Analysis of recombinant human semenogelin as an inhibitor of human sperm motility

Biology of Reproduction

Volumn 82, Issue 3, 2010, Pages 489-496

  Mitra, Anurag ^a   Richardson, Richard T ^a   O'Rand, Michael G ^a  

^a UNIVERSITY OF NORTH CAROLINA SCHOOL OF MEDICINE   (United States)

Author keywords

Contraception; Eppin; Semenogelin; Seminal plasma; Sperm; Sperm motility and transport; Spermatozoa

Indexed keywords

EPPIN; PROSTATE SPECIFIC ANTIGEN; RECOMBINANT PROTEIN; SEMENOGELIN; SEMINAL PLASMA PROTEIN; UNCLASSIFIED DRUG; LEUKOCYTE ELASTASE INHIBITOR; SEMINAL VESICLE SECRETORY PROTEIN; SEMINAL VESICLE SPECIFIC ANTIGEN; SEMINAL VESICLE-SPECIFIC ANTIGEN; SPINLW1 PROTEIN, HUMAN;

AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; HUMAN; PRIORITY JOURNAL; PROTEIN BINDING; SEMINAL PLASMA; SPERMATOZOON MIGRATION; SPERMATOZOON MOTILITY; BINDING SITE; CHEMISTRY; DOSE RESPONSE; DOWN REGULATION; DRUG EFFECT; GENETICS; MALE; METABOLISM; PHYSIOLOGY; PROTEIN TERTIARY STRUCTURE; SITE DIRECTED MUTAGENESIS; SPERMATOZOON; TIME;

BINDING SITES; DOSE-RESPONSE RELATIONSHIP, DRUG; DOWN-REGULATION; HUMANS; MALE; MUTAGENESIS, SITE-DIRECTED; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; PROTEINASE INHIBITORY PROTEINS, SECRETORY; RECOMBINANT PROTEINS; SEMINAL VESICLE SECRETORY PROTEINS; SPERM MOTILITY; SPERMATOZOA; TIME FACTORS;

EID: 77953109845     PISSN: 00063363     EISSN: 15297268     Source Type: Journal    
DOI: 10.1095/biolreprod.109.081331     Document Type: Article

References (25)

