Measurement of protein synthesis using heavy water labeling and peptide mass spectrometry: Discrimination between major histocompatibility complex allotypes

Analytical Biochemistry

Volumn 403, Issue 1-2, 2010, Pages 1-12

  De Riva, Alessandra ^a   Deery, Michael J ^a   McDonald, Sarah ^a   Lund, Torben ^b   Busch, Robert ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^b UNIVERSITY COLLEGE LONDON   (United Kingdom)

Author keywords

Allelic polymorphism; Heavy water labeling; Major histocompatibility complex proteins; Mass isotopomer distribution analysis; Peptide liquid chromatography mass spectrometry; Protein synthesis

Indexed keywords

AMINO ACIDS; GENES; HEAVY WATER; ISOMERS; ISOTOPES; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; PEPTIDES; PROTEOMICS;

ANTIGEN PRESENTING CELLS; ISOTOPOMER DISTRIBUTIONS; LIQUID CHROMATOGRAPHY TANDEM MASS SPECTROMETRY (LC MS/MS); LIQUID CHROMATOGRAPHY/MASS SPECTROMETRY; MAJOR HISTOCOMPATIBILITY COMPLEX; NOVEL APPLICATIONS; PROTEIN SYNTHESIS; TRYPTIC PEPTIDES;

BIOSYNTHESIS;

ALANINE; DEUTERIUM OXIDE; GLYCINE; MAJOR HISTOCOMPATIBILITY ANTIGEN CLASS 1; MAJOR HISTOCOMPATIBILITY ANTIGEN CLASS 2; PEPTIDE; PROTEIN;

ANIMAL CELL; ANTIGEN PRESENTING CELL; ARTICLE; CONTROLLED STUDY; HUMAN; HUMAN CELL; IMMUNOPRECIPITATION; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MOUSE; NONHUMAN; PEPTIDE ANALYSIS; PRIORITY JOURNAL; PROTEIN ISOLATION; PROTEIN SYNTHESIS; QUANTITATIVE ANALYSIS; TANDEM MASS SPECTROMETRY; AMINO ACID SEQUENCE; ANIMAL; BAGG ALBINO MOUSE; CELL CULTURE; CELL LINE; MAJOR HISTOCOMPATIBILITY COMPLEX; METABOLISM; MOLECULAR GENETICS; PROCEDURES;

MURINAE;

AMINO ACID SEQUENCE; ANIMALS; ANTIGEN-PRESENTING CELLS; CELL LINE; CELLS, CULTURED; DEUTERIUM OXIDE; HUMANS; MAJOR HISTOCOMPATIBILITY COMPLEX; MASS SPECTROMETRY; MICE; MICE, INBRED BALB C; MOLECULAR SEQUENCE DATA; PEPTIDES; PROTEIN BIOSYNTHESIS; PROTEINS;

EID: 77953358873     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2010.04.018     Document Type: Article

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