res. 17 (1989) 7843-7853.