Metabolic engineering of Gluconobacter oxydans for improved growth rate and growth yield on glucose by elimination of gluconate formation

Applied and Environmental Microbiology

Volumn 76, Issue 13, 2010, Pages 4369-4376

  Krajewski, Vera ^a   Simić, Petra ^b   Mouncey, Nigel J ^c   Bringer, Stephanie ^a   Sahm, Hermann ^a   Bott, Michael ^a  

^a FORSCHUNGSZENTRUM JÜLICH   (Germany)

^b DSM NUTRITIONAL PRODUCTS LTD   (Switzerland)

^c Dow Agro Sciences LLC   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACETIC ACID BACTERIA; END-PRODUCTS; ENTNER-DOUDOROFF; GLUCONOBACTER; GLUCONOBACTER OXYDANS; GLUCOSE 6 PHOSPHATES; GLUCOSE DEHYDROGENASE; GLUCOSE METABOLISM; GROWTH YIELD; INTERMEDIATE FORMATION; KETOGLUCONATE; MEMBRANE-BOUND; METABOLIC ENGINEERING; MUTANT STRAIN; NEGATIVE IMPACTS; PARENTAL STRAINS; PENTOSE PHOSPHATE PATHWAY; PERIPLASM; PRODUCT FORMATION; PYRUVATE DECARBOXYLASE; SECONDARY PRODUCT;

ALDEHYDES; CARBON DIOXIDE; CYTOLOGY; ENCODING (SYMBOLS); GENE ENCODING; METABOLISM; PHOSPHORYLATION;

GLUCOSE;

CARBON DIOXIDE; GLUCONIC ACID; GLUCOSE; GLUCOSE DEHYDROGENASE;

ACETIC ACID; BACTERIUM; CARBON DIOXIDE; CYTOPLASM; ENZYME ACTIVITY; ESTER; GLUCOSE; GROWTH RATE; GROWTH RESPONSE; METABOLISM; MICROBIAL ACTIVITY; OXIC CONDITIONS; OXIDATION;

ARTICLE; BIOTECHNOLOGY; CULTURE MEDIUM; EVALUATION; GENE DELETION; GENE EXPRESSION REGULATION; GENETIC ENGINEERING; GENETICS; GLUCONOBACTER OXYDANS; GROWTH, DEVELOPMENT AND AGING; METABOLISM; METHODOLOGY; MUTATION;

BIOTECHNOLOGY; CARBON DIOXIDE; CULTURE MEDIA; GENE DELETION; GENE EXPRESSION REGULATION, BACTERIAL; GENETIC ENGINEERING; GLUCONATES; GLUCONOBACTER OXYDANS; GLUCOSE; GLUCOSE 1-DEHYDROGENASE; MUTATION;

BACTERIA (MICROORGANISMS); GLUCONOBACTER; GLUCONOBACTER OXYDANS;

EID: 77954289367     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.03022-09     Document Type: Article

References (44)

