Quantitative nucleic acid sequence-based assay as a new molecular tool for detection and quantification of Leishmania parasites in skin biopsy samples

Journal of Clinical Microbiology

Volumn 43, Issue 11, 2005, Pages 5560-5566

  Van Der Meide, Wendy F ^a   Schoone, Gerard J ^a   Faber, William R ^b   Zeegelaar, Jim E ^b   De Vries, Henry J C ^b   Özbel, Yusuf ^c   Lai A Fat, Rudy F M ^d   Coelho, Leíla I A R C ^e   Kassi, Masoom ^f   Schallig, Henk D F H ^a  

^a ROYAL TROPICAL INSTITUTE   (Netherlands)

^b ACADEMIC MEDICAL CENTER   (Netherlands)

^c EGE UNIVERSITY   (Turkey)

^d Academic Hospital   (Suriname)

^e FUNDAÇÃO DE MEDICINA TROPICAL DR HEITOR VIEIRA DOURADO   (Brazil)

^f BOLAN MEDICAL COLLEGE   (Pakistan)

Author keywords

[No Author keywords available]

Indexed keywords

RNA 18S;

ADOLESCENT; ADULT; AGED; AMASTIGOTE; ARTICLE; CHILD; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; FEMALE; HUMAN; HUMAN TISSUE; LEISHMANIA; MAJOR CLINICAL STUDY; MALE; NONHUMAN; NUCLEIC ACID SEQUENCE BASED AMPLIFICATION; PARASITE CULTIVATION; PARASITE IDENTIFICATION; PATIENT MONITORING; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROMASTIGOTE; QUANTITATIVE ANALYSIS; QUANTITATIVE NUCLEIC ACID SEQUENCE BASED AMPLIFICATION; SKIN BIOPSY; SKIN LEISHMANIASIS;

ADOLESCENT; ADULT; AGED; ANIMALS; BIOPSY; CHILD; EVALUATION STUDIES; HUMANS; LEISHMANIA; LEISHMANIASIS, CUTANEOUS; MALE; MIDDLE AGED; NUCLEIC ACID AMPLIFICATION TECHNIQUES; RNA, PROTOZOAN; RNA, RIBOSOMAL, 18S; SENSITIVITY AND SPECIFICITY; SKIN;

LEISHMANIA MAJOR;

EID: 27744521261     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.43.11.5560-5566.2005     Document Type: Article

References (34)

