A novel method for sensitive and specific detection of DNA methylation biomarkers based on DNA restriction during PCR cycling

BioTechniques

Volumn 47, Issue 3, 2009, Pages 737-743

  Kneip, Christoph ^a   Schmidt, Bernd ^b   Fleischhacker, Michael ^b   Seegebarth, Anke ^a   Lewin, Jörn ^a   Flemming, Nadja ^a   Seemann, Stefanie ^a   Schlegel, Thomas ^a   Witt, Christian ^b   Liebenberg, Volker ^a   Dietrich, Dimo ^a  

^a EPIGENOMICS AG   (Germany)

^b CHARITÉ UNIVERSITÄTSMEDIZIN BERLIN   (Germany)

Author keywords

Cancer; Diagnostics; DNA methylation; Early detection; Quantitative real time PCR; Sensitive detection; Tsp509I

Indexed keywords

BIOLOGICAL MARKER; BISULFITE; HOMEODOMAIN PROTEIN; PROTEIN BARHL2; RESTRICTION ENDONUCLEASE; TSP509I ENDONUCLEASE; UNCLASSIFIED DRUG; BARHL2 PROTEIN, HUMAN; DNA; NERVE PROTEIN; TUMOR MARKER;

ARTICLE; BODY FLUID; CLINICAL ARTICLE; CONTROLLED STUDY; DIGESTION; DNA METHYLATION; DNA RESTRICTION; GENE AMPLIFICATION; HUMAN; LUNG CANCER; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; TRACHEOBRONCHIAL TOILET; CHEMISTRY; EVALUATION STUDY; LUNG LAVAGE; LUNG TUMOR; METABOLISM; POLYMERASE CHAIN REACTION; PROCEDURES;

BRONCHOALVEOLAR LAVAGE FLUID; DNA METHYLATION; DNA RESTRICTION ENZYMES; DNA, NEOPLASM; HOMEODOMAIN PROTEINS; HUMANS; LUNG NEOPLASMS; NERVE TISSUE PROTEINS; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; TUMOR MARKERS, BIOLOGICAL;

EID: 77953848845     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/000113208     Document Type: Article

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