1. +-dependent acetaldehyde dehydrogenase from Zymomonas mobilis. Isolation and partial characterization. Arch. Microbiol. 153:95-100.
2. Bauchop, T., and E. A. Dawes. 1959. Metabolism of pyruvic and formic acids of Zymosarcina ventriculi. Biochim. Biophys. Acta 36:294-296.
3. Bernt, E., and I. Gutmann. 1974. Aethanol, p. 1545-1548. In H. U. Bergmeyer (ed.), Methoden der enzymatischen Analyse, vol. 2. Verlag Chemie, Weinheim, Germany.
4. Boyer, H. W., and D. Roulland-Dussoix. 1969. A complementation analysis of the restriction and modification of DNA in Escherichia coli. J. Mol. Biol. 41:459-472.
5. Bringer-Meyer, S., K.-L. Schimz, and H. Sahm. 1986. Pyruvate decarboxylase from Zymomonas mobilis. Isolation and partial characterization. Arch. Microbiol. 146:105-110.
6. Choi, K.-H., A. Kumar, and H. P. Schweizer. 2006. A 10-min method for preparation of highly electrocompetent Pseudomonas aeruginosa cells: application for DNA fragment transfer between chromosomes and plasmid transformation. J. Microbiol. Methods 64:391-397. doi:10.1016/j.mimet.2005.06.001.
7. Cohen, S. N., A. C. Y. Chang, and L. Hsu. 1972. Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA. Proc. Natl. Acad. Sci. U. S. A. 69:2110-2114.
8. Dawes, E. A., D. W. Ribbons, and P. J. Large. 1966. The route of ethanol formation in Zymomonas mobilis. Biochem. J. 98:795-803.
9. DeLey, J., and J. Schell. 1962. Lactate and pyruvate catabolism in acetic acid bacteria. J. Gen. Microbiol. 29:589-601.
10. Deppenmeier, U., M. Hoffmeister, and C. Prust. 2002. Biochemistry and biotechnological applications of Gluconobacter strains. Appl. Microbiol. Biotechnol. 60:233-242. doi:10.1007/s00253-002-1114-5.
11. Figurski, D. H., and D. R. Helinski. 1979. Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans. Proc. Natl. Acad. Sci. U. S. A. 76:1648-1652.
12. Finn, R. K., S. Bringer, and H. Sahm. 1984. Fermentation of arabinose to ethanol by Sarcina ventriculi. Appl. Microbiol. Biotechnol. 19:161-166.
13. Gupta, A., V. K. Singh, G. N. Qazi, and A. Kumar. 2001. Gluconobacter oxydans: its biotechnological applications. J. Mol. Microbiol. Biotechnol. 3:445-456.
14. Hanahan, D. 1983. Studies on transformation of Escherichia coli with Plasmids. J. Mol. Biol. 166:557-580.
15. Herrmann, U., M. Merfort, M. Jeude, S. Bringer-Meyer, and H. Sahm. 2004. Biotransformation of glucose to 5-keto-D-gluconic acid by recombinant Gluconobacter oxydans DSM 2343. Appl. Microbiol. Biotechnol. 64:86-90. doi: 10.1007/s00253-003-1455-8.
16. Intorne, A. C., M. Vinicius, V. de Oliveira, M. L. Lima, J. F. da Silva, F. L. Olivares, and G. A. de Souza Filho. 2009. Identifcation and characterization of Gluconacetobacter diazotrophicus mutants defective in the solubilisation of phosphorus and zinc. Arch. Microbiol. 191:477-483. doi:10.1007/s00203-009-0472-0.
17. King, T. E., and V. H. Cheldelin. 1954. Pyruvic carboxylase of Acetobacter suboxydans. J. Biol. Chem. 208:821-831.
18. Klasen, R., S. Bringer-Meyer, and H. Sahm. 1995. Biochemical characterization and sequence analysis of the gluconate:NADP 5-oxidoreductase gene from Gluconobacter oxydans. J. Bacteriol. 177:2637-2643.
19. Kovach, M. E., P. H. Elzer, D. S. Hill, G. T. Robertson, M. A. Farris, R. M. Roop II, and K. M. Peterson. 1995. Four new derivatives of the broad-hostrange cloning vector pBBR1MCS carrying different antibiotic-resistance cassettes. Gene 166:175-176.
20. Kovach, M. E., R. W. Phillips, P. H. Elzer, R. M. Roop II, and K. M. Peterson. 1994. pBBR1MCS: a broad-host-range cloning vector. Biotechniques 16:800-802.
21. Krajewski, V. 2008. Modifikation des Glucosestoffwechsels in Gluconobacter oxydans. Ph.D. thesis. University of Düsseldorf, Düsseldorf, Germany.
22. Lam, S. T., B. S. Lam, and G. Strobel. 1985. A vehicle for the introduction of transposons into plant-associated pseudomonads. Plasmid 13:200-204.
23. Link, A. J., D. Phillips, and G. M. Church. 1997. Methods for generating precise deletions and insertions in the genome of wild-type Escherichia coli: application to open reading frame characterization. J. Bacteriol. 179:6228-6237.