1. Alvar, J., C. Canavate, B. Gutierrez-Solar, M. Jimenez, F. Laguna, R. Lopez-Velez, R. Molina, and J. Moreno. 1997. Leishmania and human immunodeficiency virus coinfection: the first 10 years. Clin. Microbiol. Rev. 10:298-319.
2. Berman, J. D. 2005. Recent developments in leishmaniasis: epidemiology, diagnosis, and treatment. Curr. Infect. Dis. Rep. 7:33-38.
3. Boom, R., C. J. A. Sol, M. M. M. Salimans, C. L. Jansen, P. M. E. Wertheimvan Dillen, and J. van der Noordaa. 1990. Rapid and simple method for purification of nucleic acids. J. Clin. Microbiol. 28:495-503.
4. Bossolasco, S., G. Gaiera, D. Olchini, M. Gulletta, L. Martello, A. Bestetti, L. Bossi, L. Germagnoli, A. Lazzarin, C. Uberti-Foppa, and P. Cinque. 2003. Real-time PCR assay for clinical management of human immunodeficiency virus-infected patients with visceral leishmaniasis. J. Clin. Microbiol. 41: 5080-5084.
5. Croft, S. L. 2001. Monitoring drug resistance in leishmaniasis. Trop. Med. Int. Health 6:899-905.
6. de Oliveira, C. I., A. Báfica, F. Oliveira, C. B. F. Favali, T. Correa, L. A. R. Freitas, E. Nascimento, J. M. Costa, and A. Barral. 2003. Clinical utility of polymerase chain reaction-based detection of Leishmania in the diagnosis of American cutaneous leishmaniasis. Clin. Infect. Dis. 37:e149-e153.
7. Faber, W. R., L. Oskam, T. van Gool, N. C. M. Kroon, K. J. Knegt-Junk, H. Hofwegen, A. C. van der Wal, and P. A. Kager. 2003. Value of diagnostic techniques for cutaneous leishmaniasis. J. Am. Acad. Dermatol. 49:70-74.
8. Hepburn, N. C. 2001. Management of cutaneous leishmaniasis. Curr. Opin. Infect. Dis. 14:151-154.
9. Herwaldt, B. L. 1999. Leishmaniasis. Lancet 354:1191-1199.
10. Josephson, K. L., C. P. Gebra, and I. L. Pepper. 1993. Polymerase chain reaction detection of nonviable bacterial pathogens. Appl. Environ. Microbiol. 59:3513-3515.
11. Leon, W., D. L. Fouts, and J. Manning. 1978. Sequence arrangement of the 16S and 26S rRNA genes in pathogenic haemoflagellate Leishmania donovani. Nucleic Acids Res. 5:491-504.
12. Looker, D. L., S. Martinez, J. M. Horton, and J. J. Marr. 1986. Growth of Leishmania donovani amastigotes in the continuous human macrophage cell line U937: studies of drug efficacy and metabolism. J. Infect. Dis. 154:323-327.
13. Mary, C., F. Faraut, L. Lascombe, and H. Dumon. 2004. Quantification of Leishmania infantum DNA by a real-time PCR assay with high sensitivity. J. Clin. Microbiol. 42:5249-5255.
14. Mendonça, M. G., M. E. F. De Brito, E. H. G. Rodrigues, V. Bandeira, M. L. Jardim, and F. G. C. Abath. 2004. Persistence of Leishmania parasites in scars after clinical cure of American cutaneous leishmaniasis: is there a sterile cure? J. Infect. Dis. 189:1018-1023.
15. Meredith, S. E., E. E. Zijlstra, G. J. Schoone, Kroon, N. C. M., G. J. van Eys, K. U. Schaeffer, A. M. el-Hassan, and P. G. Lawyer. 1993. Development and application of the polymerase chain reaction for the detection and identification of Leishmania parasites in clinical material. Arch. Inst. Pasteur Tunis 70:419-431.
16. Omar, S. A., P. F. Mens, G. J. Schoone, A. Yusuf, J. Mwangi, S. Kaniaru, G. A. A. Omer, and H. D. F. H. Schallig. 2005. Plasmodium falciparum: evaluation of a quantitative nucleic acid sequence-based amplification assay to predict the outcome of sulfadoxine-pyrimethamine treatment of uncomplicated malaria. Exp. Parasitol. 110:73-79.
17. Osman, O. F., L. Oskam, E. E. Zijlstra, N. C. M. Kroon, G. J. Schoone, E. A. G. Khalil, M. El-Hassan, and P. A. Kager. 1997. Evaluation of PCR for diagnosis of visceral leishmaniasis. J. Clin. Microbiol. 35:2454-2457.
18. Salman, S., N. G. Rubeiz, and A. G. Kibbi. 1999. Cutaneous leishmaniasis: clinical features and diagnosis. Clin. Dermatol. 17:291-296.
