Indication for a new lipolytic enzyme family: Isolation and characterization of two esterases from a metagenomic library

Journal of Molecular Microbiology and Biotechnology

Volumn 18, Issue 3, 2010, Pages 181-187

  Bayer, Sally ^a   Kunert, Anja ^a   Ballschmiter, Meike ^a   Greiner Stoeffele, Thomas ^a,b  

^a UNIVERSITY OF LEIPZIG   (Germany)

^b c LEcta GmbH   (Germany)

Author keywords

Cluster screening; EC 3.1.1.1; Esterases; Lipolytic enzymes; Metagenome; Rumen

Indexed keywords

BACTERIAL ENZYME; ESTERASE; ESTERASE GK 1; ESTERASE Z 3; GENOMIC DNA; GLUTAMINE DERIVATIVE; GLYCINE DERIVATIVE; GLYCYLHISTIDYLSERYLGLUTAMINYLGLYCINE; HISTIDINE DERIVATIVE; PENTAPEPTIDE; PEPTIDE DERIVATIVE; SERINE; SERYLHISTIDYLSERYLGLUTAMINYLGLYCINE; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL GENE; ENZYME ANALYSIS; ENZYME ISOLATION; ENZYME KINETICS; ESTGK 1 GENE; ESTZ 3 GENE; GENE LIBRARY; METAGENOME; NONHUMAN; NUCLEOTIDE SEQUENCE; PHYLOGENETIC TREE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN EXPRESSION; PROTEIN FAMILY; PROTEIN FUNCTION; PROTEIN MOTIF; RUMINANT STOMACH; SEQUENCE ALIGNMENT; SHEEP;

ACETATES; AMINO ACID MOTIFS; AMINO ACID SEQUENCE; ANIMALS; BUTYRATES; CATALYTIC DOMAIN; CLUSTER ANALYSIS; ESTERASES; GENE LIBRARY; KINETICS; METAGENOME; MOLECULAR SEQUENCE DATA; OCTANOIC ACIDS; PHYLOGENY; RUMEN; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID; SERINE; SHEEP; SUBSTRATE SPECIFICITY;

OVIS ARIES;

EID: 77953125520     PISSN: 14641801     EISSN: None     Source Type: Journal    
DOI: 10.1159/000315459     Document Type: Article

References (23)

1. Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ: Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 1997; 25: 3389-3402.
2. Arpigny JL, Jaeger KE: Bacterial lipolytic enzymes: classification and properties. Biochem J 1999; 343: 177-183.
3. Chu X, He H, Guo C, Sun B: Identification of two novel esterases from a marine metagenomic library derived from South China Sea. Appl Microbiol Biotechnol 2008; 80: 615-625.
4. Elend C, Schmeisser C, Leggewie C, Babiak P, Carballeira JD, Steele HL, et al: Isolation and biochemical characterization of two novel metagenome-derived esterases. Appl Environ Microbiol 2006; 72: 3637-3645.
5. Greiner-Stoeffele T, Struhalla M: Method from the Selection of Biomolecules from Biomolecule Variant Libraries. 2003;DE10350474; WO2005040376.
6. Gupta R, Gupta N, Rathi P: Bacterial lipases: an overview of production, purification and biochemical properties. Appl Microbiol Biotechnol 2004; 64: 763-781.
7. Jeon JH, Kim JT, Kim YJ, Kim HK, Lee HS, Kang SG, et al: Cloning and characterization of a new cold-active lipase from a deep-sea sediment metagenome. Appl Microbiol Biotechnol 2008; 81: 865-874.
8. Kademi A, Ait-Abdelkader N, Fakhreddine L, Baratti J: Purification and characterization of a thermostable esterase from the moderate thermophile Bacillus circulans . Appl Microbiol Biotechnol 2000; 54: 173-179. (Pubitemid 30662725)
9. Kulkarni N, Gadre RV: Production and properties of an alkaline, thermophilic lipase from Pseudomonas fluorescens NS2W. J Ind Microbiol Biotechnol 2002; 28: 344-348.
10. Lee MH, Lee CH, Oh TK, Song JK, Yoon JH: Isolation and characterization of a novel lipase from a metagenomic library of tidal flat sediments: evidence for a new family of bacterial lipases. Appl Environ Microbiol 2006; 72: 7406-7409.
11. Martinelle M, Holmquist M, Hult K: On the interfacial activation of Candida antarctica lipase A and B as compared with Humicola lanuginosa lipase. Biochim Biophys Acta 1995; 1258: 272-276.
12. Rabus R, Ruepp A, Frickey T, et al: The genome of Desulfotalea psychrophila, a sulfate-reducing bacterium from permanently cold Arctic sediments. Environ Microbiol 2004;6:887-902.
13. Rashamuse K, Magomani V, Ronneburg T, Brady D: A novel family VIII carboxylesterase derived from a leachate metagenome library exhibits promiscuous-lactamase activity on nitrocefin. Appl Microbiol Biotechnol 2009; 83: 491-500.
14. Rusnak M, Nieveler J, Schmid RD, Petri R: The putative lipase, AF1763, from Archaeoglobus fulgidusis is a carboxylesterase with a very high pH optimum. Biotechnol Lett 2005; 27: 743-748.
15. Saitou N, Nei M: The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 1987; 4: 406-425.
16. Schrag JD, Li YG, Wu S, Cygler M: Ser-His-Glu triad forms the catalytic site of the lipase from Geotrichum candidum . Nature 1991; 351: 761-764.
17. Seshadri R, Adrian L, Fouts DE, et al: Genome sequence of the PCE-dechlorinating bacterium Dehalococcoides ethenogenes. Science 2005;307:105-108.
18. Sobek H, Gorisch H: Purification and characterization of a heat-stable esterase from the thermoacidophilic archaebacterium, Sulfolobus acidocaldarius. Biochem J 1988; 250: 453-458.
19. Tamura K, Dudley J, Nei M, Kumar S: MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol 2007; 24: 1596-1599.
20. Thompson JD, Higgins DG, Gibson TJ: CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 1994; 22: 4673-4680.
21. Yokogawa T, Suzuki T, Ueda T, Mori M, Ohama T, Kuchino Y, et al: Serine tRNA complementary to the nonuniversal serine codon CUG in Candida cylindracea: evolutionary implications. Proc Natl Acad Sci USA 1992; 89: 7408-7411.
22. Zhang JH, Chung TD, Oldenburg KR: A simple statistical parameter for use in evaluation and validation of high throughput screening assays. J Biomol Screen 1999; 4: 67-73.
23. Zhou J, Bruns MA, Tiedje JM: DNA recovery from soils of diverse composition. Appl Environ Microbiol 1996; 62: 316-322.