24. Madigan, M. T., J. M. Martinko, and J. Parker. 1997. Brock biology of microorganisms, 8th ed. Prentice Hall, Upper Saddle River, NJ.
25. Matsushita, K., H. Toyama, and O. Adachi. 1994. Respiratory chains and bioenergetics of acetic acid bacteria. Adv. Microbiol. Physiol. 36:247-301.
26. Merfort, M., U. Herrmann, S. Bringer-Meyer, and H. Sahm. 2006. Highyield 5-keto-D-gluconic acid formation is mediated by soluble and membrane-bound gluconate-5-dehydrogenases of Gluconobacter oxydans. Appl. Microbiol. Biotechnol. 73:443-451. doi:10.1007/s00253-006-0467-6. (Pubitemid 350262621)
27. Ng, H. 1969. Effect of decreasing growth temperature on cell yield of Escherichia coli. J. Bacteriol. 98:232-237.
28. 1. Arch. Microbiol. 175:282-294.
29. Olijve, W., and J. J. Kok. 1979. Analysis of growth of Gluconobacter oxydans in glucose containing media. Arch. Microbiol. 121:283-290.
30. Pronk, J. T., P. R. Levering, W. Olijve, and J. P. van Dijken. 1989. Role of NADF-dependent and quinoprotein glucose dehydrogenases in gluconic acid production by Gluconobacter oxydans. Enzyme Microb. Technol. 11: 160-164.
31. Prust, C., M. Homneister, H. Liesegang, A. Wiezer, W. F. Fricke, A. Ehrenreich, G. Gottschalk, and U. Deppenmeier. 2005. Complete genome sequence of the acetic acid bacterium Gluconobacter oxydans. Nat. Biotechnol. 23:195-200. doi:10.1038/nbt1062.
32. Reichstein, T., and A. Grüssner. 1934. Eine ergiebige Synthese der 1-Ascorbinsäure (C-Vitamin). Helvetica Chimica Acta 17:311-328.
33. Sambrook, J., and D. W. Russell. 2000. Molecular cloning: a laboratory manual, 3rd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
34. Schäfer, A., A. Tauch, W. Jäger, J. Kalinowski, G. Thierbach, and A. Pühler. 1994. Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum. Gene 145:69-73. (Pubitemid 24224965)
35. Schedel, M. 2000. Regioselective oxidation of aminosorbitol with Gluconobacter oxydans, key reaction in the industrial 1-deoxynojirimycin synthesis, p. 295-311. In H.-J. Rehm and G. Reed (ed.), Biotechnology II. WileyVCH Verlag GmbH, Weinheim, Germany.
36. Schweiger, P., S. Volland, and U. Deppenmeier. 2007. Overproduction and characterization of two distinct aldehyde-oxidizing enzymes from Gluconobacter oxydans 621H. J. Mol. Microbiol. Biotechnol. 13:147-155.
37. 2 metabolism, p. 479-541. In J. R. Morris and D. W. Ribbons (ed.), Methods in enzymology, vol. 6A. Academic Press, New York, NY.
38. Shen, L. C., and D. E. Atkinson. 1970. Regulation of pyruvate dehydrogenase from Escherichia coli-interactions of adenylate energy charge and other regulatory parameters. J. Biol. Chem. 245:5974-5978.
39. Shinagawa, E., Y. Ano, T. Yakushi, O. Adachi, and K. Matsushita. 2009. Solubilization, purification and properties of membrane-bound D-gluconoδ-lactone hydrolase from Gluconobacter oxydans. Biosci. Biotechnol. Biochem. 73:241-244.
40. Shinjoh, M., Y. Setoguchi, T. Hoshino, and A. Fujiwara. 1990. L-Sorbose dissimilation in 2-keto-L-gulonic acid-producing mutant UV10 derived from Gluconobacter melanogenus IFO 3293. Agric. Biol. Chem. 54:2257-2263.
41. Simon, R., U. Priefer, and A. Pühler. 1983. A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in Gramnegative bacteria. Biotechnology 1:784-791. doi:10.1038/nbt1183-784. (Pubitemid 14239484)
42. Sugisawa, T., T. Hoshino, S. Masuda, S. Nomura, Y. Setoguchi, M. Tazoe, M. Shinjoh, S. Someha, and A. Fujiwara. 1990. Microbial production of 2-ketoL-gulonic acid from L-sorbose and L-sorbitol by Gluconobacter melanogenus. Agric. Biol. Chem. 45:1201-1209.
43. Träger, M., U. Müller, and U. Onken. 1987. Einfluss erhöhter SauerstoffPartialdrucke auf die Bildung von Gluconsäure durch Gluconobacter oxydans. Chem. Ing. Tech. 59:940-944.
44. Vennesland, B. 1951. Keto acid decarboxylases, p. 183-215. In P. D. Boyer, H. Lardy, and K. Myrbick (ed.), The enzymes, vol. 2. Academic Press, New York, NY.