Accordance and concordance of PCR and NASBA followed by oligochromatography for the molecular diagnosis of Trypanosoma brucei and Leishmania

Tropical Medicine and International Health

Volumn 15, Issue 7, 2010, Pages 800-805

  Mugasa, Claire M ^a,b   Deborggraeve, Stijn ^c   Schoone, Gerard J ^b   Laurent, Thierry ^d   Leeflang, Mariska M ^b,e   Ekangu, Rosine A ^f   El Safi, Sayda ^g   Saad, Al Farazdag A ^g   Basiye, Frank L ^h   De Doncker, Simone ^c   Lubega, George W ^a   Kager, Piet A ⁱ   Büscher, Philippe ^c   Schallig, Henk D F H ^b  

^a MAKERERE UNIVERSITY   (Uganda)

^b ROYAL TROPICAL INSTITUTE   (Netherlands)

^c INSTITUTE OF TROPICAL MEDICINE   (Belgium)

^d Coris BioConcept ^*  (Belgium)

^e UNIVERSITY OF AMSTERDAM   (Netherlands)

^f Institut National de Recherche Biomedicale   (Congo)

^g UNIVERSITY OF KHARTOUM   (Sudan)

^h KENYA MEDICAL RESEARCH INSTITUTE   (Kenya)

ⁱ ACADEMIC MEDICAL CENTER   (Netherlands)

Author keywords

Diagnostics; Leishmaniasis; Nucleic acid sequence based amplification; Oligochromatography; Polymerase chain reaction; Trypanosomiais

Indexed keywords

ASSAY; BLOOD; CHROMATOGRAPHY; ENDEMISM; ENDOPARASITE; GENETIC ANALYSIS; MOLECULAR ANALYSIS; NUCLEIC ACID; POLYMERASE CHAIN REACTION; PROTOZOAN;

ARTICLE; CHROMATOGRAPHY; CLINICAL TRIAL; HUMAN; LEISHMANIA; MULTICENTER STUDY; NONHUMAN; NUCLEIC ACID SEQUENCE BASED AMPLIFICATION; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; TRYPANOSOMA BRUCEI;

ANIMALS; DNA, PROTOZOAN; HUMANS; LEISHMANIA DONOVANI; LEISHMANIASIS, VISCERAL; POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; SELF-SUSTAINED SEQUENCE REPLICATION; SPECIMEN HANDLING; TRYPANOSOMA BRUCEI GAMBIENSE; TRYPANOSOMIASIS, AFRICAN;

EID: 77953182914     PISSN: 13602276     EISSN: 13653156     Source Type: Journal    
DOI: 10.1111/j.1365-3156.2010.02547.x     Document Type: Article

References (12)

1. Boom R, Sol C, Salimans M, Jansen C, Wertheim van Dillen P Noordaa van der J (1990) Rapid and simple method for purification of nucleic acids. Journal of Clinical Microbiology 28, 495-503.
2. Claes F, Deborggraeve S, Verloo D et al. (2007) Validation of a PCR-Oligochromatography test for detection of Trypanozoon parasites in a multicenter collaborative trial. Journal of Clinical Microbiology 45, 3785-3787.
3. Davison AC Hinkley DV (1997) Bootstrap Methods and Their Application. Cambridge University Press
4. Deborggraeve S, Claes F, Laurent T et al. (2006) Molecular dipstick test for diagnosis of sleeping sickness. Journal of Clinical Microbiology 44, 2884-2889.
5. Deborggraeve S, Laurent T, Espinosa D et al. (2008) A simplified and standardized polymerase chain reaction format for the diagnosis of leishmaniasis. Journal of Infectious Diseases 198, 1565-1572.
6. Langton SD, Chevennement R, Nagelkerke N Lombard B (2002) Analysing collaborative trials for qualitative microbiological methods: accordance and concordance. International Journal of Food Microbiology 79, 175-181.
7. Mugasa CM, Schoone GJ, Ekangu RA, Lubega GW, Kager PA Schallig HDFH (2008) Detection of Trypanosoma brucei parasites in blood using real-time nucleic acid sequence based amplification. Diagnostic Microbiology and Infectious Disease 61, 440-445.
8. Mugasa CM, Laurent T, Schoone GJ, Kager PA, Lubega GW Schallig HDFH (2009) Nucleic acid sequence-based amplification with oligochromatography (NASBA-OC) for the detection of Trypanosoma brucei in clinical samples. Journal of Clinical Microbiology 47, 630-635.
9. Mugasa CM, Laurent T, Schoone GJ et al. (2010) Simplified molecular detection of Leishmania parasites in various clinical samples from patients with leishmaniasis. Parasites and Vectors 3, 13.
10. van der Medier WF, Guerra J, Schoone GJ et al. (2008) Comparison between QT-NASBA, Real-time Reverse-Transcriptase PCR and real-time PCR for quantification of Leishmania parasites. Journal of Clinical Microbiology 46, 73-78.
11. van der Meide WF, Schoone GJ, Faber WR et al. (2005) Quantitative Nucleic Acid Sequence-Based assay as a new molecular tool for detection and quantification of Leishmania parasites in skin biopsy samples. Journal of Clinical Microbiology 43, 5560-5566.
12. van der Voet H van der Raamsdon LWD (2004) Estimation of accordance and concordance in inter-laboratory trials of analytical methods with qualitative results. International Journal of Food Microbiology 95, 231-234.