Mechanism of antibody reduction in cell culture production processes

Biotechnology and Bioengineering

Volumn 107, Issue 4, 2010, Pages 622-632

  Kao, Yung Hsiang ^a   Hewitt, Daniel P ^a   Trexler Schmidt, Melody ^a   Laird, Michael W ^a  

^a GENENTECH INC   (United States)

Author keywords

Antibody; Disulfide; Mechanism; Reduction; Thioredoxin

Indexed keywords

ANTIBODY FRAGMENT; DESIGNING METHODS; DISULFIDE; DISULFIDE BONDS; DISULFIDE REDUCTION; EXPRESSION SYSTEM; HARVEST OPERATIONS; HEAVY CHAIN; LIGHT CHAIN; MANUFACTURING PROCESS; MECHANICAL CELLS; PRODUCTION PROCESS; PROTEIN A; PROTEIN A CHROMATOGRAPHY; REDUCING ACTIVITY; SCALE-UP; THERAPEUTIC ANTIBODIES; THIOREDOXINS;

CATALYSTS; CELL CULTURE; ENZYMES; INDUSTRIAL ENGINEERING; LAKES; MANUFACTURE; PRODUCTION ENGINEERING;

ANTIBODIES;

PROTEIN A;

ANIMAL CELL; ANIMAL EXPERIMENT; ANTIBODY DETECTION; ARTICLE; CELL ACTIVITY; CELL CULTURE; CHO CELL; CULTURE MEDIUM; CYTOLYSIS; DISULFIDE BOND; FEMALE; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MECHANICAL STRESS; MOUSE; NONHUMAN; PROTEIN ANALYSIS;

ANIMALS; ANTIBODIES; BIOTECHNOLOGY; CELL CULTURE TECHNIQUES; CHO CELLS; CRICETINAE; CRICETULUS; DISULFIDES; IMMUNOLOGIC FACTORS; OXIDATION-REDUCTION; RECOMBINANT PROTEINS; THIOREDOXINS;

EID: 77953572633     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22848     Document Type: Article

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