Kinesin passing permanent blockages along its protofilament track

Biochemical and Biophysical Research Communications

Volumn 395, Issue 4, 2010, Pages 490-495

  Dreblow, Kerstin ^a   Kalchishkova, Nikolina ^a   Böhm, Konrad J ^a  

^a FRITZ LIPMANN INSTITUTE   (Germany)

Author keywords

Bypassing; Eg5; KIF5A; Motility; Non exchangeable blockages

Indexed keywords

KINESIN;

ANIMAL TISSUE; ARTICLE; BINDING SITE; CHEMICAL LABELING; CONTROLLED STUDY; CYTOSKELETON; ENZYME RELEASE; IMMOBILIZED CELL; INTRACELLULAR TRANSPORT; MICROTUBULE; MOLECULAR SIZE; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN CROSS LINKING; PROTEIN DOMAIN; PROTEIN PROTEIN INTERACTION; PROTEIN TRANSPORT;

ANIMALS; HUMANS; KINESIN; MICROFILAMENTS; MICROTUBULES; PROTEIN TRANSPORT; RECOMBINANT PROTEINS;

EID: 77953125557     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2010.04.035     Document Type: Article

References (30)

1. Hirokawa N., Noda Y., Tanaka Y., and Niwa S. Kinesin superfamily motor proteins and intracellular transport. Nat. Rev. Mol. Cell Biol. 10 (2009) 682-696
2. Hirokawa N., Pfister K.K., Yorifuji H., Wagner M.C., Brady S.T., and Bloom G.S. Submolecular domains of bovine brain kinesin identified by electron microscopy and monoclonal antibody decoration. Cell 56 (1989) 867-878
3. Hancock W.O., and Howard J. Processivity of the motor protein kinesin requires two heads. J. Cell Biol. 140 (1998) 1395-1405
4. Ray S., Meyhöfer E., Milligan R.A., and Howard J. Kinesin follows the microtubule's protofilament axis. J. Cell Biol. 121 (1993) 1083-1093
5. Ray S., Wolf S.G., Howard J., and Downing K.H. Kinesin does not support the motility of zinc-macrotubes. Cell Motil. Cytoskel. 30 (1995) 146-152
6. Hagiwara H., Yorifuji H., Sato-Yoshitake R., and Hirokawa N. Competition between motor molecules (kinesin and cytoplasmic dynein) and fibrous microtubule-associated proteins in binding to microtubules. J. Biol. Chem. 269 (1994) 3581-3589
7. Dixit R., Ross J.L., Goldman Y.E., and Holzbaur E.L. Differential regulation of dynein and kinesin motor proteins by tau. Science 319 (2008) 1086-1089
8. Martin M., Iyadurai S.J., Gassman A., Gindhart J.G., Hays T.S., and Saxton W.M. Cytoplasmic dynein, the dynactin complex, and kinesin are interdependent and essential for fast axonal transport. Mol. Biol. Cell 10 (1999) 3717-3728
9. Seitz A., Kojima H., Oiwa K., Mandelkow E.M., Song Y.H., and Mandelkow E. Single-molecule investigation of the interference between kinesin, tau and MAP2c. EMBO J. 21 (2002) 4896-4905
10. Chrétien D., Metoz F., Verde F., Karsenti E., and Wade R.H. Lattice defects in microtubules: protofilament numbers vary within individual microtubules. J. Cell Biol. 117 (1992) 1031-1040
11. Seitz A., and Surrey T. Processive movement of single kinesins on crowded microtubules visualized using quantum dots. EMBO J. 25 (2006) 267-277
12. Telley I.A., Bieling P., and Surrey T. Obstacles on the microtubule reduce the processivity of kinesin-1 in a minimal in vitro system and in cell extract. Biophys. J. 96 (2009) 3341-3353
13. Korten T., and Diez S. Setting up roadblocks for kinesin-1: mechanism for the selective speed control of cargo carrying microtubules. Lab Chip 8 (2008) 1441-1447
14. Chai Y., Lipowsky R., and Klumpp S. Transport by molecular motors in the presence of static defects. J. Stat. Phys. 135 (2009) 241-260
15. Dreblow K., Kalchishkova N., and Böhm K.J. Kinesin bypassing blockages on microtubule rails. Biophys. Rev. Lett. 4 (2009) 139-151
16. Yildiz A., Tomishige M., Gennerich A., and Vale R.D. Intramolecular strain coordinates kinesin stepping behavior along microtubules. Cell 134 (2008) 1030-1041
17. Shelanski M.L., Gaskin F., and Cantor C.R. Microtubule assembly in the absence of added nucleotides. Proc. Natl. Acad. Sci. USA 70 (1973) 765-768
18. Weingarten M.D., Lockwood A.H., Hwo S.Y., and Kirschner M.W. A protein factor essential for microtubule assembly. Proc. Natl. Acad. Sci. USA 72 (1975) 1858-1862
19. Böhm K.J., Vater W., Fenske H., and Unger E. Effect of microtubule-associated proteins on the protofilament number of microtubules assembled in vitro. Biochim. Biophys. Acta 800 (1984) 119-126
20. Niclas J., Navone F., Hom-Booher N., and Vale R.D. Cloning and localization of a conventional kinesin motor expressed exclusively in neurons. Neuron 12 (1994) 1059-1072
21. Kalchishkova N., and Böhm K.J. The role of kinesin neck linker and neck in velocity regulation. J. Mol. Biol. 382 (2008) 127-135
22. Blangy A., Lane H.A., d'Hérin P., Harper M., Kress M., and Nigg E.A. Phosphorylation by p34cdc2 regulates spindle association of human Eg5, a kinesin-related motor essential for bipolar spindle formation in vivo. Cell 83 (1995) 1159-1169
23. Böhm K.J., Stracke R., Mühlig P., and Unger E. Motorprotein-driven unidirectional transport of micrometer-sized cargoes across isopolar microtubule arrays. Nanotechnology 12 (2001) 238-244
24. Turner D., Chang C., Fang K., Cuomo P., and Murphy D. Kinesin movement on glutaraldehyde-fixed microtubules. Anal. Biochem. 242 (1996) 20-25
25. Boal A.K., Tellez H., Rivera S.B., Miller N.E., Bachand G.D., and Bunker B.C. The stability and functionality of chemically crosslinked microtubules. Small 2 (2006) 793-803
26. Beeg J., Klumpp S., Dimova R., Gracià R.S., Unger E., and Lipowsky R. Transport of beads by several kinesin motors. Biophys. J. 94 (2008) 532-541
27. van den Heuvel M.G.L., and Dekker C. Motor proteins at work for nanotechnology. Science 317 (2007) 333-336
28. Asbury C.L., Fehr A.N., and Block S.M. Kinesin moves by an asymmetric hand-over-hand mechanism. Science 302 (2003) 2130-2134
29. Yildiz A., Tomishige M., Vale R.D., and Selvin P.R. Kinesin walks hand-over-hand. Science 303 (2004) 676-678
30. Bolterauer H., Tuszynski J.A., and Unger E. Directed binding: a novel physical mechanism that describes the directional motion of two-headed kinesin motor proteins. Cell Biochem. Biophys. 42 (1984) 95-119