19. Schallig, H. D. F. H., G. J. Schoone, E. J. M. Lommerse, N. C. M. Kroon, P. J. de Vries, and T. van Gool. 2003. Usefulness of quantitative nucleic acid sequence based amplification for diagnosis of malaria in an academic hospital setting. Eur. J. Clin. Microbiol. Infect. Dis. 22:555-557.
20. Schallig, H. D. F. H., G. J. Schoone, N. C. M. Kroon, A. Hailu, F. Chappuis, and H. Veeken. 2001. Development and application of "simple" diagnostic tools for visceral leishmaniasis. Med. Microbiol. Immunol. 190:69-71.
21. Schallig, H. D. F. H., and L. Oskam. 2002. Review: molecular biological applications in the diagnosis and control of leishmaniasis and parasite identification. Trop. Med. Int. Health 7:641-651.
22. Schneider, P., L. Wolters, G. Schoone, H. D. F. H. Schallig, R. Hermsen, P. Sillekens, and R. Sauerwein. 2004. Real-time nucleic acid sequence-based amplification is more convenient than real-time PCR for quantification of Plasmodium falciparum. J. Clin. Microbiol. 43:402-405.
23. Schoone, G. J., L. Oskam, N. C. M. Kroon, H. D. F. H. Schallig, and S. A. Omar. 2000. Detection and quantification of Plasmodium falciparum in blood samples using quantitative nucleic acid sequence-based amplification. J. Clin. Microbiol. 38:4072-4075.
24. Schubach, A., F. Haddad, M. P. Oliveira-Neto, W. Degrave, C. Pirmez, G. Grimaldi, Jr., and O. Fernandes. 1998. Detection of Leishmania DNA by PCR in scars of treated human patients. J. Infect. Dis. 178:911-914.
25. Schulz, A., K. Mellenthin, G. Schönian, B. Fleischer, and C. Drosten. 2003. Detection, differentiation, and quantitation of pathogenic Leishmania organisms by a fluorescence resonance energy transfer-based real-time PCR assay. J. Clin. Microbiol. 41:1529-1535.
26. Smits, H. L., G. C. Gussenhoven, W. Terpstra, R. A. F. Schukkink, B. Van Gemen, and T. Van Gool. 1997. Detection, identification and semi-quantification of malaria parasites by NASBA amplification of small subunit ribosomal RNA sequences. J. Microbiol. Methods 28:65-75.
27. Tavares, C. A. P., A. P. Fernandes, and M. N. Melo. 2003. Molecular diagnosis of leishmaniasis. Expert Rev. Mol. Diagn. 3:89-99.
28. Van der Vliet, G. M. E., P. Schepers, R. A. F. Schukkink, B. van Gemen, and P. R. Klatser. 1994. Assessment of mycobacterial viability by RNA amplification. Antimicrob. Agents Chemother. 38:1959-1965.
29. Van der Vliet, G. M. E., S. Cho, K. Kampirapap, J. van Leeuwen, R. A. F. Schukkink, B. van Gemen, P. K. Das, W. R. Faber, G. P. Walsh, and P. R. Klatser. 1996. Use of NASBA RNA amplification for detection of Mycobacterium leprae in skin biopsies from untreated and treated leprosy patients. Int. J. Lepr. 64:396-403.
30. Van Eys, G. J. J. M., G. J. Schoone, N. C. M. Kroon, and S. B. Ebeling. 1992. Sequence analysis of small subunit ribosomal RNA genes and its use for detection and identification of Leishmania parasites. Mol. Biochem. Parasitol. 51:133-142.
31. Van Gemen, B., R. Van Beuningen, and A. Nabbe. 1994. A one-tube quantitative HIV-1 RNA NASBA nucleic acid amplification assay using electrochemiluminescent (ECL) labelled probes. J. Virol. Methods 49:157-167.
32. Weigle, K. A., L. A. Labrada, C. Lozano, C. Santrich, and D. C. Barker. 2002. PCR-based diagnosis of acute and chronic cutaneous leishmaniasis caused by Leishmania (Viannia). J. Clin. Microbiol. 40:601-606.
33. World Health Organization. 1990. The leishmaniasis. WHO Tech. Rep. Ser. 753:154.
34. Wortmann, G., C. Sweeney, H. S. Houng, N. Aronson, J. Stiteler, J. Jackson, and C. Ockenhouse. 2001. Rapid diagnosis of leishmaniasis by fluorogenic polymerase chain reaction. Am. J. Trop. Med. Hyg. 65:583-587.