Recovery of nicotine-free proteins from tobacco leaves using phosphate buffer system under controlled conditions

Bioresource Technology

Volumn 101, Issue 6, 2010, Pages 2034-2042

  Fu, H ^a,b   Machado, P A ^a   Hahm, T S ^a   Kratochvil, R J ^a   Wei, C I ^a   Lo, Y M ^a  

^a UNIVERSITY OF MARYLAND   (United States)

^b FUZHOU UNIVERSITY   (China)

Author keywords

Buffer; Nicotine; Phosphoric acid; Protein; Value added

Indexed keywords

ACETONE; NICOTINE; PHOSPHORIC ACID; PLANTS (BOTANY); RECOVERY; TOBACCO;

BUFFER; BUFFER CONCENTRATIONS; CONTROLLED CONDITIONS; INDUSTRIAL PRODUCTION; OPTIMAL PROCESSING; PHOSPHATE BUFFERS; PROCESSING SYSTEMS; VALUE-ADDED;

PROTEINS;

ACETONE; ALKALOID; CHLOROPHYLL; NICOTINE; PHOSPHATE BUFFERED SALINE; PHOSPHORIC ACID; BUFFER; PHOSPHATE; VEGETABLE PROTEIN;

ALKALOID; BUFFERING; EXTRACTION METHOD; LEAF; PHOSPHATE; PHYTOCHEMISTRY; PROTEIN; TOBACCO;

ARTICLE; HIGH THROUGHPUT SCREENING; INDUSTRIAL PRODUCTION; NONHUMAN; PH; PLANT LEAF; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN STABILITY; RESPONSE SURFACE METHOD; SCALE UP; STORAGE TEMPERATURE; TEMPERATURE; TOBACCO; TOBACCO INDUSTRY; ULTRAFILTRATION; AGRICULTURE; BIOTECHNOLOGY; CHEMICAL ANALYSIS; CHEMISTRY; METABOLISM; METHODOLOGY;

NICOTIANA TABACUM;

ACETONE; AGRICULTURE; BIOTECHNOLOGY; BUFFERS; CHEMISTRY TECHNIQUES, ANALYTICAL; HYDROGEN-ION CONCENTRATION; NICOTINE; PHOSPHATES; PHOSPHORIC ACIDS; PLANT LEAVES; PLANT PROTEINS; TEMPERATURE; TOBACCO;

EID: 77954310160     PISSN: 09608524     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.biortech.2009.10.045     Document Type: Article

References (46)

