A proximity ligation assay using transiently transfected, epitope-tagged proteins: Application for in situ detection of dimerized receptor tyrosine kinases

BioTechniques

Volumn 48, Issue 2, 2010, Pages 145-152

  Gajadhar, Aaron ^a   Guha, Abhijit ^b  

^a HOSPITAL FOR SICK CHILDREN   (Canada)

^b UNIVERSITY OF TORONTO   (Canada)

Author keywords

Dimerization; Epidermal growth factor receptor; Epitope tagging; In situ; Protein interaction; Proximity ligation assay; Receptor tyrosine kinase

Indexed keywords

EPIDERMAL GROWTH FACTOR RECEPTOR; EPITOPE; FLAG PROTEIN; MYC PROTEIN; PROTEIN; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; CONTROLLED STUDY; DIMERIZATION; FEMALE; GENETIC TRANSFECTION; IN SITU DETECTION; NONHUMAN; PROTEIN ANALYSIS; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN INTERACTION; PROTEIN TARGETING; PROXIMITY LIGATION ASSAY; QUANTITATIVE ANALYSIS; VALIDATION STUDY; ANIMAL; CELL LINE; GENETICS; HUMAN; METABOLISM; METHODOLOGY; PROTEIN MULTIMERIZATION; WESTERN BLOTTING;

ANIMALS; BLOTTING, WESTERN; CELL LINE; EPITOPES; HUMANS; PROTEIN INTERACTION MAPPING; PROTEIN MULTIMERIZATION; RECEPTOR, EPIDERMAL GROWTH FACTOR; TRANSFECTION;

EID: 77954000502     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/000113354     Document Type: Article

References (20)

1. Hynes, N.E. and H.A. Lane. 2005. ERBB receptors and cancer: the complexity of targeted inhibitors. Nature Reviews 5:341-354.
2. Yarden, Y. and J. Schlessinger. 1987. Epidermal growth factor induces rapid, reversible aggregation of the purified epidermal growth factor receptor. Biochemistry 26:1443-1451. (Pubitemid 17045875)
3. Yarden, Y. and J. Schlessinger. 1987. Self-phosphorylation of epidermal growth factor receptor: evidence for a model of intermolecular allosteric activation. Biochemistry 26:1434-1442. (Pubitemid 17045874)
4. Fredriksson, S., M. Gul lberg, J. Jarvius, C. Olsson, K. Pietras, S.M. Gustafsdottir, A. Ostman, and U. Landegren. 2002. Protein detection using proximity-dependent DNA ligation assays. Nat. Biotechnol. 20:473-477.
5. Soderberg, O., M. Gullberg, M. Jarvius, K. Ridderstrale, K.J. Leuchowius, J. Jarvius, K. Wester, P. Hydbring, et al. 2006. Direct observation of individual endogenous protein complexes in situ by proximity ligation. Nat. Methods 3:995-1000.
6. Jarvius, M., J. Paulsson, I. Weibrecht, K.J. Leuchowius, A.C. Andersson, C. Wahlby, M. Gullberg, J. Botling, et al. 2007. In situ detection of phosphorylated platelet-derived growth factor receptor beta using a generalized proximity ligation method. Mol. Cell. Proteomics 6:1500-1509.
7. Downward, J., P. Parker, and M.D. Waterfield. 1984. Autophosphorylation sites on the epidermal growth factor receptor. Nature 311:483-485. (Pubitemid 14035459)
8. Garrett, T.P., N.M. McKern, M. Lou, T.C. Elleman, T.E. Adams, G.O. Lovrecz, H.J. Zhu, F. Walker, et al. 2002. Crystal structure of a truncated epidermal growth factor receptor extracellular domain bound to transforming growth factor alpha. Cell 110:763-773.
9. Walker, F., S.G. Orchard, R.N. Jorissen, N.E. Hall, H.H. Zhang, P.A. Hoyne, T.E. Adams, T.G. Johns, et al. 2004. CR1/CR2 interactions modulate the functions of the cell surface epidermal growth factor receptor. J. Biol. Chem. 279:22387-22398.
10. Moriki, T., H. Maruyama, and I.N. Maruyama. 2001. Activation of preformed EGF receptor dimers by ligand-induced rotation of the transmembrane domain. J. Mol. Biol. 311:1011-1026.
11. Yu, X., K.D. Sharma, T. Takahashi, R. Iwamoto, and E. Mekada. 2002. Ligand-independent dimer formation of epidermal growth factor receptor (EGFR) is a step separable from ligand-induced EGFR signaling. Mol. Biol. Cell 13:2547-2557.
12. Sako, Y., S. Minoghchi, and T. Yanagida. 2000. Single-molecule imaging of EGFR signalling on the surface of living cells. Nat. Cell Biol. 2:168-172.
13. Selvin, P.R. 2000. The renaissance of fluorescence resonance energy transfer. Nat. Struct. Biol. 7:730-734.
14. Pf leger, K.D. and K.A. Eidne. 2006. Illuminating insights into protein-protein interactions using bioluminescence resonance energy transfer (BRET). Nat. Methods 3:165-174.
15. Rossi, F., C.A. Charlton, and H.M. Blau. 1997. Monitoring protein-protein interactions in intact eukaryotic cells by beta-galactosidase complementation. Proc. Natl. Acad. Sci. USA 94:8405-8410.
16. Hall, P.S. and D.A. Cameron. 2009. Current perspective - trastuzumab. Eur. J. Cancer 45:12-18.
17. Schmitz, K.R. and K.M. Ferguson. 2009. Interaction of antibodies with ErbB receptor extracellular regions. Exp. Cell Res. 315:659-670.
18. Burgess, A.W. 2008. EGFR family: structure physiology signalling and therapeutic targets. Growth Factors 26:263-274.
19. Franklin, M.C., K.D. Carey, F.F. Vajdos, D.J. Leahy, A.M. de Vos, and M.X. Sliwkowski. 2004. Insights into ErbB signaling from the structure of the ErbB2-pertuzumab complex. Cancer Cell 5:317-328.
20. Seiden, M.V., H.A. Bur r is, U. Matulonis, J.B. Hall, D.K. Armstrong, J. Speyer, J.D. Weber, and F. Muggia. 2007. A phase II trial of EMD72000 (matuzumab), a humanized anti-EGFR monoclonal antibody, in patients with platinum-resistant ovarian and primary peritoneal malignancies. Gynecol. Oncol. 104:727-731.