1. Robert M, Gagnon C. Purification and characterization of the active precursor of a human sperm motility inhibitor secreted by the seminal vesicles: identity with semenogelin. Biol Reprod 1996; 55:813-821. (Pubitemid 26304757)
2. Robert M, Gagnon C. Semenogelin I: a coagulum forming, multifunctional seminal vesicle protein. Cell Mol Life Sci 1999; 55:944-960. (Pubitemid 29304448)
3. Iwamoto T, Gagnon C. Purification and characterization of a sperm motility inhibitor in human seminal plasma. J Androl 1988; 9:377-383. (Pubitemid 19022030)
4. Iwamoto T, Gagnon C. A human seminal plasma protein blocks the motility of human spermatozoa. J Urol 1988; 140:1045-1048.
5. Luterman M, Iwamoto T, Gagnon C. Origin of the human seminal plasma motility inhibitor within the reproductive tract. Int J Androl 1991; 14:91-98.
6. Robert M, Gagnon C. Sperm motility inhibitor from human seminal plasma: presence of a precursor molecule in seminal vesicle fluid and its molecular processing after ejaculation. Int J Androl 1994; 17:232-240. (Pubitemid 24351549)
7. Robert M, Gagnon C. Sperm motility inhibitor from human seminal plasma: association with semen coagulum. Hum Reprod 1995; 10:2192-2197.
8. Yoshida K, Kawano N, Yoshiike M, Yoshida M, Iwamoto T, Morisawa M. Physiological roles of semenogelin I and zinc in sperm motility and semen coagulation on ejaculation in humans. Mol Hum Reprod 2008; 14: 151-156.
9. Wang Z, Widgren EE, Sivashanmugam P, O'Rand MG, Richardson RT. Association of Eppin with semenogelin on human spermatozoa. Biol Reprod 2005; 72:1064-1070. (Pubitemid 40547968)
10. Robert M, Gibbs BF, Jacobson E, Gagnon C. Characterization of prostate specific antigen proteolytic activity on its major physiological substrate, the sperm motility inhibitor precursor/semenogelin I. Biochemistry 1997; 36:3811-3819. (Pubitemid 27154701)
11. de Lamirande E, Yoshida K, Yoshiike TM, Iwamoto T, Gagnon C. Semenogelin, the main protein of semen coagulum, inhibits human sperm capacitation by interfering with the superoxide anion generated during this process. J Androl 2001; 22:672-679. (Pubitemid 32623087)
12. Richardson RT, Sivashanmugam P, Hall SH, Hamil KG, Moore PA, Ruben SM, French FS, O'Rand MG. Cloning and sequencing of human Eppin: a novel family of protease inhibitors expressed in the epididymis and testis. Gene 2001; 270:93-102.
13. Wang Z, Widgren EE, Richardson RT, O'Rand MG. Characterization of an eppin protein complex from human semen and spermatozoa. Biol Reprod 2007; 77:476-484. (Pubitemid 47328611)
14. O'Rand MG, Widgren EE, Wang Z, Richardson RT. Eppin: an effective target for male contraception. Mol Cell Endocrinol 2006; 250:157-162.
15. Yenugu S, Richardson RT, Sivashanmugam P, Wang Z, O'Rand MG, French FS, Hall SH. Antimicrobial activity of human EPPIN, an androgen regulated sperm bound protein with a whey acidic protein motif. Biol Reprod 2004; 71:1484-1490. (Pubitemid 39403242)
16. O'Rand MG, Widgren EE, Beyler S, Richardson RT. Inhibition of human sperm motility by contraceptive anti-eppin antibodies from infertile male monkeys: effect on cyclic adenosine monophosphate. Biol Reprod 2009; 80:279-285.
17. O'Rand MG, Metz CB. Tests for rabbit sperm surface iron-binding protein and hyaluronidase using the "exchange agglutination" reaction. Biol Reprod 1974; 11:326-334.
18. Bostofte E, Bagger P, Michael A, Stakemann G. Fertility prognosis for infertile men: results of follow-up study of semen analysis in infertile men from two different populations evaluated by the Cox regression model. Fertil Steril 1990; 54:1100-1106.
19. Hirano Y, Shibahara H, Obara H, Suzuki T, Takamizawa S, Yamaguchi C, Tsunoda H, Sato I. Relationships between sperm motility characteristics assessed by the computer-aided sperm analysis (CASA) and fertilization rates in vitro. J Assist Reprod Genet 2001; 18:213-218.
20. Zhao C, Huo R, Wang FQ, Lin M, Zhou ZM, Sha JH. Identification of several proteins involved in regulation of sperm motility by proteomic analysis. Fertil Steril 2007; 87:436-438. (Pubitemid 46176938)
21. Martínez-Heredia J, de Mateo S, Vidal-Taboada JM, Ballescà JL, Oliva R. Identification of proteomic differences in asthenozoospermic sperm samples. Hum Reprod 2008; 23:783-791.
22. Murakami J, Yoshiike M, Satoh M, Furuichi Y, Iwamoto T. Characterization of recombinant precursor proteins of the human seminal plasma sperm motility inhibitor synthesized in insect cells. Int J Mol Med 1998; 2: 693-700.
23. Miyano S, Yoshida K, Yoshiike M, Miyamoto C, Furuichi Y, Iwamoto T. A large deletion of the repeat site in semenogelin I is not involved in male infertility. Int J Mol Med 2003; 11:435-440.
24. Yoshida K, Yamasaki T, Yoshiike M, Takano S, Sato I, Iwamoto T. Quantification of seminal plasma motility inhibitor/semenogelin in human seminal plasma. J Androl 2003; 24:878-884. (Pubitemid 37370544)
25. Yoshida K, Krasznai ZT, Krasznai Z, Yoshiike M, Kawano N, Yoshida M, Morisawa M, Tóth Z, Bazsáné ZK, Márián T, Iwamoto T. Functional implications of membrane modification with semenogelins for inhibition of sperm motility in humans. Cell Motil Cytoskeleton 2009; 66:99-108.