1. Altman, D.G., Zaccaro, D.J., Levine, D.W., Austin, D., Woodell, C., Bailey, B., Sligh, M., Cohn, G., Dunn, J., 1998. Predictors of crop diversification: a survey of tobacco farmers in North Carolina (USA). Tob. Control 7 (4), 376-382.
2. Balasubramaniam, D., Wilkinson, C., Van Cott, K., Zhang, C., 2003. Tobacco protein separation by aqueous two-phase extraction. J. Chromatogr. A 989 (1), 119-129.
3. Bonner, P.L.R., 2007. Groundwork. In: Bonner, P.L.R. (Ed.), Protein Purification. Taylor & Francis, New York, p. 26.
4. Bradford, M.M., 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal. Biochem. 72, 248-254.
5. Cramer, C.L., Weissenborn, D.L., Oishi, K.K., Grabau, E.A., Bennett, S., Ponce, E., Grabowski, G.A., Radin, D.N., 1996. Bioproduction of human enzymes in transgenic tobacco. Ann. NY Acad. Sci. 792, 62-71.
6. Dash, A.K., Wong, S.T., 1996. Liquid chromatographic method for the determination of nicotine in pharmaceutical formulations. J. Chromatogr. A 749, 81-85.
7. Degl'Innocenti, E., Guidi, L., Pardossi, A., Tognoni, F., 2005. Biochemical study of leaf browning in minimally processed leaves of lettuce (Lactuca sativa L.Var. Acephala). J. Agr. Food Chem. 53, 9980-9984.
8. Fantozzi, P., Sensidoni, A., 1983. Protein extraction from tobacco leaves: technological, nutritional and agronomical aspects. In: Bodwell, C.E., Petit, L. (Eds.), Plant Protein for Human Food. W. Junk Publishers, New York, pp. 147-149.
9. Fecker, L.F., Rugenhagen, C., Berlin, J., 1993. Increased production of cadaverine and anabasine in hairy root cultures of Nicotiana tabacum expressing a bacterial lysine decarboxylase gene. Plant Mol. Biol. 23, 11-21.
10. Federal Register, 2005. Tobacco transition payment program; final rule. Commodity Credit Corporation and Farm Service Agency, USDA. Federal Register 70 (63), 17150-17166..
11. Fennema, O.R., 1996. Water and ice. In: Fennema, O.R. (Ed.), Food Chemistry. Marcel Dekker, Inc., New York, pp. 32-33.
12. Haschemeyer, R.H., 1973. Fractionation and analysis of proteins. In: Haschemeyer, R.H. (Ed.), Proteins: A Guide to Study by Physical and Chemical Methods. John Wiley & Sons Inc., New York, p. 35.
13. Hatti-Kaul, R., Mattiasson, B., 2003. Release of protein from biological host. In: Hatti-Kaul, R., Mattiasson, B. (Eds.), Isolation and Purification of Proteins. Marcel Dekker, Inc., New York, p. 1.
14. Henningfield, J.E., Benowitz, N.L., Slade, J., Houston, T.P., 1998. Reducing the addictiveness of cigarettes. Tob. Control 7, 281-293.
15. Holmstedt, B., 1988. Toxicity of nicotine and related compounds. In: Rand, M.J., Thurau, K. (Eds.), The Pharmacology of Nicotine. IRL Press, Mclean, pp. 61-68.
16. Huisman, I.H., Prádanos, P., Hernández, A., 2000. The effect of protein-protein and protein-membrane interactions on membrane fouling in ultrafiltration. J. Membr. Sci. 179, 79-90.
17. ++. Photosynth. Res. 87, 195-204.
18. Komatsu, S., Masuda, T., Hirano, H., 1996. Rice gibberellin-binding phosphoprotein structurally related to ribulose-1,5-bisphosphate carboxylase/oxygenase activase. FEBS Lett. 384, 167-171.
19. Kruger, N.J., 1994. The Bradford method for protein quantitation. In: Walker, J.M. (Ed.), Basic Protein and Peptide Protocols. Humana Press Inc., Totowa, pp. 9-15.
20. Kurucu, S., Kartal, M., Erenmemisoglu, A., 1998. HPLC analysis of Nicotiana restica L. and chewing tobacco alkaloids. Farm. Bilimler Derg. 23, 61-64.
21. Kutchan, T.M., 1995. Alkaloid biosynthesis - the basis for metabolic engineering of medicinal plants. Plant Cell 7, 1059-1070.
22. Leite, A., Kemper, E.L., da Silva, M.J., Luchessi, A.D., Siloto, R.M.P., Bonaccorsi, E.D., El-Dorry, H.F., Arruda, P., 2000. Expression of correctly processed human growth hormone in seeds of transgenic tobacco plants. Mol. Breeding 6 (1), 47-53.
23. Lewis, C.A., York Jr., B.M., 1978. The toxicity of plant alkaloids: an ecogeographic perspective. Biochem. Syst. Ecol. 6, 61-76.
24. Lo, Y.M., Fu, H., 2008. Methods for tetragonal crystallization of ribulose 1.5-bisphosphate carboxylase/oxygenase from photosynthetic plant leaves. PCT/US2008/06205.
25. Ma, J.K.C., Lehner, T., Stabila, P., Fux, C.I., 1994. Assembly of monoclonal antibodies with IgG1 and IgA heavy chain domains in transgenic tobacco plants. Eur. J. Immunol. 24 (1), 131-138.
26. Machado, P.A., 2008. Feasibility of extracting solanesol from tobacco biomass as a byproduct following protein recovery. M.S. Thesis, Department of Nutrition and Food Science, University of Maryland, College Park, MD.
27. Mackay, J., Eriksen, M.P., 2002. The Tobacco Atlas. World Health Organization, Geneva.
28. Mason, H.S., Arntzen, C.J., 1995. Transgenic plants as vaccine production systems. Trends Biotechnol. 13 (9), 388-392.
29. Molina, A., Hervás-Stubbs, S., Daniell, H., Mingo-Castel, A.M., Veramendi, J., 2004. High-yield expression of a viral peptide animal vaccine in transgenic tobacco chloroplasts. Plant Biotechnol. J. 2 (2), 141-153.
30. Nelson, P.E., Surzycki, S.J., 1976. A Mutant strain of Chlamydomonas reinhardi exhibiting altered ribulosebisphosphate carboxylase. Eur. J. Biochem. 61, 465-474.
31. Nordic Council of Ministers, 2003. Nicotine Alkaloids in Solanaceous Food Plants. TemaNord 2003, Ekspressen Tryk & Kopicenter, Copenhagen, p. 531.
32. Novotny, T.E., Zhao, F., 1999. Consumption and production waste: another externality of tobacco use. Tob. Control 8, 75-80.
33. Platis, D., Labrou, N.E., 2006. Development of an aqueous two-phase partitioning system for fractionating therapeutic proteins from tobacco extract. J. Chrommatogr. A 1128, 114-124.
34. Robinson, S.P., Streusand, V.J., Chatfield, J.M., Portis Jr., A.R., 1988. Purification and assay of Rubisco Activase from leaves. Plant Physiol. 88, 1008-1014.
35. Salmon, V., Legrand, D., Slomianny, M.-C., Yazidi, I.E., Spik, G., Gruber, V., Bournat, P., Olagnier, B., Mison, D., Theisen, M., Mérot, B., 1998. Production of human lactoferrin in transgenic tobacco plants. Protein Expres. Purif. 13 (1), 127-135.
36. Salvucci, M., Anderson, J., 1987. Factors affecting the activation state and the level of total activity of Ribulose bisphosphate carboxylase in tobacco protoplasts. Plant Physiol. 85, 66-71.
37. Schouten, A., Roosien, J., Van Engelen, F.A., De Jong, G.A.M., Borst-Vrenssen, A.W.M., Zilverentant, J.F., Bosch, D., Stiekema, W.J., Gommers, F.J., Schots, A., 1996. The C-terminal KDEL sequence increases the expression level of a single-chain antibody designed to be targeted to both the cytosol and the secretory pathway in transgenic tobacco. Plant Mol. Biol. 30 (4), 781-793.
38. Scopes, R.K., 1987. Making an extract. In: Scopes, R.K. (Ed.), Protein Purification Principles and Practice. Springer Verlag, New York, p. 26.
39. Seeman, J.I., 1984. Recent studies in nicotine chemistry: conformational analysis, chemical reactivity studies, and theoretical modeling. Heterocycles 22 (1), 165-193.
40. Siegmund, B., Leitner, E., Pfannhauser, W., 1999. Determination of the nicotine content of various edible nightshades (Solanaceae) and their products and estimation of the associated dietary nicotine intake. J. Agr. Food Chem. 47 (8), 3113-3120.
41. Simpson, R.J., 2004. Preparing proteins for proteomic analysis. In: Simpson, R.J. (Ed.), Purifying Proteins for Proteomics: A Laboratory Manual. Cold Harbor Laboratory Press, New York, p. 17.
42. Staub, J.M., Garcia, B., Graves, J., Hajdukiewicz, P.T., Hunter, P., Nehra, N., Paradkar, V., Schlittler, M., Carroll, J.A., Spatola, L., 2000. High-yield production of a human therapeutic protein in tobacco chloroplasts. Nat. Biotechnol. 18 (3), 333-338.
43. Tso, T.C., 1990. Organic metabolism tobacco proteins. In: Tso, T.C. (Ed.), Production, Physiology, and Biochemistry of Tobacco Plant. Ideals Inc., Beltsville, p. 487.
44. Tso, T.C., Kung, S.D., 1983. Soluble proteins in tobacco and their potential use. In: Telek, L., Graham, H.D. (Eds.), Leaf Protein Concentrates. AVI Publishing Company, Westport, pp. 117-129.
45. US Department of Agriculture, 2008. Statistics of cotton, tobacco, sugar, crops and honey. In: Agriculture Statistics 2008. National Agricultural Statistics Service, USDA, Washington, DC. .
46. Vaquero, C., Sack, M., Chandler, J., Drossard, J., Schuster, F., Monecke, M., Schillberg, S., Fischer, R., 1999. Transient expression of a tumor-specific single-chain fragment and a chimeric antibody in tobacco leaves. Proc. Natl. Acad. Sci. USA 96 (20), 11128-11133.